Restriction map for pF1KB9694
 (Linear) (Six-base) MAPSORT of: KIBB9694.seq  Check: 6884  from: 1  to: 756



KIBB9694.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AccI GT'mk_AC

Cuts at:      0     188     756 
   Size:        188     568
 
AfeI AGC'GCT

Cuts at:      0      22     756 
   Size:         22     734
 
AgeI A'CCGG_T

Cuts at:      0     695     756 
   Size:        695      61
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     193     225     756 
   Size:        193      32     531

  Fragments arranged by size: 

                 531     193      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     639     756 
   Size:        639     117
 
ApaLI G'TGCA_C

Cuts at:      0     253     756 
   Size:        253     503
 
AvaI C'yCGr_G

Cuts at:      0     421     756 
   Size:        421     335
 
BamHI G'GATC_C

Cuts at:      0     342     467     756 
   Size:        342     125     289

  Fragments arranged by size: 

                 342     289     125
 
BanI G'GyrC_C

Cuts at:      0      12     261     541     756 
   Size:         12     249     280     215

  Fragments arranged by size: 

                 280     249     215      12
 
BbeI G_GCGC'C

Cuts at:      0      16     545     756 
   Size:         16     529     211

  Fragments arranged by size: 

                 529     211      16
 
BbvCI CC'TCA_GC

Cuts at:      0     571     756 
   Size:        571     185
 
Bme1580I G_kGCm'C

Cuts at:      0     257     756 
   Size:        257     499
 
BmgBI CAC'GTC

Cuts at:      0     741     756 
   Size:        741      15
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     240     756 
   Size:        240     516
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     661     756 
   Size:        105     556      95

  Fragments arranged by size: 

                 556     105      95
 
Bpu10I CC'TnA_GC

Cuts at:      0     571     756 
   Size:        571     185
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     228     756 
   Size:        228     528
 
BsaAI yAC'GTr

Cuts at:      0     534     756 
   Size:        534     222
 
BsaHI Gr'CG_yC

Cuts at:      0      13     185     542     756 
   Size:         13     172     357     214

  Fragments arranged by size: 

                 357     214     172      13
 
BsaWI w'CCGG_w

Cuts at:      0     695     726     756 
   Size:        695      31      30

  Fragments arranged by size: 

                 695      31      30
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     103     133     756 
   Size:        103      30     623

  Fragments arranged by size: 

                 623     103      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     291     756 
   Size:        291     465
 
BseYI C'CCAG_C

Cuts at:      0     491     756 
   Size:        491     265
 
BsiEI CG_ry'CG

Cuts at:      0     161     756 
   Size:        161     595
 
BsiHKAI G_wGCw'C

Cuts at:      0     257     756 
   Size:        257     499
 
Bsp1286I G_dGCh'C

Cuts at:      0     257     756 
   Size:        257     499
 
BspEI T'CCGG_A

Cuts at:      0     726     756 
   Size:        726      30
 
BsrFI r'CCGG_y

Cuts at:      0       9     175     695     756 
   Size:          9     166     520      61

  Fragments arranged by size: 

                 520     166      61       9
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     261     756 
   Size:        261     495
 
BstYI r'GATC_y

Cuts at:      0     342     467     756 
   Size:        342     125     289

  Fragments arranged by size: 

                 342     289     125
 
BtgI C'CryG_G

Cuts at:      0      98     756 
   Size:         98     658
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     379     756 
   Size:        379     377
 
DraIII CAC_nnn'GTG

Cuts at:      0     261     534     756 
   Size:        261     273     222

  Fragments arranged by size: 

                 273     261     222
 
EaeI y'GGCC_r

Cuts at:      0      47     158     281     756 
   Size:         47     111     123     475

  Fragments arranged by size: 

                 475     123     111      47
 
EagI C'GGCC_G

Cuts at:      0     158     756 
   Size:        158     598
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     661     756 
   Size:        105     556      95

  Fragments arranged by size: 

                 556     105      95
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84     756 
   Size:         84     672
 
EcoRV GAT'ATC

Cuts at:      0     385     756 
   Size:        385     371
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      42     756 
   Size:         42     714
 
HaeII r_GCGC'y

Cuts at:      0      16      24     545     756 
   Size:         16       8     521     211

  Fragments arranged by size: 

                 521     211      16       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     730     756 
   Size:        730      26
 
KasI G'GCGC_C

Cuts at:      0      12     541     756 
   Size:         12     529     215

  Fragments arranged by size: 

                 529     215      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     756 
   Size:        218     538
 
MscI TGG'CCA

Cuts at:      0      49     756 
   Size:         49     707
 
MspA1I CmG'CkG

Cuts at:      0     100     339     558     756 
   Size:        100     239     219     198

  Fragments arranged by size: 

                 239     219     198     100
 
NaeI GCC'GGC

Cuts at:      0      11     756 
   Size:         11     745
 
NarI GG'CG_CC

Cuts at:      0      13     542     756 
   Size:         13     529     214

  Fragments arranged by size: 

                 529     214      13
 
NgoMIV G'CCGG_C

Cuts at:      0       9     756 
   Size:          9     747
 
PpuMI rG'GwC_Cy

Cuts at:      0      84     756 
   Size:         84     672
 
PvuII CAG'CTG

Cuts at:      0     558     756 
   Size:        558     198
 
SacII CC_GC'GG

Cuts at:      0     101     756 
   Size:        101     655
 
SfoI GGC'GCC

Cuts at:      0      14     543     756 
   Size:         14     529     213

  Fragments arranged by size: 

                 529     213      14
 
StyI C'CwwG_G

Cuts at:      0      50     402     756 
   Size:         50     352     354

  Fragments arranged by size: 

                 354     352      50
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      39     201     756 
   Size:         39     162     555

  Fragments arranged by size: 

                 555     162      39
 

 Enzymes that do cut:

     AccI     AfeI     AgeI     AloI    AlwNI    ApaLI     AvaI    BamHI
     BanI     BbeI    BbvCI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BsrFI   BstAPI    BstYI     BtgI    BtgZI
   DraIII     EaeI     EagI  Eco57MI EcoO109I    EcoRV     FalI    HaeII
    Hin4I     KasI     MmeI     MscI   MspA1I     NaeI     NarI   NgoMIV
    PpuMI    PvuII    SacII     SfoI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AhdI
     AleI     AlfI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BanII     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmrI     BmtI    BpuEI    BsaBI     BsgI    BsiWI
     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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