Restriction map for pF1KB9797
 (Circular) (Six-base) MAPSORT of: pF1KB9797.seq  Check: 8187  from: 1  to: 3413



pF1KB9797  3413 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    451     451 
   Size:       3413
 
Acc65I G'GTAC_C

Cuts at:    429     429 
   Size:       3413
 
AclI AA'CG_TT

Cuts at:   3253    3253 
   Size:       3413
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    217    1238    1670    2417    2685     217 
   Size:       1021     432     747     268     945

  Fragments arranged by size: 

                1021     945     747     432     268
 
AflIII A'CryG_T

Cuts at:    765    1869    2846     765 
   Size:       1104     977    1332

  Fragments arranged by size: 

                1332    1104     977
 
AgeI A'CCGG_T

Cuts at:   1801    1801 
   Size:       3413
 
AlwNI CAG_nnn'CTG

Cuts at:    228     718    2285     228 
   Size:        490    1567    1356

  Fragments arranged by size: 

                1567    1356     490
 
ApaLI G'TGCA_C

Cuts at:   2183    2183 
   Size:       3413
 
ApoI r'AATT_y

Cuts at:    187     213     247     352     417    2766    3022     187 
   Size:         26      34     105      65    2349     256     578

  Fragments arranged by size: 

                2349     578     256     105      65      34      26
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3413
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3413
 
AvaI C'yCGr_G

Cuts at:    386     433     386 
   Size:         47    3366
 
BamHI G'GATC_C

Cuts at:    438     438 
   Size:       3413
 
BanI G'GyrC_C

Cuts at:    429     843    1091    1126     429 
   Size:        414     248      35    2716

  Fragments arranged by size: 

                2716     414     248      35
 
BanII G_rGCy'C

Cuts at:    363     427    1457     363 
   Size:         64    1030    2319

  Fragments arranged by size: 

                2319    1030      64
 
BbeI G_GCGC'C

Cuts at:    847    1095     847 
   Size:        248    3165
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3379
 
BciVI GTATCCnnnnn_n'

Cuts at:   1311    2072    3413    1311 
   Size:        761    1341    1311

  Fragments arranged by size: 

                1341    1311     761
 
BclI T'GATC_A

Cuts at:    933     933 
   Size:       3413
 
BglII A'GATC_T

Cuts at:    928    2965     928 
   Size:       2037    1376
 
BlpI GC'TnA_GC

Cuts at:    525     525 
   Size:       3413
 
Bme1580I G_kGCm'C

Cuts at:   1038    1131    2187    1038 
   Size:         93    1056    2264

  Fragments arranged by size: 

                2264    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    625     706    1041    3185     625 
   Size:         81     335    2144     853

  Fragments arranged by size: 

                2144     853     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1780    1780 
   Size:       3413
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    589    1468    1960    2258     589 
   Size:        879     492     298    1744

  Fragments arranged by size: 

                1744     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3117    3117 
   Size:       3413
 
BsaAI yAC'GTr

Cuts at:    766     766 
   Size:       3413
 
BsaBI GATnn'nnATC

Cuts at:      6     927    2964       6 
   Size:        921    2037     455

  Fragments arranged by size: 

                2037     921     455
 
BsaHI Gr'CG_yC

Cuts at:    844    1092    1794    3290     844 
   Size:        248     702    1496     967

  Fragments arranged by size: 

                1496     967     702     248
 
BsaWI w'CCGG_w

Cuts at:    602    1123    1801    2075    2222    2633     602 
   Size:        521     678     274     147     411    1382

  Fragments arranged by size: 

                1382     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    838    1596    2173    2669     838 
   Size:        758     577     496    1582

  Fragments arranged by size: 

                1582     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    168     168 
   Size:       3413
 
BsiEI CG_ry'CG

Cuts at:    104     955    1001    2209     104 
   Size:        851      46    1208    1308

  Fragments arranged by size: 

                1308    1208     851      46
 
BsiHKAI G_wGCw'C

Cuts at:    427    1205    1395    2187     427 
   Size:        778     190     792    1653

  Fragments arranged by size: 

                1653     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    366     381     366 
   Size:         15    3398
 
Bsp1286I G_dGCh'C

Cuts at:    363     427    1038    1131    1205    1395    1457    2187     363 
   Size:         64     611      93      74     190      62     730    1589

  Fragments arranged by size: 

                1589     730     611     190      93      74      64      62
            
 
BspEI T'CCGG_A

Cuts at:   2633    2633 
   Size:       3413
 
BspHI T'CATG_A

Cuts at:   2589    2589 
   Size:       3413
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    463     677     979    1360     463 
   Size:        214     302     381    2516

  Fragments arranged by size: 

                2516     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1705    1759    3243    3408      50 
   Size:       1655      54    1484     165      55

  Fragments arranged by size: 

                1655    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1325    1325 
   Size:       3413
 
BsrFI r'CCGG_y

Cuts at:   1411    1592    1801    1411 
   Size:        181     209    3023

  Fragments arranged by size: 

                3023     209     181
 
BssSI C'ACGA_G

Cuts at:   1684    2042    1684 
   Size:        358    3055
 
BstAPI GCAn_nnn'nTGC

Cuts at:    790     790 
   Size:       3413
 
BstBI TT'CG_AA

Cuts at:   1774    1774 
   Size:       3413
 
BstYI r'GATC_y

Cuts at:      1     438     905     928    1263    1509    2521    2607    2619 
   Size:        437     467      23     335     246    1012      86      12

Cuts at:   2619    2965       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     449     437     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1363    1544    1707    1363 
   Size:        181     163    3069

  Fragments arranged by size: 

                3069     181     163
 
BtsI GCAGTG_nn'

Cuts at:    656     656 
   Size:       3413
 
DraI TTT'AAA

Cuts at:    252     279     412     252 
   Size:         27     133    3253

  Fragments arranged by size: 

                3253     133      27
 
DrdI GACnn_nn'nnGTC

Cuts at:   1119    1977    1119 
   Size:        858    2555
 
EaeI y'GGCC_r

Cuts at:    998    1172    1563    1590    2935     998 
   Size:        174     391      27    1345    1476

  Fragments arranged by size: 

                1476    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    998     998 
   Size:       3413
 
EarI CTCTTCn'nnn_

Cuts at:    323    1436    1646     323 
   Size:       1113     210    2090

  Fragments arranged by size: 

                2090    1113     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1621    1931    2077    3223    1621 
   Size:        310     146    1146    1811

  Fragments arranged by size: 

                1811    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    425     425 
   Size:       3413
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    217    1238    1670    2417    2685     217 
   Size:       1021     432     747     268     945

  Fragments arranged by size: 

                1021     945     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    552     552 
   Size:       3413
 
EcoRI G'AATT_C

Cuts at:    417     417 
   Size:       3413
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1432    1432 
   Size:       3413
 
FspI TGC'GCA

Cuts at:   1194    1194 
   Size:       3413
 
HaeII r_GCGC'y

Cuts at:    847    1095    2117    3096     847 
   Size:        248    1022     979    1164

  Fragments arranged by size: 

                1164    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    324     356    1255    1285    1287    1317     324 
   Size:         32     899      30       2      30    2420

  Fragments arranged by size: 

                2420     899      32      30      30       2
 
HincII GTy'rAC

Cuts at:    452     452 
   Size:       3413
 
KasI G'GCGC_C

Cuts at:    843    1091     843 
   Size:        248    3165
 
KpnI G_GTAC'C

Cuts at:    433     433 
   Size:       3413
 
MluI A'CGCG_T

Cuts at:   2846    2846 
   Size:       3413
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2084    2268    2084 
   Size:        184    3229
 
MscI TGG'CCA

Cuts at:   1174    1174 
   Size:       3413
 
MslI CAynn'nnrTG

Cuts at:   1402    1529    1402 
   Size:        127    3286
 
MspA1I CmG'CkG

Cuts at:    524     838    1198    2211    2456     524 
   Size:        314     360    1013     245    1481

  Fragments arranged by size: 

                1481    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1594    1594 
   Size:       3413
 
NarI GG'CG_CC

Cuts at:    844    1092     844 
   Size:        248    3165
 
NgoMIV G'CCGG_C

Cuts at:   1592    1592 
   Size:       3413
 
NspI r_CATG'y

Cuts at:    466     966    1873     466 
   Size:        500     907    2006

  Fragments arranged by size: 

                2006     907     500
 
PciI A'CATG_T

Cuts at:   1869    1869 
   Size:       3413
 
PmeI GTTT'AAAC

Cuts at:    412     412 
   Size:       3413
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2552    2584    2552 
   Size:         32    3381
 
PstI C_TGCA'G

Cuts at:    460    1145     460 
   Size:        685    2728
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3413
 
PvuII CAG'CTG

Cuts at:    838    1198     838 
   Size:        360    3053
 
RsrII CG'GwC_CG

Cuts at:   1608    1608 
   Size:       3413
 
SacI G_AGCT'C

Cuts at:    427     427 
   Size:       3413
 
SalI G'TCGA_C

Cuts at:    450     450 
   Size:       3413
 
SapI GCTCTTCn'nnn_

Cuts at:   1436    1646    1436 
   Size:        210    3203
 
SbfI CC_TGCA'GG

Cuts at:    460     460 
   Size:       3413
 
ScaI AGT'ACT

Cuts at:   2930    2930 
   Size:       3413
 
SfcI C'TryA_G

Cuts at:     33     267     456    1141    2134    2325      33 
   Size:        234     189     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     234     191     189
 
SfoI GGC'GCC

Cuts at:    845    1093     845 
   Size:        248    3165
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3413
 
SmaI CCC'GGG

Cuts at:    435     435 
   Size:       3413
 
SmlI C'TyrA_G

Cuts at:    568    1483    1975    2237     568 
   Size:        915     492     262    1744

  Fragments arranged by size: 

                1744     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3413
 
SphI G_CATG'C

Cuts at:    466     966     466 
   Size:        500    2913
 
StyI C'CwwG_G

Cuts at:    547     901     547 
   Size:        354    3059
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    996    1129    1588    1797     996 
   Size:        133     459     209    2612

  Fragments arranged by size: 

                2612     459     209     133
 
TatI w'GTAC_w

Cuts at:   2928    2928 
   Size:       3413
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2930    2962    2930 
   Size:         32    3381
 
Tth111I GACn'n_nGTC

Cuts at:   1210    1210 
   Size:       3413
 
XbaI T'CTAG_A

Cuts at:    444     444 
   Size:       3413
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    908     908 
   Size:       3413
 
XmaI C'CCGG_G

Cuts at:    433     433 
   Size:       3413
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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