Restriction map for pF1KB9694
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AfaI        
AflII       AflIII      AhdI        AocI        ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BanII       BbrPI       BbsI        BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsgI        
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
Eam1105I    EarI        Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     MamI        MfeI        MluI        MslI        
MunI        NcoI        NdeI        NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI          187  GTCTAC               GT^[AC][GT]AC
AccIII        726  TCCGGA               T^CCGGA
AgeI          695  ACCGGT               A^CCGGT
Alw44I        253  GTGCAC               G^TGCAC
AlwNI         634  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        20  AGCGCT               AGC^GCT
ApaLI         253  GTGCAC               G^TGCAC
AspHI         253  GTGCAC               G[AT]GC[AT]^C
AvaI          421  CCCGAG               C^[CT]CG[GA]G
AvaII          84  GGACC                G^G[AT]CC
BalI           47  TGGCCA               TGG^CCA
BbvCI         570  CCTCAGC              CCTCAGC(-5/-2)
BfaI          416  CTAG                 C^TAG
BmyI          253  GTGCAC               G[AGT]GC[ACT]^C
BsaAI         532  TACGTG               [CT]AC^GT[GA]
BsaI          222  GGTCTC               GGTCTC(1/5)
BseAI         726  TCCGGA               T^CCGGA
BseRI         300  CTCCTC               GAGGAG(10/8)
BsiEI         158  CGGCCG               CG[GA][CT]^CG
BsiHKAI       253  GTGCAC               G[AT]GC[AT]^C
BsmAI         223  GTCTC                GTCTC(1/5)
BsoBI         421  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      253  GTGCAC               G[AGT]GC[ACT]^C
BspEI         726  TCCGGA               T^CCGGA
BsrI          250  CCAGT                ACTGG(1/-1)
BstAPI        255  GCACCCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII          83  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI           98  CCGCGG               C^C[GA][CT]GG
EagI          158  CGGCCG               C^GGCCG
EclXI         158  CGGCCG               C^GGCCG
Eco47III       20  AGCGCT               AGC^GCT
Eco52I        158  CGGCCG               C^GGCCG
EcoO109I       83  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV         383  GATATC               GAT^ATC
HphI           29  TCACC                GGTGA(8/7)
KspI           98  CCGCGG               CCGC^GG
MaeI          416  CTAG                 C^TAG
MaeIII        742  GTGAC                ^GT[ACGT]AC
MluNI          47  TGGCCA               TGG^CCA
MroI          726  TCCGGA               T^CCGGA
MscI           47  TGGCCA               TGG^CCA
MseI          692  TTAA                 T^TAA
NaeI            9  GCCGGC               GCC^GGC
NgoMIV          9  GCCGGC               G^CCGGC
PinAI         695  ACCGGT               A^CCGGT
PpuMI          83  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII         556  CAGCTG               CAG^CTG
SacII          98  CCGCGG               CCGC^GG
TaqI           35  TCGA                 T^CGA
Tru9I         692  TTAA                 T^TAA
Tsp45I        742  GTGAC                ^GT[GC]AC
TspRI         251  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        35  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

BamHI         342  GGATCC               G^GATCC
              467  GGATCC              
BbeI           12  GGCGCC               GGCGC^C
              541  GGCGCC              
BcnI          198  CCCGG                CC^[GC]GG
              258  CCCGG               
BpmI          120  CTCCAG               CTGGAG(16/14)
              640  CTGGAG              
BsaWI         695  ACCGGT               [AT]^CCGG[AT]
              726  TCCGGA              
BstF5I         90  CATCC                GGATG(2/0)
              369  CATCC               
BstNI         279  CCTGG                CC^[AT]GG
              362  CCTGG               
BstYI         342  GGATCC               [GA]^GATC[CT]
              467  GGATCC              
Cfr13I         84  GGACC                G^G[ACGT]CC
              450  GGCCC               
DpnII         343  GATC                 ^GATC
              468  GATC                
DraIII        256  CACCCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              529  CACTACGTG           
EcoRII        279  CCTGG                ^CC[AT]GG
              362  CCTGG               
EcoT14I        50  CCAAGG               C^C[AT][AT]GG
              402  CCAAGG              
FokI           90  CATCC                GGATG(9/13)
              369  CATCC               
HgaI          681  GACGC                GACGC(5/10)
              185  GCGTC               
KasI           12  GGCGCC               G^GCGCC
              541  GGCGCC              
MaeII         533  ACGT                 A^CGT
              740  ACGT                
MboI          343  GATC                 ^GATC
              468  GATC                
MflI          342  GGATCC               [GA]^GATC[CT]
              467  GGATCC              
MvaI          279  CCTGG                CC^[AT]GG
              362  CCTGG               
NarI           12  GGCGCC               GG^CGCC
              541  GGCGCC              
NciI          198  CCCGG                CC^[GC]GG
              258  CCCGG               
NdeII         343  GATC                 ^GATC
              468  GATC                
PleI           37  GAGTC                GAGTC(4/5)
              723  GAGTC               
Sau3AI        343  GATC                 ^GATC
              468  GATC                
Sau96I         84  GGACC                G^G[ACGT]CC
              450  GGCCC               
SfoI           12  GGCGCC               GGC^GCC
              541  GGCGCC              
StyI           50  CCAAGG               C^C[AT][AT]GG
              402  CCAAGG              
XcmI           32  CCATCGAGTCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              194  CCAACCCGGACTTGG     
XhoII         342  GGATCC               [GA]^GATC[CT]
              467  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
BanI        BbvI        BsaHI       BsaJI       BsiYI       
BslI        BsmFI       BsrFI       BssKI       BstUI       
Cac8I       CfoI        Cfr10I      DdeI        EaeI        
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
ItaI        MboII       MnlI        MspA1I      MspI        
MvnI        MwoI        NlaIV       ScrFI       TfiI        
TseI        
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