Restriction map for pF1KB9727
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstNI       BstPI       BstUI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRII      EcoRV       FauI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        KspI        MaeII       MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvaI        MvnI        NaeI        NarI        NgoMIV      
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Asp700I       784  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         968  GAGCAC               G[AT]GC[AT]^C
BanI          698  GGCACC               G^G[CT][GA]CC
BanII         553  GAGCCC               G[GA]GC[CT]^C
BbsI          739  GAAGAC               GAAGAC(2/6)
BclI          110  TGATCA               T^GATCA
BglII         786  AGATCT               A^GATCT
BpuAI         739  GAAGAC               GAAGAC(2/6)
BsaBI         787  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         133  GAGGAG               GAGGAG(10/8)
BsiHKAI       968  GAGCAC               G[AT]GC[AT]^C
BsmFI        1287  GGGAC                GGGAC(10/14)
BspMI         691  GCAGGT               ACCTGC(4/8)
BssSI         982  CACGAG               CACGAG(-5/-1)
BstAPI        664  GCAACTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1181  CCATTGTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         786  AGATCT               [GA]^GATC[CT]
DraI          195  TTTAAA               TTT^AAA
DsaI         1004  CCATGG               C^C[GA][CT]GG
EcoNI         439  CCTATGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       563  ATGCAT               ATGCA^T
FbaI          110  TGATCA               T^GATCA
HgaI          907  GACGC                GACGC(5/10)
MamI          787  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          786  AGATCT               [GA]^GATC[CT]
NcoI         1004  CCATGG               C^CATGG
NdeI          866  CATATG               CA^TATG
NheI          682  GCTAGC               G^CTAGC
NlaIV         698  GGCACC               GG[ACGT]^[ACGT]CC
NsiI          563  ATGCAT               ATGCA^T
PstI          242  CTGCAG               CTGCA^G
ScaI          400  AGTACT               AGT^ACT
SfaNI         514  GATGC                GCATC(5/9)
SphI           87  GCATGC               GCATG^C
StuI          829  AGGCCT               AGG^CCT
XhoII         786  AGATCT               [GA]^GATC[CT]
XmnI          784  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AlwI           63  GATCC                GGATC(4/5)
              436  GATCC               
BsaI          343  GAGACC               GGTCTC(1/5)
             1069  GAGACC              
DraII         726  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1304  AGGACCT             
EaeI         1197  TGGCCG               [CT]^GGCC[GA]
             1231  CGGCCA              
EcoO109I      726  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1304  AGGACCT             
MaeIII       1036  GTGAC                ^GT[ACGT]AC
             1055  GTGAC               
NspI           87  GCATGC               [GA]CATG^[CT]
             1295  GCATGT              
PleI         1057  GACTC                GAGTC(4/5)
             1078  GAGTC               
PpuMI         726  AGGTCCT              [GA]G^G[AT]CC[CT]
             1304  AGGACCT             
PvuII        1025  CAGCTG               CAG^CTG
             1109  CAGCTG              
SmlI          154  CTCAAG               C^T[CT][GA]AG
              610  CTTGAG              
Tsp45I       1036  GTGAC                ^GT[GC]AC
             1055  GTGAC               
XcmI          858  CCAAGGGGCATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1181  CCATTGTCATGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      Cac8I       Cfr13I      
DdeI        DpnII       DrdI        EarI        EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        Ksp632I     MaeI        MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MwoI        NciI        NdeII       NlaIII      
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     
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