Restriction map for pF1KB9740
 (Linear) (Six-base) MAPSORT of: KIBB9740.seq  Check: 5200  from: 1  to: 1551



KIBB9740.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     432    1428    1551 
   Size:        432     996     123

  Fragments arranged by size: 

                 996     432     123
 
AccI GT'mk_AC

Cuts at:      0    1214    1551 
   Size:       1214     337
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637    1551 
   Size:        637     914
 
AhdI GACnn_n'nnGTC

Cuts at:      0     129    1551 
   Size:        129    1422
 
AleI CACnn'nnGTG

Cuts at:      0     151     230     997    1249    1551 
   Size:        151      79     767     252     302

  Fragments arranged by size: 

                 767     302     252     151      79
 
AlwNI CAG_nnn'CTG

Cuts at:      0     135    1050    1515    1551 
   Size:        135     915     465      36

  Fragments arranged by size: 

                 915     465     135      36
 
ApaI G_GGCC'C

Cuts at:      0     334    1551 
   Size:        334    1217
 
ApaLI G'TGCA_C

Cuts at:      0    1520    1551 
   Size:       1520      31
 
AvaI C'yCGr_G

Cuts at:      0     118     311     453    1266    1404    1551 
   Size:        118     193     142     813     138     147

  Fragments arranged by size: 

                 813     193     147     142     138     118
 
BanI G'GyrC_C

Cuts at:      0     232     457     944    1345    1492    1551 
   Size:        232     225     487     401     147      59

  Fragments arranged by size: 

                 487     401     232     225     147      59
 
BanII G_rGCy'C

Cuts at:      0     334     807    1209    1551 
   Size:        334     473     402     342

  Fragments arranged by size: 

                 473     402     342     334
 
BbeI G_GCGC'C

Cuts at:      0     948    1349    1551 
   Size:        948     401     202

  Fragments arranged by size: 

                 948     401     202
 
BbvCI CC'TCA_GC

Cuts at:      0    1168    1551 
   Size:       1168     383
 
BglI GCCn_nnn'nGGC

Cuts at:      0     299     488    1551 
   Size:        299     189    1063

  Fragments arranged by size: 

                1063     299     189
 
BglII A'GATC_T

Cuts at:      0     653    1551 
   Size:        653     898
 
Bme1580I G_kGCm'C

Cuts at:      0     334     462     472    1495    1524    1551 
   Size:        334     128      10    1023      29      27

  Fragments arranged by size: 

                1023     334     128      29      27      10
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1351    1503    1551 
   Size:       1351     152      48

  Fragments arranged by size: 

                1351     152      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1100    1109    1551 
   Size:       1100       9     442

  Fragments arranged by size: 

                1100     442       9
 
Bpu10I CC'TnA_GC

Cuts at:      0    1168    1551 
   Size:       1168     383
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533    1551 
   Size:        533    1018
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1453    1551 
   Size:       1453      98
 
BsaAI yAC'GTr

Cuts at:      0     531    1551 
   Size:        531    1020
 
BsaHI Gr'CG_yC

Cuts at:      0     945    1346    1551 
   Size:        945     401     205

  Fragments arranged by size: 

                 945     401     205
 
BsaWI w'CCGG_w

Cuts at:      0     881    1470    1551 
   Size:        881     589      81

  Fragments arranged by size: 

                 881     589      81
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     771     850     853     916    1321    1491    1494    1551 
   Size:        771      79       3      63     405     170       3      57

  Fragments arranged by size: 

                 771     405     170      79      63      57       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     241     294     461     521    1031    1043    1174    1551 
   Size:        241      53     167      60     510      12     131     377

  Fragments arranged by size: 

                 510     377     241     167     131      60      53      12
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541    1066    1135    1551 
   Size:        541     525      69     416

  Fragments arranged by size: 

                 541     525     416      69
 
BsiEI CG_ry'CG

Cuts at:      0     927    1551 
   Size:        927     624
 
BsiHKAI G_wGCw'C

Cuts at:      0     677    1524    1551 
   Size:        677     847      27

  Fragments arranged by size: 

                 847     677      27
 
BsmI GAATG_Cn'

Cuts at:      0     540    1551 
   Size:        540    1011
 
Bsp1286I G_dGCh'C

Cuts at:      0     334     462     472     677     807    1209    1495    1524 
   Size:        334     128      10     205     130     402     286      29

Cuts at:   1524    1551 
   Size:         27

  Fragments arranged by size: 

                 402     334     286     205     130     128      29      27
                  10
 
BspEI T'CCGG_A

Cuts at:      0    1470    1551 
   Size:       1470      81
 
BspHI T'CATG_A

Cuts at:      0     665    1551 
   Size:        665     886
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     263     432    1428    1551 
   Size:        134     129     169     996     123

  Fragments arranged by size: 

                 996     169     134     129     123
 
BsrBI CCG'CTC

Cuts at:      0     447     776    1182    1551 
   Size:        447     329     406     369

  Fragments arranged by size: 

                 447     406     369     329
 
BsrFI r'CCGG_y

Cuts at:      0     926     941    1551 
   Size:        926      15     610

  Fragments arranged by size: 

                 926     610      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     794    1111    1158    1551 
   Size:        794     317      47     393

  Fragments arranged by size: 

                 794     393     317      47
 
BstBI TT'CG_AA

Cuts at:      0      42    1551 
   Size:         42    1509
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     999    1551 
   Size:        999     552
 
BstYI r'GATC_y

Cuts at:      0     653    1551 
   Size:        653     898
 
Bsu36I CC'TnA_GG

Cuts at:      0     899    1386    1551 
   Size:        899     487     165

  Fragments arranged by size: 

                 899     487     165
 
BtgI C'CryG_G

Cuts at:      0     288     371     608    1004    1196    1551 
   Size:        288      83     237     396     192     355

  Fragments arranged by size: 

                 396     355     288     237     192      83
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     119    1551 
   Size:        119    1432
 
BtsI GCAGTG_nn'

Cuts at:      0     472    1147    1551 
   Size:        472     675     404

  Fragments arranged by size: 

                 675     472     404
 
EaeI y'GGCC_r

Cuts at:      0     186     248     342     890    1001    1551 
   Size:        186      62      94     548     111     550

  Fragments arranged by size: 

                 550     548     186     111      94      62
 
EarI CTCTTCn'nnn_

Cuts at:      0     850    1551 
   Size:        850     701
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637    1100    1109    1551 
   Size:        637     463       9     442

  Fragments arranged by size: 

                 637     463     442       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1303    1551 
   Size:       1303     248
 
EcoO109I rG'GnC_Cy

Cuts at:      0     123     331     402     864     972    1382    1551 
   Size:        123     208      71     462     108     410     169

  Fragments arranged by size: 

                 462     410     208     169     123     108      71
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     693    1551 
   Size:        693     858
 
HaeII r_GCGC'y

Cuts at:      0     948    1349    1551 
   Size:        948     401     202

  Fragments arranged by size: 

                 948     401     202
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     115     147    1551 
   Size:          4      32      79      32    1404

  Fragments arranged by size: 

                1404      79      32      32       4
 
KasI G'GCGC_C

Cuts at:      0     944    1345    1551 
   Size:        944     401     206

  Fragments arranged by size: 

                 944     401     206
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     887     947    1551 
   Size:        887      60     604

  Fragments arranged by size: 

                 887     604      60
 
MscI TGG'CCA

Cuts at:      0     344     892    1551 
   Size:        344     548     659

  Fragments arranged by size: 

                 659     548     344
 
MslI CAynn'nnrTG

Cuts at:      0     151     230     997    1237    1249    1551 
   Size:        151      79     767     240      12     302

  Fragments arranged by size: 

                 767     302     240     151      79      12
 
MspA1I CmG'CkG

Cuts at:      0     596    1551 
   Size:        596     955
 
NaeI GCC'GGC

Cuts at:      0     943    1551 
   Size:        943     608
 
NarI GG'CG_CC

Cuts at:      0     945    1346    1551 
   Size:        945     401     205

  Fragments arranged by size: 

                 945     401     205
 
NcoI C'CATG_G

Cuts at:      0     288     608    1196    1551 
   Size:        288     320     588     355

  Fragments arranged by size: 

                 588     355     320     288
 
NgoMIV G'CCGG_C

Cuts at:      0     941    1551 
   Size:        941     610
 
NspI r_CATG'y

Cuts at:      0     262     321    1551 
   Size:        262      59    1230

  Fragments arranged by size: 

                1230     262      59
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1515    1551 
   Size:       1515      36
 
PmlI CAC'GTG

Cuts at:      0     531    1551 
   Size:        531    1020
 
PpuMI rG'GwC_Cy

Cuts at:      0     123     402     864    1551 
   Size:        123     279     462     687

  Fragments arranged by size: 

                 687     462     279     123
 
PspOMI G'GGCC_C

Cuts at:      0     330    1551 
   Size:        330    1221
 
PstI C_TGCA'G

Cuts at:      0     131     173     401    1070    1292    1551 
   Size:        131      42     228     669     222     259

  Fragments arranged by size: 

                 669     259     228     222     131      42
 
PvuII CAG'CTG

Cuts at:      0     596    1551 
   Size:        596     955
 
SbfI CC_TGCA'GG

Cuts at:      0     401    1551 
   Size:        401    1150
 
SexAI A'CCwGG_T

Cuts at:      0    1088    1551 
   Size:       1088     463
 
SfcI C'TryA_G

Cuts at:      0     127     169     397     657    1066    1211    1288    1538 
   Size:        127      42     228     260     409     145      77     250

Cuts at:   1538    1551 
   Size:         13

  Fragments arranged by size: 

                 409     260     250     228     145     127      77      42
                  13
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     488    1551 
   Size:        488    1063
 
SfoI GGC'GCC

Cuts at:      0     946    1347    1551 
   Size:        946     401     204

  Fragments arranged by size: 

                 946     401     204
 
SmaI CCC'GGG

Cuts at:      0     120     455    1551 
   Size:        120     335    1096

  Fragments arranged by size: 

                1096     335     120
 
SmlI C'TyrA_G

Cuts at:      0     548    1551 
   Size:        548    1003
 
SphI G_CATG'C

Cuts at:      0     321    1551 
   Size:        321    1230
 
StuI AGG'CCT

Cuts at:      0    1391    1551 
   Size:       1391     160
 
StyI C'CwwG_G

Cuts at:      0     236     288     608    1196    1551 
   Size:        236      52     320     588     355

  Fragments arranged by size: 

                 588     355     320     236      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     190     242     445    1507    1551 
   Size:        190      52     203    1062      44

  Fragments arranged by size: 

                1062     203     190      52      44
 
TatI w'GTAC_w

Cuts at:      0     416    1551 
   Size:        416    1135
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     261     293    1551 
   Size:        261      32    1258

  Fragments arranged by size: 

                1258     261      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1059    1551 
   Size:       1059     492
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     388    1002    1551 
   Size:        388     614     549

  Fragments arranged by size: 

                 614     549     388
 
XmaI C'CCGG_G

Cuts at:      0     118     453    1551 
   Size:        118     335    1098

  Fragments arranged by size: 

                1098     335     118
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     AleI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbeI    BbvCI     BglI    BglII Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI   BstAPI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI    PflMI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SbfI    SexAI     SfcI     SfiI
     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AlfI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI     BmtI
     BplI    BsaBI    BsaXI    BsiWI    BsmBI    BsrDI    BsrGI   BssHII
    BssSI   BstEII  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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