Restriction map for pF1KB9727
 (Linear) (Six-base) MAPSORT of: KIBB9727.seq  Check: 9710  from: 1  to: 1317



KIBB9727.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     682    1317 
   Size:        682     635
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     896    1317 
   Size:         44     852     421

  Fragments arranged by size: 

                 852     421      44
 
BanI G'GyrC_C

Cuts at:      0     698    1317 
   Size:        698     619
 
BanII G_rGCy'C

Cuts at:      0     557    1317 
   Size:        557     760
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     746    1317 
   Size:        746     571
 
BclI T'GATC_A

Cuts at:      0     110    1317 
   Size:        110    1207
 
BfrBI ATG'CAT

Cuts at:      0     565    1317 
   Size:        565     752
 
BglII A'GATC_T

Cuts at:      0     786    1317 
   Size:        786     531
 
Bme1580I G_kGCm'C

Cuts at:      0     701    1317 
   Size:        701     616
 
BmtI G_CTAG'C

Cuts at:      0     686    1317 
   Size:        686     631
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     991    1317 
   Size:        991     326
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     695    1016    1317 
   Size:        166     529     321     301

  Fragments arranged by size: 

                 529     321     301     166
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     631    1317 
   Size:        139     492     686

  Fragments arranged by size: 

                 686     492     139
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     337    1063    1317 
   Size:        337     726     254

  Fragments arranged by size: 

                 726     337     254
 
BsaBI GATnn'nnATC

Cuts at:      0     791    1317 
   Size:        791     526
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148    1317 
   Size:        148    1169
 
BseYI C'CCAG_C

Cuts at:      0     633     937    1317 
   Size:        633     304     380

  Fragments arranged by size: 

                 633     380     304
 
BsiHKAI G_wGCw'C

Cuts at:      0     972    1317 
   Size:        972     345
 
Bsp1286I G_dGCh'C

Cuts at:      0     557     701     972    1317 
   Size:        557     144     271     345

  Fragments arranged by size: 

                 557     345     271     144
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     682    1317 
   Size:        682     635
 
BsrDI GCAATG_nn'

Cuts at:      0     506     565    1289    1317 
   Size:        506      59     724      28

  Fragments arranged by size: 

                 724     506      59      28
 
BssSI C'ACGA_G

Cuts at:      0     982    1317 
   Size:        982     335
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     670    1317 
   Size:        670     647
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1188    1317 
   Size:       1188     129
 
BstYI r'GATC_y

Cuts at:      0     786    1317 
   Size:        786     531
 
BtgI C'CryG_G

Cuts at:      0    1004    1317 
   Size:       1004     313
 
BtsI GCAGTG_nn'

Cuts at:      0     970    1059    1317 
   Size:        970      89     258

  Fragments arranged by size: 

                 970     258      89
 
DraI TTT'AAA

Cuts at:      0     197    1317 
   Size:        197    1120
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      13    1077    1183    1317 
   Size:         13    1064     106     134

  Fragments arranged by size: 

                1064     134     106      13
 
EaeI y'GGCC_r

Cuts at:      0    1197    1231    1317 
   Size:       1197      34      86

  Fragments arranged by size: 

                1197      86      34
 
EarI CTCTTCn'nnn_

Cuts at:      0     479     950    1127    1136    1317 
   Size:        479     471     177       9     181

  Fragments arranged by size: 

                 479     471     181     177       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     166     695     896    1016    1317 
   Size:         44     122     529     201     120     301

  Fragments arranged by size: 

                 529     301     201     122     120      44
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     443    1317 
   Size:        443     874
 
EcoO109I rG'GnC_Cy

Cuts at:      0     727    1305    1317 
   Size:        727     578      12

  Fragments arranged by size: 

                 727     578      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     805    1317 
   Size:        805     512
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     478     510    1317 
   Size:        478      32     807

  Fragments arranged by size: 

                 807     478      32
 
MslI CAynn'nnrTG

Cuts at:      0     769     901    1186    1317 
   Size:        769     132     285     131

  Fragments arranged by size: 

                 769     285     132     131
 
MspA1I CmG'CkG

Cuts at:      0     458    1027    1042    1111    1317 
   Size:        458     569      15      69     206

  Fragments arranged by size: 

                 569     458     206      69      15
 
NcoI C'CATG_G

Cuts at:      0    1004    1317 
   Size:       1004     313
 
NdeI CA'TA_TG

Cuts at:      0     867    1317 
   Size:        867     450
 
NheI G'CTAG_C

Cuts at:      0     682    1317 
   Size:        682     635
 
NsiI A_TGCA'T

Cuts at:      0     567    1317 
   Size:        567     750
 
NspI r_CATG'y

Cuts at:      0      91    1299    1317 
   Size:         91    1208      18

  Fragments arranged by size: 

                1208      91      18
 
PpuMI rG'GwC_Cy

Cuts at:      0     727    1305    1317 
   Size:        727     578      12

  Fragments arranged by size: 

                 727     578      12
 
PstI C_TGCA'G

Cuts at:      0     246    1317 
   Size:        246    1071
 
PvuII CAG'CTG

Cuts at:      0    1027    1111    1317 
   Size:       1027      84     206

  Fragments arranged by size: 

                1027     206      84
 
ScaI AGT'ACT

Cuts at:      0     402    1317 
   Size:        402     915
 
SfcI C'TryA_G

Cuts at:      0     242     393    1212    1317 
   Size:        242     151     819     105

  Fragments arranged by size: 

                 819     242     151     105
 
SmlI C'TyrA_G

Cuts at:      0     154     610    1317 
   Size:        154     456     707

  Fragments arranged by size: 

                 707     456     154
 
SphI G_CATG'C

Cuts at:      0      91    1317 
   Size:         91    1226
 
StuI AGG'CCT

Cuts at:      0     831    1317 
   Size:        831     486
 
StyI C'CwwG_G

Cuts at:      0      92      98     858    1004    1317 
   Size:         92       6     760     146     313

  Fragments arranged by size: 

                 760     313     146      92       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     361    1317 
   Size:        361     956
 
TatI w'GTAC_w

Cuts at:      0     400     460    1317 
   Size:        400      60     857

  Fragments arranged by size: 

                 857     400      60
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      12      44     108     140     711     743    1317 
   Size:         12      32      64      32     571      32     574

  Fragments arranged by size: 

                 574     571      64      32      32      32      12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     865    1188    1317 
   Size:        865     323     129

  Fragments arranged by size: 

                 865     323     129
 
XmnI GAAnn'nnTTC

Cuts at:      0     788    1317 
   Size:        788     529
 

 Enzymes that do cut:

     AarI     AcuI     BanI    BanII     BbsI     BclI    BfrBI    BglII
 Bme1580I     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspMI    BsrDI    BssSI   BstAPI    BstXI
    BstYI     BtgI     BtsI     DraI     DrdI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I     FalI    Hin4I     MslI   MspA1I     NcoI     NdeI
     NheI     NsiI     NspI    PpuMI     PstI    PvuII     ScaI     SfcI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BglI     BlpI    BmgBI     BmrI   Bpu10I
    BsaAI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI   NgoMIV     NotI     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI
     XhoI     XmaI     ZraI
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