Restriction map for pF1KB9791
 (Linear) (Six-base) MAPSORT of: KIBB9791.seq  Check: 9291  from: 1  to: 630



KIBB9791.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     202     448     630 
   Size:        202     246     182

  Fragments arranged by size: 

                 246     202     182
 
AlwNI CAG_nnn'CTG

Cuts at:      0     305     630 
   Size:        305     325
 
ApaI G_GGCC'C

Cuts at:      0     167     630 
   Size:        167     463
 
AvaI C'yCGr_G

Cuts at:      0     131     450     630 
   Size:        131     319     180

  Fragments arranged by size: 

                 319     180     131
 
BanI G'GyrC_C

Cuts at:      0     151     214     369     630 
   Size:        151      63     155     261

  Fragments arranged by size: 

                 261     155     151      63
 
BanII G_rGCy'C

Cuts at:      0     167     630 
   Size:        167     463
 
BbvCI CC'TCA_GC

Cuts at:      0     437     610     630 
   Size:        437     173      20

  Fragments arranged by size: 

                 437     173      20
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     355     389     630 
   Size:        355      34     241

  Fragments arranged by size: 

                 355     241      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     563     620     630 
   Size:        563      57      10

  Fragments arranged by size: 

                 563      57      10
 
Bme1580I G_kGCm'C

Cuts at:      0     156     167     219     630 
   Size:        156      11      52     411

  Fragments arranged by size: 

                 411     156      52      11
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      47     504     630 
   Size:         47     457     126

  Fragments arranged by size: 

                 457     126      47
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     630 
   Size:        205     425
 
Bpu10I CC'TnA_GC

Cuts at:      0     437     610     630 
   Size:        437     173      20

  Fragments arranged by size: 

                 437     173      20
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     109     139     630 
   Size:        109      30     491

  Fragments arranged by size: 

                 491     109      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25      37     598     630 
   Size:         25      12     561      32

  Fragments arranged by size: 

                 561      32      25      12
 
BseYI C'CCAG_C

Cuts at:      0     160     190     305     630 
   Size:        160      30     115     325

  Fragments arranged by size: 

                 325     160     115      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     585     630 
   Size:        239     346      45

  Fragments arranged by size: 

                 346     239      45
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     167     219     630 
   Size:        156      11      52     411

  Fragments arranged by size: 

                 411     156      52      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     202     448     630 
   Size:        202     246     182

  Fragments arranged by size: 

                 246     202     182
 
BsrDI GCAATG_nn'

Cuts at:      0      88     630 
   Size:         88     542
 
BsrFI r'CCGG_y

Cuts at:      0     233     630 
   Size:        233     397
 
BstBI TT'CG_AA

Cuts at:      0     579     630 
   Size:        579      51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     305     585     630 
   Size:        305     280      45

  Fragments arranged by size: 

                 305     280      45
 
BtsI GCAGTG_nn'

Cuts at:      0     587     630 
   Size:        587      43
 
EaeI y'GGCC_r

Cuts at:      0     221     621     630 
   Size:        221     400       9

  Fragments arranged by size: 

                 400     221       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     181     630 
   Size:        181     449
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     385     630 
   Size:        385     245
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     630 
   Size:        205     425
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     184     630 
   Size:        184     446
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117     164     194     630 
   Size:        117      47      30     436

  Fragments arranged by size: 

                 436     117      47      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     109     141     630 
   Size:        109      32     489

  Fragments arranged by size: 

                 489     109      32
 
MfeI C'AATT_G

Cuts at:      0      62     630 
   Size:         62     568
 
MspA1I CmG'CkG

Cuts at:      0     328     379     419     544     630 
   Size:        328      51      40     125      86

  Fragments arranged by size: 

                 328     125      86      51      40
 
NaeI GCC'GGC

Cuts at:      0     235     630 
   Size:        235     395
 
NgoMIV G'CCGG_C

Cuts at:      0     233     630 
   Size:        233     397
 
PasI CC'CwG_GG

Cuts at:      0     552     630 
   Size:        552      78
 
PpuMI rG'GwC_Cy

Cuts at:      0     117     630 
   Size:        117     513
 
PspOMI G'GGCC_C

Cuts at:      0     163     630 
   Size:        163     467
 
PstI C_TGCA'G

Cuts at:      0     378     630 
   Size:        378     252
 
PvuII CAG'CTG

Cuts at:      0     379     630 
   Size:        379     251
 
RsrII CG'GwC_CG

Cuts at:      0     323     630 
   Size:        323     307
 
SapI GCTCTTCn'nnn_

Cuts at:      0     181     630 
   Size:        181     449
 
ScaI AGT'ACT

Cuts at:      0     403     630 
   Size:        403     227
 
SfcI C'TryA_G

Cuts at:      0     374     630 
   Size:        374     256
 
SmaI CCC'GGG

Cuts at:      0     452     630 
   Size:        452     178
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     297     630 
   Size:        297     333
 
TatI w'GTAC_w

Cuts at:      0     401     630 
   Size:        401     229
 
XmaI C'CCGG_G

Cuts at:      0     450     630 
   Size:        450     180
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI     AvaI     BanI    BanII    BbvCI     BcgI
     BglI Bme1580I     BmrI     BpmI   Bpu10I    BsaXI    BseRI    BseYI
     BsgI Bsp1286I    BspMI    BsrDI    BsrFI    BstBI    BtgZI     BtsI
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    Hin4I     MfeI
   MspA1I     NaeI   NgoMIV     PasI    PpuMI   PspOMI     PstI    PvuII
    RsrII     SapI     ScaI     SfcI     SmaI    TaqII     TatI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI     SacI    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI
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