Restriction map for pF1KB9805
 (Linear) (Six-base) MAPSORT of: KIBB9805.seq  Check: 3315  from: 1  to: 675



KIBB9805.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AflIII A'CryG_T

Cuts at:      0      29     675 
   Size:         29     646
 
AlwNI CAG_nnn'CTG

Cuts at:      0     471     519     675 
   Size:        471      48     156

  Fragments arranged by size: 

                 471     156      48
 
ApaI G_GGCC'C

Cuts at:      0     133     605     675 
   Size:        133     472      70

  Fragments arranged by size: 

                 472     133      70
 
AvaI C'yCGr_G

Cuts at:      0     132     675 
   Size:        132     543
 
BanI G'GyrC_C

Cuts at:      0     153     485     589     675 
   Size:        153     332     104      86

  Fragments arranged by size: 

                 332     153     104      86
 
BanII G_rGCy'C

Cuts at:      0      11     133     605     675 
   Size:         11     122     472      70

  Fragments arranged by size: 

                 472     122      70      11
 
BbeI G_GCGC'C

Cuts at:      0     157     489     593     675 
   Size:        157     332     104      82

  Fragments arranged by size: 

                 332     157     104      82
 
BglI GCCn_nnn'nGGC

Cuts at:      0      85     137     675 
   Size:         85      52     538

  Fragments arranged by size: 

                 538      85      52
 
BlpI GC'TnA_GC

Cuts at:      0     307     675 
   Size:        307     368
 
Bme1580I G_kGCm'C

Cuts at:      0     133     577     605     675 
   Size:        133     444      28      70

  Fragments arranged by size: 

                 444     133      70      28
 
Bpu10I CC'TnA_GC

Cuts at:      0     176     675 
   Size:        176     499
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      79     302     675 
   Size:         79     223     373

  Fragments arranged by size: 

                 373     223      79
 
BsaHI Gr'CG_yC

Cuts at:      0     154     221     486     550     590     637     675 
   Size:        154      67     265      64      40      47      38

  Fragments arranged by size: 

                 265     154      67      64      47      40      38
 
BseYI C'CCAG_C

Cuts at:      0     171     675 
   Size:        171     504
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     675 
   Size:        143     532
 
BsiEI CG_ry'CG

Cuts at:      0     283     675 
   Size:        283     392
 
BsiHKAI G_wGCw'C

Cuts at:      0      11     675 
   Size:         11     664
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     133     577     605     675 
   Size:         11     122     444      28      70

  Fragments arranged by size: 

                 444     122      70      28      11
 
BsrBI CCG'CTC

Cuts at:      0     206     327     675 
   Size:        206     121     348

  Fragments arranged by size: 

                 348     206     121
 
BssHII G'CGCG_C

Cuts at:      0     319     675 
   Size:        319     356
 
BssSI C'ACGA_G

Cuts at:      0     664     675 
   Size:        664      11
 
BtgI C'CryG_G

Cuts at:      0      48     111     149     675 
   Size:         48      63      38     526

  Fragments arranged by size: 

                 526      63      48      38
 
DraIII CAC_nnn'GTG

Cuts at:      0     572     675 
   Size:        572     103
 
EaeI y'GGCC_r

Cuts at:      0       2     280     521     558     675 
   Size:          2     278     241      37     117

  Fragments arranged by size: 

                 278     241     117      37       2
 
EagI C'GGCC_G

Cuts at:      0     280     675 
   Size:        280     395
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     199     280     675 
   Size:        199      81     395

  Fragments arranged by size: 

                 395     199      81
 
EcoICRI GAG'CTC

Cuts at:      0       9     675 
   Size:          9     666
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     457     505     675 
   Size:        457      48     170

  Fragments arranged by size: 

                 457     170      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     468     516     601     602     675 
   Size:        468      48      85       1      73

  Fragments arranged by size: 

                 468      85      73      48       1
 
FspI TGC'GCA

Cuts at:      0     406     675 
   Size:        406     269
 
HaeII r_GCGC'y

Cuts at:      0     157     489     593     675 
   Size:        157     332     104      82

  Fragments arranged by size: 

                 332     157     104      82
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30     280     312     362     394     675 
   Size:         30     250      32      50      32     281

  Fragments arranged by size: 

                 281     250      50      32      32      30
 
KasI G'GCGC_C

Cuts at:      0     153     485     589     675 
   Size:        153     332     104      86

  Fragments arranged by size: 

                 332     153     104      86
 
MluI A'CGCG_T

Cuts at:      0      29     675 
   Size:         29     646
 
MspA1I CmG'CkG

Cuts at:      0     113     151     194     234     294     675 
   Size:        113      38      43      40      60     381

  Fragments arranged by size: 

                 381     113      60      43      40      38
 
NarI GG'CG_CC

Cuts at:      0     154     486     590     675 
   Size:        154     332     104      85

  Fragments arranged by size: 

                 332     154     104      85
 
NotI GC'GGCC_GC

Cuts at:      0     280     675 
   Size:        280     395
 
NspI r_CATG'y

Cuts at:      0     339     675 
   Size:        339     336
 
PasI CC'CwG_GG

Cuts at:      0     597     675 
   Size:        597      78
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     519     675 
   Size:        519     156
 
PfoI T'CCnGG_A

Cuts at:      0     631     675 
   Size:        631      44
 
PspOMI G'GGCC_C

Cuts at:      0     129     601     675 
   Size:        129     472      74

  Fragments arranged by size: 

                 472     129      74
 
SacI G_AGCT'C

Cuts at:      0      11     675 
   Size:         11     664
 
SacII CC_GC'GG

Cuts at:      0     114     152     675 
   Size:        114      38     523

  Fragments arranged by size: 

                 523     114      38
 
SfoI GGC'GCC

Cuts at:      0     155     487     591     675 
   Size:        155     332     104      84

  Fragments arranged by size: 

                 332     155     104      84
 
SmaI CCC'GGG

Cuts at:      0     134     675 
   Size:        134     541
 
SmlI C'TyrA_G

Cuts at:      0      10     675 
   Size:         10     665
 
SphI G_CATG'C

Cuts at:      0     339     675 
   Size:        339     336
 
SrfI GCCC'GGGC

Cuts at:      0     134     675 
   Size:        134     541
 
XmaI C'CCGG_G

Cuts at:      0     132     675 
   Size:        132     543
 

 Enzymes that do cut:

   AflIII    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BglI
     BlpI Bme1580I   Bpu10I     BsaI    BsaHI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BsrBI   BssHII    BssSI     BtgI   DraIII     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I     FspI    HaeII    Hin4I
     KasI     MluI   MspA1I     NarI     NotI     NspI     PasI    PflMI
     PfoI   PspOMI     SacI    SacII     SfoI     SmaI     SmlI     SphI
     SrfI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EarI  Eco57MI    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MmeI     MscI     MslI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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