Restriction map for pF1KB9726
 (Linear) (Six-base) MAPSORT of: KIBB9726.seq  Check: 8117  from: 1  to: 1293



KIBB9726.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AfeI AGC'GCT

Cuts at:      0     145    1293 
   Size:        145    1148
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     601     633    1293 
   Size:        601      32     660

  Fragments arranged by size: 

                 660     601      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     168    1293 
   Size:        168    1125
 
ApaI G_GGCC'C

Cuts at:      0     326     775    1293 
   Size:        326     449     518

  Fragments arranged by size: 

                 518     449     326
 
AvaI C'yCGr_G

Cuts at:      0     238     546     979    1293 
   Size:        238     308     433     314

  Fragments arranged by size: 

                 433     314     308     238
 
BamHI G'GATC_C

Cuts at:      0    1077    1293 
   Size:       1077     216
 
BanI G'GyrC_C

Cuts at:      0     358     946     975    1015    1164    1176    1293 
   Size:        358     588      29      40     149      12     117

  Fragments arranged by size: 

                 588     358     149     117      40      29      12
 
BanII G_rGCy'C

Cuts at:      0     206     245     326     544     775     865    1293 
   Size:        206      39      81     218     231      90     428

  Fragments arranged by size: 

                 428     231     218     206      90      81      39
 
BbeI G_GCGC'C

Cuts at:      0     950     979    1019    1168    1180    1293 
   Size:        950      29      40     149      12     113

  Fragments arranged by size: 

                 950     149     113      40      29      12
 
BbvCI CC'TCA_GC

Cuts at:      0     679    1293 
   Size:        679     614
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      60      94    1293 
   Size:         60      34    1199

  Fragments arranged by size: 

                1199      60      34
 
BglII A'GATC_T

Cuts at:      0     698    1293 
   Size:        698     595
 
BlpI GC'TnA_GC

Cuts at:      0     147    1293 
   Size:        147    1146
 
Bme1580I G_kGCm'C

Cuts at:      0     326     775    1293 
   Size:        326     449     518

  Fragments arranged by size: 

                 518     449     326
 
BmgBI CAC'GTC

Cuts at:      0     996    1293 
   Size:        996     297
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     269    1293 
   Size:        269    1024
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     628     764    1293 
   Size:        148     480     136     529

  Fragments arranged by size: 

                 529     480     148     136
 
Bpu10I CC'TnA_GC

Cuts at:      0     679    1293 
   Size:        679     614
 
BsaAI yAC'GTr

Cuts at:      0    1056    1293 
   Size:       1056     237
 
BsaBI GATnn'nnATC

Cuts at:      0     697    1293 
   Size:        697     596
 
BsaHI Gr'CG_yC

Cuts at:      0     559     947     976    1016    1027    1165    1177    1293 
   Size:        559     388      29      40      11     138      12     116

  Fragments arranged by size: 

                 559     388     138     116      40      29      12      11
            
 
BsaWI w'CCGG_w

Cuts at:      0    1047    1293 
   Size:       1047     246
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     601     631    1293 
   Size:        601      30     662

  Fragments arranged by size: 

                 662     601      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1221    1238    1241    1293 
   Size:       1221      17       3      52

  Fragments arranged by size: 

                1221      52      17       3
 
BseYI C'CCAG_C

Cuts at:      0     325     850    1180    1293 
   Size:        325     525     330     113

  Fragments arranged by size: 

                 525     330     325     113
 
BsiEI CG_ry'CG

Cuts at:      0     818    1293 
   Size:        818     475
 
BsiHKAI G_wGCw'C

Cuts at:      0     206    1293 
   Size:        206    1087
 
BsmI GAATG_Cn'

Cuts at:      0     903    1293 
   Size:        903     390
 
Bsp1286I G_dGCh'C

Cuts at:      0     206     245     326     544     775     865    1293 
   Size:        206      39      81     218     231      90     428

  Fragments arranged by size: 

                 428     231     218     206      90      81      39
 
BspHI T'CATG_A

Cuts at:      0     824    1293 
   Size:        824     469
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     677    1293 
   Size:        677     616
 
BsrBI CCG'CTC

Cuts at:      0     687    1293 
   Size:        687     606
 
BsrDI GCAATG_nn'

Cuts at:      0      78    1293 
   Size:         78    1215
 
BsrFI r'CCGG_y

Cuts at:      0     641     713     784     817    1096    1293 
   Size:        641      72      71      33     279     197

  Fragments arranged by size: 

                 641     279     197      72      71      33
 
BssHII G'CGCG_C

Cuts at:      0     590    1293 
   Size:        590     703
 
BssSI C'ACGA_G

Cuts at:      0     585    1293 
   Size:        585     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     804    1293 
   Size:        804     489
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     126     753    1293 
   Size:        126     627     540

  Fragments arranged by size: 

                 627     540     126
 
BstYI r'GATC_y

Cuts at:      0     698    1077    1293 
   Size:        698     379     216

  Fragments arranged by size: 

                 698     379     216
 
Bsu36I CC'TnA_GG

Cuts at:      0    1253    1293 
   Size:       1253      40
 
BtgI C'CryG_G

Cuts at:      0      29     812    1191    1293 
   Size:         29     783     379     102

  Fragments arranged by size: 

                 783     379     102      29
 
BtsI GCAGTG_nn'

Cuts at:      0     498    1293 
   Size:        498     795
 
EaeI y'GGCC_r

Cuts at:      0     662     815    1293 
   Size:        662     153     478

  Fragments arranged by size: 

                 662     478     153
 
EagI C'GGCC_G

Cuts at:      0     815    1293 
   Size:        815     478
 
EarI CTCTTCn'nnn_

Cuts at:      0      46    1293 
   Size:         46    1247
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     500    1293 
   Size:        500     793
 
EcoICRI GAG'CTC

Cuts at:      0     204    1293 
   Size:        204    1089
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     628     764    1293 
   Size:        148     480     136     529

  Fragments arranged by size: 

                 529     480     148     136
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     935    1293 
   Size:        935     358
 
EcoO109I rG'GnC_Cy

Cuts at:      0     771    1293 
   Size:        771     522
 
HaeII r_GCGC'y

Cuts at:      0     147     950     979    1019    1168    1180    1276    1293 
   Size:        147     803      29      40     149      12      96      17

  Fragments arranged by size: 

                 803     149     147      96      40      29      17      12
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     213     232     245     264     850     882    1216    1248 
   Size:        213      19      13      19     586      32     334      32

Cuts at:   1248    1293 
   Size:         45

  Fragments arranged by size: 

                 586     334     213      45      32      32      19      19
                  13
 
KasI G'GCGC_C

Cuts at:      0     946     975    1015    1164    1176    1293 
   Size:        946      29      40     149      12     117

  Fragments arranged by size: 

                 946     149     117      40      29      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     941     991    1293 
   Size:        941      50     302

  Fragments arranged by size: 

                 941     302      50
 
MscI TGG'CCA

Cuts at:      0     664    1293 
   Size:        664     629
 
MslI CAynn'nnrTG

Cuts at:      0     896    1293 
   Size:        896     397
 
MspA1I CmG'CkG

Cuts at:      0      31     215     503     597    1083    1293 
   Size:         31     184     288      94     486     210

  Fragments arranged by size: 

                 486     288     210     184      94      31
 
NaeI GCC'GGC

Cuts at:      0     643     786     819    1098    1293 
   Size:        643     143      33     279     195

  Fragments arranged by size: 

                 643     279     195     143      33
 
NarI GG'CG_CC

Cuts at:      0     947     976    1016    1165    1177    1293 
   Size:        947      29      40     149      12     116

  Fragments arranged by size: 

                 947     149     116      40      29      12
 
NgoMIV G'CCGG_C

Cuts at:      0     641     784     817    1096    1293 
   Size:        641     143      33     279     197

  Fragments arranged by size: 

                 641     279     197     143      33
 
NspI r_CATG'y

Cuts at:      0     190    1293 
   Size:        190    1103
 
PasI CC'CwG_GG

Cuts at:      0     168    1293 
   Size:        168    1125
 
PfoI T'CCnGG_A

Cuts at:      0    1141    1293 
   Size:       1141     152
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     601     633    1293 
   Size:        601      32     660

  Fragments arranged by size: 

                 660     601      32
 
PspOMI G'GGCC_C

Cuts at:      0     322     771    1293 
   Size:        322     449     522

  Fragments arranged by size: 

                 522     449     322
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1138    1170    1293 
   Size:       1138      32     123

  Fragments arranged by size: 

                1138     123      32
 
PstI C_TGCA'G

Cuts at:      0     164    1293 
   Size:        164    1129
 
PvuII CAG'CTG

Cuts at:      0     215     597    1293 
   Size:        215     382     696

  Fragments arranged by size: 

                 696     382     215
 
SacI G_AGCT'C

Cuts at:      0     206    1293 
   Size:        206    1087
 
SacII CC_GC'GG

Cuts at:      0      32    1293 
   Size:         32    1261
 
SfcI C'TryA_G

Cuts at:      0     160    1293 
   Size:        160    1133
 
SfoI GGC'GCC

Cuts at:      0     948     977    1017    1166    1178    1293 
   Size:        948      29      40     149      12     115

  Fragments arranged by size: 

                 948     149     115      40      29      12
 
SmaI CCC'GGG

Cuts at:      0     548    1293 
   Size:        548     745
 
StyI C'CwwG_G

Cuts at:      0     746    1293 
   Size:        746     547
 
TatI w'GTAC_w

Cuts at:      0     725    1293 
   Size:        725     568
 
XmaI C'CCGG_G

Cuts at:      0     546    1293 
   Size:        546     747
 
XmnI GAAnn'nnTTC

Cuts at:      0     616    1293 
   Size:        616     677
 

 Enzymes that do cut:

     AfeI     AloI    AlwNI     ApaI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PfoI     PpiI
   PspOMI     PsrI     PstI    PvuII     SacI    SacII     SfcI     SfoI
     SmaI     StyI     TatI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbsI    BciVI     BclI    BfrBI     BglI
     BmtI     BplI    BpuEI     BsaI     BsgI    BsiWI    BsmBI    BspEI
    BsrGI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI
     PmlI    PpuMI    PshAI     PsiI    PspXI     PvuI    RsrII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
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