Restriction map for pF1KB9747
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AgeI        
AhdI        Alw44I      AocI        ApaLI       ApoI        
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BamHI       BbeI        BbsI        BcgI        
BciVI       BfrI        BglII       BlnI        BpuAI       
BseAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BsrDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstXI       BstYI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KasI        MfeI        MflI        
MluI        MroI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Van91I      XbaI        
XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         157  GACGTC               GACGT^C
Acc65I       1024  GGTACC               G^GTACC
AccI         1417  GTCTAC               GT^[AC][GT]AC
AcyI          157  GACGTC               G[GA]^CG[CT]C
Aor51HI        57  AGCGCT               AGC^GCT
Asp700I       231  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1024  GGTACC               G^GTACC
BalI          543  TGGCCA               TGG^CCA
BbrPI         649  CACGTG               CAC^GTG
BclI          260  TGATCA               T^GATCA
BfaI          130  CTAG                 C^TAG
BpmI          898  CTCCAG               CTGGAG(16/14)
BsaAI         649  CACGTG               [CT]AC^GT[GA]
BsaHI         157  GACGTC               G[GA]^CG[CT]C
BsaWI         861  ACCGGA               [AT]^CCGG[AT]
BseRI          31  GAGGAG               GAGGAG(10/8)
BsmBI        1019  GAGACG               CGTCTC(1/5)
BspHI         821  TCATGA               T^CATGA
BspLU11I     1253  ACATGT               A^CATGT
BstAPI        948  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1155  GGTGACC              G^GT[ACGT]ACC
BstPI        1155  GGTGACC              G^GT[ACGT]ACC
DrdI         1260  GACTTCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          543  TGGCCA               [CT]^GGCC[GA]
Eco47III       57  AGCGCT               AGC^GCT
EcoNI         463  CCTGCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1155  GGTGACC              G^GT[ACGT]ACC
FbaI          260  TGATCA               T^GATCA
Hin1I         157  GACGTC               G[GA]^CG[CT]C
KpnI         1024  GGTACC               GGTAC^C
MaeI          130  CTAG                 C^TAG
MluNI         543  TGGCCA               TGG^CCA
MscI          543  TGGCCA               TGG^CCA
MseI          596  TTAA                 T^TAA
PleI          985  GACTC                GAGTC(4/5)
PmaCI         649  CACGTG               CAC^GTG
PmlI          649  CACGTG               CAC^GTG
RcaI          821  TCATGA               T^CATGA
SfiI           36  GGCCTTCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          987  CTCAAG               C^T[CT][GA]AG
StuI           35  AGGCCT               AGG^CCT
TaqI          191  TCGA                 T^CGA
Tru9I         596  TTAA                 T^TAA
Tsp509I       717  AATT                 ^AATT
TthHB8I       191  TCGA                 T^CGA
XcmI          857  CCACACCGGACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          231  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII        650  ACGTGT               A^C[GA][CT]GT
             1253  ACATGT              
AlwI         1055  GATCC                GGATC(4/5)
             1089  GGATC               
AlwNI         505  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              949  CAGGACCTG           
AspHI         223  GAGCTC               G[AT]GC[AT]^C
             1383  GAGCTC              
AspI          157  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              352  GACATTGTC           
BbvCI         114  CCTCAGC              CCTCAGC(-5/-2)
              960  CCTCAGC             
BglI           37  GCCTTCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              432  GCCAGGAAGGC         
BsaBI         258  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              367  GATGACCATC          
BsaI          661  GAGACC               GGTCTC(1/5)
              495  GGTCTC              
BsgI          642  CTGCAC               GTGCAG(16/14)
             1291  GTGCAG              
BsiHKAI       223  GAGCTC               G[AT]GC[AT]^C
             1383  GAGCTC              
BsrBI        1376  CCGCTC               CCGCTC(-3/-3)
             1450  GAGCGG              
BssSI         241  CACGAG               CACGAG(-5/-1)
              913  CACGAG              
EarI          994  CTCTTC               CTCTTC(1/4)
             1074  CTCTTC              
EcoT14I       737  CCAAGG               C^C[AT][AT]GG
             1494  CCAAGG              
Ksp632I       994  CTCTTC               CTCTTC(1/4)
             1074  CTCTTC              
MamI          258  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              367  GATGACCATC          
NspI         1171  GCATGT               [GA]CATG^[CT]
             1253  ACATGT              
PflFI         157  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              352  GACATTGTC           
PpuMI         144  GGGACCC              [GA]G^G[AT]CC[CT]
              950  AGGACCT             
PvuII         490  CAGCTG               CAG^CTG
              709  CAGCTG              
SacI          223  GAGCTC               GAGCT^C
             1383  GAGCTC              
StyI          737  CCAAGG               C^C[AT][AT]GG
             1494  CCAAGG              
Tth111I       157  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              352  GACATTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        ApaI        
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BlpI        BmyI        Bpu1102I    BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        KspI        
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PstI        RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        TfiI        
TseI        Tsp45I      TspRI       XmaCI       XmaI        
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