Restriction map for pF1KB9815
 (Linear) (Six-base) MAPSORT of: KIBB9815.seq  Check: 9896  from: 1  to: 897



KIBB9815.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     597     897 
   Size:        597     300
 
AflIII A'CryG_T

Cuts at:      0     832     897 
   Size:        832      65
 
AhdI GACnn_n'nnGTC

Cuts at:      0     372     897 
   Size:        372     525
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     430     462     897 
   Size:        430      32     435

  Fragments arranged by size: 

                 435     430      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     441     897 
   Size:        441     456
 
ApaI G_GGCC'C

Cuts at:      0     677     897 
   Size:        677     220
 
ApaLI G'TGCA_C

Cuts at:      0     757     897 
   Size:        757     140
 
AscI GG'CGCG_CC

Cuts at:      0     260     897 
   Size:        260     637
 
AvaI C'yCGr_G

Cuts at:      0      12     138     235     630     685     897 
   Size:         12     126      97     395      55     212

  Fragments arranged by size: 

                 395     212     126      97      55      12
 
BamHI G'GATC_C

Cuts at:      0      43     884     897 
   Size:         43     841      13

  Fragments arranged by size: 

                 841      43      13
 
BanI G'GyrC_C

Cuts at:      0     465     787     897 
   Size:        465     322     110

  Fragments arranged by size: 

                 465     322     110
 
BanII G_rGCy'C

Cuts at:      0      19     139     677     746     897 
   Size:         19     120     538      69     151

  Fragments arranged by size: 

                 538     151     120      69      19
 
BbvCI CC'TCA_GC

Cuts at:      0      70     897 
   Size:         70     827
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     395     429     841     875     897 
   Size:        395      34     412      34      22

  Fragments arranged by size: 

                 412     395      34      34      22
 
BglI GCCn_nnn'nGGC

Cuts at:      0     689     897 
   Size:        689     208
 
Bme1580I G_kGCm'C

Cuts at:      0     470     677     694     761     897 
   Size:        470     207      17      67     136

  Fragments arranged by size: 

                 470     207     136      67      17
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     807     897 
   Size:        807      90
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     765     897 
   Size:        765     132
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     463     556     760     897 
   Size:        127     336      93     204     137

  Fragments arranged by size: 

                 336     204     137     127      93
 
Bpu10I CC'TnA_GC

Cuts at:      0      70     897 
   Size:         70     827
 
BsaAI yAC'GTr

Cuts at:      0     390     897 
   Size:        390     507
 
BsaHI Gr'CG_yC

Cuts at:      0     320     897 
   Size:        320     577
 
BseYI C'CCAG_C

Cuts at:      0     226     897 
   Size:        226     671
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     835     897 
   Size:        370     465      62

  Fragments arranged by size: 

                 465     370      62
 
BsiEI CG_ry'CG

Cuts at:      0     283     897 
   Size:        283     614
 
BsiHKAI G_wGCw'C

Cuts at:      0      19     746     761     897 
   Size:         19     727      15     136

  Fragments arranged by size: 

                 727     136      19      15
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     359     897 
   Size:        359     538
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     139     470     677     694     746     761     897 
   Size:         19     120     331     207      17      52      15     136

  Fragments arranged by size: 

                 331     207     136     120      52      19      17      15
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     597     897 
   Size:        597     300
 
BsrDI GCAATG_nn'

Cuts at:      0     456     897 
   Size:        456     441
 
BssHII G'CGCG_C

Cuts at:      0     146     214     260     897 
   Size:        146      68      46     637

  Fragments arranged by size: 

                 637     146      68      46
 
BssSI C'ACGA_G

Cuts at:      0     526     649     897 
   Size:        526     123     248

  Fragments arranged by size: 

                 526     248     123
 
BstYI r'GATC_y

Cuts at:      0      43     884     897 
   Size:         43     841      13

  Fragments arranged by size: 

                 841      43      13
 
BtgI C'CryG_G

Cuts at:      0      20     112     254     897 
   Size:         20      92     142     643

  Fragments arranged by size: 

                 643     142      92      20
 
BtsI GCAGTG_nn'

Cuts at:      0     220     897 
   Size:        220     677
 
EaeI y'GGCC_r

Cuts at:      0     280     897 
   Size:        280     617
 
EagI C'GGCC_G

Cuts at:      0     280     897 
   Size:        280     617
 
EarI CTCTTCn'nnn_

Cuts at:      0     869     897 
   Size:        869      28
 
EcoICRI GAG'CTC

Cuts at:      0      17     744     897 
   Size:         17     727     153

  Fragments arranged by size: 

                 727     153      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     463     556     760     897 
   Size:        127     336      93     204     137

  Fragments arranged by size: 

                 336     204     137     127      93
 
EcoO109I rG'GnC_Cy

Cuts at:      0     125     438     682     897 
   Size:        125     313     244     215

  Fragments arranged by size: 

                 313     244     215     125
 
HaeII r_GCGC'y

Cuts at:      0      38     169     243     272     513     897 
   Size:         38     131      74      29     241     384

  Fragments arranged by size: 

                 384     241     131      74      38      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390     897 
   Size:        358      32     507

  Fragments arranged by size: 

                 507     358      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     897 
   Size:         62     835
 
MspA1I CmG'CkG

Cuts at:      0      22      74     114     256     384     519     569     897 
   Size:         22      52      40     142     128     135      50     328

  Fragments arranged by size: 

                 328     142     135     128      52      50      40      22
            
 
NspI r_CATG'y

Cuts at:      0     836     858     897 
   Size:        836      22      39

  Fragments arranged by size: 

                 836      39      22
 
PasI CC'CwG_GG

Cuts at:      0      39     130     324     897 
   Size:         39      91     194     573

  Fragments arranged by size: 

                 573     194      91      39
 
PciI A'CATG_T

Cuts at:      0     832     897 
   Size:        832      65
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     441     897 
   Size:        441     456
 
PfoI T'CCnGG_A

Cuts at:      0      46     897 
   Size:         46     851
 
PmlI CAC'GTG

Cuts at:      0     390     897 
   Size:        390     507
 
PpuMI rG'GwC_Cy

Cuts at:      0     438     897 
   Size:        438     459
 
PspOMI G'GGCC_C

Cuts at:      0     673     897 
   Size:        673     224
 
PstI C_TGCA'G

Cuts at:      0     292     430     518     897 
   Size:        292     138      88     379

  Fragments arranged by size: 

                 379     292     138      88
 
PvuII CAG'CTG

Cuts at:      0      74     897 
   Size:         74     823
 
SacI G_AGCT'C

Cuts at:      0      19     746     897 
   Size:         19     727     151

  Fragments arranged by size: 

                 727     151      19
 
SacII CC_GC'GG

Cuts at:      0      23     115     257     897 
   Size:         23      92     142     640

  Fragments arranged by size: 

                 640     142      92      23
 
ScaI AGT'ACT

Cuts at:      0     796     897 
   Size:        796     101
 
SfcI C'TryA_G

Cuts at:      0     120     288     426     514     822     897 
   Size:        120     168     138      88     308      75

  Fragments arranged by size: 

                 308     168     138     120      88      75
 
SmaI CCC'GGG

Cuts at:      0     140     897 
   Size:        140     757
 
SrfI GCCC'GGGC

Cuts at:      0     140     897 
   Size:        140     757
 
StyI C'CwwG_G

Cuts at:      0     558     897 
   Size:        558     339
 
TatI w'GTAC_w

Cuts at:      0     794     897 
   Size:        794     103
 
XmaI C'CCGG_G

Cuts at:      0     138     897 
   Size:        138     759
 

 Enzymes that do cut:

     AarI   AflIII     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI
     AvaI    BamHI     BanI    BanII    BbvCI     BcgI     BglI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BsaAI    BsaHI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrDI   BssHII    BssSI
    BstYI     BtgI     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI
 EcoO109I    HaeII    Hin4I     MmeI   MspA1I     NspI     PasI     PciI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI
    SacII     ScaI     SfcI     SmaI     SrfI     StyI     TatI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AleI     AlfI     ApoI     AseI    AsiSI    AvrII     BaeI     BbeI
     BbsI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI
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