Restriction map for pF1KB9791
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        DpnII       DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MboI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        NarI        
NcoI        NdeI        NdeII       NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        SacI        SacII       SalI        Sau3AI      
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccII         346  CGCG                 CG^CG
AfaI          402  GTAC                 GT^AC
AlwNI         300  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          163  GGGCCC               GGGCC^C
BanII         163  GGGCCC               G[GA]GC[CT]^C
BcgI          366  CGAGGTGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          146  CTAG                 C^TAG
BpmI          184  CTGGAG               CTGGAG(16/14)
BsrDI          89  CATTGC               GCAATG(2/0)
BsrFI         233  GCCGGC               [GA]^CCGG[CT]
BstBI         578  TTCGAA               TT^CGAA
BstF5I         30  CATCC                GGATG(2/0)
BstNI         552  CCAGG                CC^[AT]GG
BstUI         346  CGCG                 CG^CG
CfoI          345  GCGC                 GCG^C
Cfr10I        233  GCCGGC               [GA]^CCGG[CT]
CpoI          322  CGGACCG              CG^G[AT]CCG
EarI          175  CTCTTC               CTCTTC(1/4)
EcoNI         180  CCTGCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        552  CCAGG                ^CC[AT]GG
FokI           30  CATCC                GGATG(9/13)
HgaI          596  GCGTC                GACGC(5/10)
HhaI          345  GCGC                 GCG^C
HinP1I        345  GCGC                 G^CGC
Ksp632I       175  CTCTTC               CTCTTC(1/4)
MaeI          146  CTAG                 C^TAG
MaeIII        310  GTGAC                ^GT[ACGT]AC
MboII         176  TCTTC                GAAGA(8/7)
MfeI           62  CAATTG               C^AATTG
MunI           62  CAATTG               C^AATTG
MvaI          552  CCAGG                CC^[AT]GG
MvnI          346  CGCG                 CG^CG
NaeI          233  GCCGGC               GCC^GGC
NgoMIV        233  GCCGGC               G^CCGGC
NlaIII         17  CATG                 CATG^
NspV          578  TTCGAA               TT^CGAA
PpuMI         116  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          374  CTGCAG               CTGCA^G
PvuII         377  CAGCTG               CAG^CTG
RsaI          402  GTAC                 GT^AC
RsrII         322  CGGACCG              CG^G[AT]CCG
SapI          174  GCTCTTC              GCTCTTC(1/4)
ScaI          401  AGTACT               AGT^ACT
SfcI          374  CTGCAG               C^T[GA][CT]AG
SfuI          578  TTCGAA               TT^CGAA
SmaI          450  CCCGGG               CCC^GGG
TaqI          579  TCGA                 T^CGA
TfiI          576  GATTC                G^A[AT]TC
Tsp45I        310  GTGAC                ^GT[GC]AC
TthHB8I       579  TCGA                 T^CGA
XmaCI         450  CCCGGG               C^CCGGG
XmaI          450  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          278  AGCT                 AG^CT
              378  AGCT                
AvaI          131  CCCGAG               C^[CT]CG[GA]G
              450  CCCGGG              
AvaII         117  GGACC                G^G[AT]CC
              323  GGACC               
BbvCI         609  CCTCAGC              CCTCAGC(-5/-2)
              436  GCTGAGG             
BglI          557  GCCGTGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              614  GCCGGACTGGC         
BsgI          254  CTGCAC               GTGCAG(16/14)
              600  CTGCAC              
BsoBI         131  CCCGAG               C^[CT]CG[GA]G
              450  CCCGGG              
BspMI         211  GCAGGT               ACCTGC(4/8)
              457  GCAGGT              
EaeI          221  CGGCCA               [CT]^GGCC[GA]
              621  TGGCCG              
FauI          416  CCCGC                CCCGC(4/6)
              531  GCGGG               
SfaNI         292  GATGC                GCATC(5/9)
              547  GATGC               
Tsp509I        63  AATT                 ^AATT
              582  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        BanI        BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       Cac8I       Cfr13I      
DdeI        DraII       EcoO109I    Fnu4HI      HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MnlI        MspA1I      MspI        MwoI        NciI        
NlaIV       PleI        Sau96I      ScrFI       TseI        
TspRI       
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