Restriction map for pF1KB9815
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AocI        Aor51HI     
ApoI        AseI        AsnI        Asp700I     Asp718I     
AspI        AviII       AvrII       BalI        BbeI        
BbsI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrBI       BsrFI       BsrGI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcyI          319  GACGCC               G[GA]^CG[CT]C
AflIII        832  ACATGT               A^C[GA][CT]GT
AhdI          367  GACGACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        757  GTGCAC               G^TGCAC
AlwNI         436  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          673  GGGCCC               GGGCC^C
ApaLI         757  GTGCAC               G^TGCAC
AscI          259  GGCGCGCC             GG^CGCGCC
AspEI         367  GACGACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI         388  CACGTG               CAC^GTG
BbvCI          69  CCTCAGC              CCTCAGC(-5/-2)
BfaI           32  CTAG                 C^TAG
BglI          683  GCCCCGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         388  CACGTG               [CT]AC^GT[GA]
BsaHI         319  GACGCC               G[GA]^CG[CT]C
BsiEI         280  CGGCCG               CG[GA][CT]^CG
BsmBI         365  GAGACG               CGTCTC(1/5)
BsmFI         800  GGGAC                GGGAC(10/14)
BspLU11I      832  ACATGT               A^CATGT
BspMI         606  GCAGGT               ACCTGC(4/8)
BsrDI         457  CATTGC               GCAATG(2/0)
BstF5I        201  CATCC                GGATG(2/0)
DdeI           70  CTCAG                C^T[ACGT]AG
EaeI          280  CGGCCG               [CT]^GGCC[GA]
EagI          280  CGGCCG               C^GGCCG
Eam1105I      367  GACGACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          874  GAAGAG               CTCTTC(1/4)
EclXI         280  CGGCCG               C^GGCCG
Eco52I        280  CGGCCG               C^GGCCG
EcoT14I       558  CCTTGG               C^C[AT][AT]GG
FokI          201  CATCC                GGATG(9/13)
HgaI          319  GACGC                GACGC(5/10)
Hin1I         319  GACGCC               G[GA]^CG[CT]C
Ksp632I       874  GAAGAG               CTCTTC(1/4)
MaeI           32  CTAG                 C^TAG
MaeIII        544  GTCAC                ^GT[ACGT]AC
MboII         874  GAAGA                GAAGA(8/7)
PflMI         435  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         388  CACGTG               CAC^GTG
PmlI          388  CACGTG               CAC^GTG
PpuMI         437  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII          72  CAGCTG               CAG^CTG
ScaI          794  AGTACT               AGT^ACT
SfaNI         563  GCATC                GCATC(5/9)
SmaI          138  CCCGGG               CCC^GGG
StyI          558  CCTTGG               C^C[AT][AT]GG
Tsp45I        544  GTCAC                ^GT[GC]AC
TspRI         221  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        435  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         138  CCCGGG               C^CCGGG
XmaI          138  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BamHI          43  GGATCC               G^GATCC
              884  GGATCC              
BanI          465  GGTGCC               G^G[CT][GA]CC
              787  GGTGCC              
BcgI          406  GCAATCAACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              852  GCACATGCCTCG        
BsgI          385  CTGCAC               GTGCAG(16/14)
              850  CTGCAC              
BsmAI         365  GAGAC                GTCTC(1/5)
              818  GAGAC               
BsrI          797  ACTGG                ACTGG(1/-1)
              721  CCAGT               
BssSI         649  CACGAG               CACGAG(-5/-1)
              526  CTCGTG              
BstYI          43  GGATCC               [GA]^GATC[CT]
              884  GGATCC              
DpnII          44  GATC                 ^GATC
              885  GATC                
HphI          449  TCACC                GGTGA(8/7)
              707  TCACC               
MboI           44  GATC                 ^GATC
              885  GATC                
MflI           43  GGATCC               [GA]^GATC[CT]
              884  GGATCC              
NdeII          44  GATC                 ^GATC
              885  GATC                
NlaIII        833  CATG                 CATG^
              855  CATG                
NspI          832  ACATGT               [GA]CATG^[CT]
              854  ACATGC              
SacI           15  GAGCTC               GAGCT^C
              742  GAGCTC              
Sau3AI         44  GATC                 ^GATC
              885  GATC                
TaqI          488  TCGA                 T^CGA
              599  TCGA                
TthHB8I       488  TCGA                 T^CGA
              599  TCGA                
XhoII          43  GGATCC               [GA]^GATC[CT]
              884  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsoBI       Bsp1286I    BssHII      
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DraII       DsaI        EcoO109I    EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        KspI        
MaeII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIV       PstI        
RsaI        SacII       Sau96I      ScrFI       SfcI        
TseI        
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