Restriction map for pF1KB9784
 (Linear) (Six-base) MAPSORT of: KIBB9784.seq  Check: 7383  from: 1  to: 567



KIBB9784.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     462     530     567 
   Size:        462      68      37

  Fragments arranged by size: 

                 462      68      37
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     567 
   Size:        291     276
 
ApaI G_GGCC'C

Cuts at:      0      36     197     439     567 
   Size:         36     161     242     128

  Fragments arranged by size: 

                 242     161     128      36
 
AvaI C'yCGr_G

Cuts at:      0      61      96     137     567 
   Size:         61      35      41     430

  Fragments arranged by size: 

                 430      61      41      35
 
BanI G'GyrC_C

Cuts at:      0      26     562     567 
   Size:         26     536       5

  Fragments arranged by size: 

                 536      26       5
 
BanII G_rGCy'C

Cuts at:      0      36     156     197     439     567 
   Size:         36     120      41     242     128

  Fragments arranged by size: 

                 242     128     120      41      36
 
BbeI G_GCGC'C

Cuts at:      0      30     567 
   Size:         30     537
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     567 
   Size:         10     557
 
BglI GCCn_nnn'nGGC

Cuts at:      0      24     286     419     502     567 
   Size:         24     262     133      83      65

  Fragments arranged by size: 

                 262     133      83      65      24
 
Bme1580I G_kGCm'C

Cuts at:      0      36     197     439     565     567 
   Size:         36     161     242     126       2

  Fragments arranged by size: 

                 242     161     126      36       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     445     567 
   Size:        253     192     122

  Fragments arranged by size: 

                 253     192     122
 
BsaAI yAC'GTr

Cuts at:      0     312     567 
   Size:        312     255
 
BsaHI Gr'CG_yC

Cuts at:      0      27      73     567 
   Size:         27      46     494

  Fragments arranged by size: 

                 494      46      27
 
BseYI C'CCAG_C

Cuts at:      0     478     506     567 
   Size:        478      28      61

  Fragments arranged by size: 

                 478      61      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     567 
   Size:         61     506
 
BsiEI CG_ry'CG

Cuts at:      0     133     567 
   Size:        133     434
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     337     567 
   Size:        337     230
 
Bsp1286I G_dGCh'C

Cuts at:      0      36     156     197     439     565     567 
   Size:         36     120      41     242     126       2

  Fragments arranged by size: 

                 242     126     120      41      36       2
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     462     530     567 
   Size:        462      68      37

  Fragments arranged by size: 

                 462      68      37
 
BsrBI CCG'CTC

Cuts at:      0     252     567 
   Size:        252     315
 
BsrFI r'CCGG_y

Cuts at:      0      14     108     128     132     567 
   Size:         14      94      20       4     435

  Fragments arranged by size: 

                 435      94      20      14       4
 
BssHII G'CGCG_C

Cuts at:      0     315     567 
   Size:        315     252
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     261     567 
   Size:        261     306
 
BstYI r'GATC_y

Cuts at:      0     542     567 
   Size:        542      25
 
BtgI C'CryG_G

Cuts at:      0      22     497     567 
   Size:         22     475      70

  Fragments arranged by size: 

                 475      70      22
 
EaeI y'GGCC_r

Cuts at:      0     110     130     178     201     356     383     567 
   Size:        110      20      48      23     155      27     184

  Fragments arranged by size: 

                 184     155     110      48      27      23      20
 
EagI C'GGCC_G

Cuts at:      0     130     567 
   Size:        130     437
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     445     567 
   Size:        253     192     122

  Fragments arranged by size: 

                 253     192     122
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33      83     184     288     436     567 
   Size:         33      50     101     104     148     131

  Fragments arranged by size: 

                 148     131     104     101      50      33
 
FseI GG_CCGG'CC

Cuts at:      0     136     567 
   Size:        136     431
 
HaeII r_GCGC'y

Cuts at:      0      30     567 
   Size:         30     537
 
KasI G'GCGC_C

Cuts at:      0      26     567 
   Size:         26     541
 
MscI TGG'CCA

Cuts at:      0     358     567 
   Size:        358     209
 
MspA1I CmG'CkG

Cuts at:      0     225     258     567 
   Size:        225      33     309

  Fragments arranged by size: 

                 309     225      33
 
NaeI GCC'GGC

Cuts at:      0     110     130     134     567 
   Size:        110      20       4     433

  Fragments arranged by size: 

                 433     110      20       4
 
NarI GG'CG_CC

Cuts at:      0      27     567 
   Size:         27     540
 
NgoMIV G'CCGG_C

Cuts at:      0     108     128     132     567 
   Size:        108      20       4     435

  Fragments arranged by size: 

                 435     108      20       4
 
PasI CC'CwG_GG

Cuts at:      0     513     567 
   Size:        513      54
 
PmlI CAC'GTG

Cuts at:      0     312     567 
   Size:        312     255
 
PpuMI rG'GwC_Cy

Cuts at:      0      83     184     567 
   Size:         83     101     383

  Fragments arranged by size: 

                 383     101      83
 
PspOMI G'GGCC_C

Cuts at:      0      32     193     435     567 
   Size:         32     161     242     132

  Fragments arranged by size: 

                 242     161     132      32
 
PstI C_TGCA'G

Cuts at:      0     527     567 
   Size:        527      40
 
SanDI GG'GwC_CC

Cuts at:      0      83     567 
   Size:         83     484
 
SfcI C'TryA_G

Cuts at:      0     523     567 
   Size:        523      44
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     502     567 
   Size:        502      65
 
SfoI GGC'GCC

Cuts at:      0      28     567 
   Size:         28     539
 
SmaI CCC'GGG

Cuts at:      0      63      98     139     567 
   Size:         63      35      41     428

  Fragments arranged by size: 

                 428      63      41      35
 
SrfI GCCC'GGGC

Cuts at:      0     139     567 
   Size:        139     428
 
StuI AGG'CCT

Cuts at:      0     422     567 
   Size:        422     145
 
StyI C'CwwG_G

Cuts at:      0     113     567 
   Size:        113     454
 
Tth111I GACn'n_nGTC

Cuts at:      0     399     567 
   Size:        399     168
 
XmaI C'CCGG_G

Cuts at:      0      61      96     137     567 
   Size:         61      35      41     430

  Fragments arranged by size: 

                 430      61      41      35
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BbsI
     BglI Bme1580I     BpmI    BsaAI    BsaHI    BseYI     BsgI    BsiEI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstYI
     BtgI     EaeI     EagI  Eco57MI EcoO109I     FseI    HaeII     KasI
     MscI   MspA1I     NaeI     NarI   NgoMIV     PasI     PmlI    PpuMI
   PspOMI     PstI    SanDI     SfcI     SfiI     SfoI     SmaI     SrfI
     StuI     StyI  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MslI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI     SwaI
    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmnI     ZraI
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