Restriction map for pF1KB9689
 (Linear) (Six-base) MAPSORT of: KIBB9689.seq  Check: 2916  from: 1  to: 690



KIBB9689.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

ApaLI G'TGCA_C

Cuts at:      0     505     690 
   Size:        505     185
 
AscI GG'CGCG_CC

Cuts at:      0     578     690 
   Size:        578     112
 
AvaI C'yCGr_G

Cuts at:      0       8     128     690 
   Size:          8     120     562

  Fragments arranged by size: 

                 562     120       8
 
BanI G'GyrC_C

Cuts at:      0      68     585     639     690 
   Size:         68     517      54      51

  Fragments arranged by size: 

                 517      68      54      51
 
BanII G_rGCy'C

Cuts at:      0     221     690 
   Size:        221     469
 
BbeI G_GCGC'C

Cuts at:      0      72     589     643     690 
   Size:         72     517      54      47

  Fragments arranged by size: 

                 517      72      54      47
 
BbvCI CC'TCA_GC

Cuts at:      0     103     690 
   Size:        103     587
 
BglI GCCn_nnn'nGGC

Cuts at:      0     232     349     469     690 
   Size:        232     117     120     221

  Fragments arranged by size: 

                 232     221     120     117
 
Bme1580I G_kGCm'C

Cuts at:      0     509     690 
   Size:        509     181
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     250     496     690 
   Size:        250     246     194

  Fragments arranged by size: 

                 250     246     194
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     178     221     419     690 
   Size:        139      39      43     198     271

  Fragments arranged by size: 

                 271     198     139      43      39
 
Bpu10I CC'TnA_GC

Cuts at:      0     103     690 
   Size:        103     587
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     216     690 
   Size:         31     185     474

  Fragments arranged by size: 

                 474     185      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     672     690 
   Size:        672      18
 
BsaHI Gr'CG_yC

Cuts at:      0      69     266     341     586     640     690 
   Size:         69     197      75     245      54      50

  Fragments arranged by size: 

                 245     197      75      69      54      50
 
BsaWI w'CCGG_w

Cuts at:      0     143     295     595     690 
   Size:        143     152     300      95

  Fragments arranged by size: 

                 300     152     143      95
 
BseYI C'CCAG_C

Cuts at:      0     362     690 
   Size:        362     328
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     472     690 
   Size:        364     108     218

  Fragments arranged by size: 

                 364     218     108
 
BsiEI CG_ry'CG

Cuts at:      0     227     353     690 
   Size:        227     126     337

  Fragments arranged by size: 

                 337     227     126
 
BsiHKAI G_wGCw'C

Cuts at:      0     509     690 
   Size:        509     181
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     509     690 
   Size:        221     288     181

  Fragments arranged by size: 

                 288     221     181
 
BspEI T'CCGG_A

Cuts at:      0     143     690 
   Size:        143     547
 
BsrBI CCG'CTC

Cuts at:      0     123     690 
   Size:        123     567
 
BsrFI r'CCGG_y

Cuts at:      0     417     468     582     690 
   Size:        417      51     114     108

  Fragments arranged by size: 

                 417     114     108      51
 
BssHII G'CGCG_C

Cuts at:      0     561     578     690 
   Size:        561      17     112

  Fragments arranged by size: 

                 561     112      17
 
BstEII G'GTnAC_C

Cuts at:      0       3     690 
   Size:          3     687
 
BstYI r'GATC_y

Cuts at:      0     423     690 
   Size:        423     267
 
BtgI C'CryG_G

Cuts at:      0     185     230     690 
   Size:        185      45     460

  Fragments arranged by size: 

                 460     185      45
 
EaeI y'GGCC_r

Cuts at:      0     224     350     690 
   Size:        224     126     340

  Fragments arranged by size: 

                 340     224     126
 
EagI C'GGCC_G

Cuts at:      0     224     350     690 
   Size:        224     126     340

  Fragments arranged by size: 

                 340     224     126
 
EarI CTCTTCn'nnn_

Cuts at:      0      92     373     690 
   Size:         92     281     317

  Fragments arranged by size: 

                 317     281      92
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     178     221     419     690 
   Size:        139      39      43     198     271

  Fragments arranged by size: 

                 271     198     139      43      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     648     690 
   Size:        648      42
 
FspI TGC'GCA

Cuts at:      0     534     690 
   Size:        534     156
 
HaeII r_GCGC'y

Cuts at:      0      72     277     442     498     589     643     690 
   Size:         72     205     165      56      91      54      47

  Fragments arranged by size: 

                 205     165      91      72      56      54      47
 
KasI G'GCGC_C

Cuts at:      0      68     585     639     690 
   Size:         68     517      54      51

  Fragments arranged by size: 

                 517      68      54      51
 
MspA1I CmG'CkG

Cuts at:      0     232     284     654     690 
   Size:        232      52     370      36

  Fragments arranged by size: 

                 370     232      52      36
 
NaeI GCC'GGC

Cuts at:      0     584     690 
   Size:        584     106
 
NarI GG'CG_CC

Cuts at:      0      69     586     640     690 
   Size:         69     517      54      50

  Fragments arranged by size: 

                 517      69      54      50
 
NgoMIV G'CCGG_C

Cuts at:      0     582     690 
   Size:        582     108
 
PfoI T'CCnGG_A

Cuts at:      0     260     690 
   Size:        260     430
 
PvuII CAG'CTG

Cuts at:      0     284     690 
   Size:        284     406
 
SacII CC_GC'GG

Cuts at:      0     233     690 
   Size:        233     457
 
SapI GCTCTTCn'nnn_

Cuts at:      0      92     373     690 
   Size:         92     281     317

  Fragments arranged by size: 

                 317     281      92
 
SfoI GGC'GCC

Cuts at:      0      70     587     641     690 
   Size:         70     517      54      49

  Fragments arranged by size: 

                 517      70      54      49
 
SgrAI Cr'CCGG_yG

Cuts at:      0     582     690 
   Size:        582     108
 
SmaI CCC'GGG

Cuts at:      0      10     130     690 
   Size:         10     120     560

  Fragments arranged by size: 

                 560     120      10
 
SmlI C'TyrA_G

Cuts at:      0      46     195     690 
   Size:         46     149     495

  Fragments arranged by size: 

                 495     149      46
 
StuI AGG'CCT

Cuts at:      0     432     690 
   Size:        432     258
 
StyI C'CwwG_G

Cuts at:      0     474     644     682     690 
   Size:        474     170      38       8

  Fragments arranged by size: 

                 474     170      38       8
 
XmaI C'CCGG_G

Cuts at:      0       8     128     690 
   Size:          8     120     562

  Fragments arranged by size: 

                 562     120       8
 

 Enzymes that do cut:

    ApaLI     AscI     AvaI     BanI    BanII     BbeI    BbvCI     BglI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BsrBI    BsrFI
   BssHII   BstEII    BstYI     BtgI     EaeI     EagI     EarI  Eco57MI
 EcoO109I     FspI    HaeII     KasI   MspA1I     NaeI     NarI   NgoMIV
     PfoI    PvuII    SacII     SapI     SfoI    SgrAI     SmaI     SmlI
     StuI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     ApoI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI
    BsaAI    BsaBI    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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