Restriction map for pF1KB9661
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AflII       AflIII      AgeI        AhdI        
AlwNI       AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BclI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AcsI          652  AAATTC               [GA]^AATT[CT]
AcyI          913  GGCGTC               G[GA]^CG[CT]C
ApaI          540  GGGCCC               GGGCC^C
ApoI          652  AAATTC               [GA]^AATT[CT]
AvaII         328  GGACC                G^G[AT]CC
BbvCI         429  GCTGAGG              CCTCAGC(-5/-2)
BciVI         498  GGATAC               GTATCC(6/5)
BfaI          949  CTAG                 C^TAG
BglII         452  AGATCT               A^GATCT
BpmI           17  CTCCAG               CTGGAG(16/14)
BsaAI         566  TACGTG               [CT]AC^GT[GA]
BsaHI         913  GGCGTC               G[GA]^CG[CT]C
BsaWI         165  ACCGGA               [AT]^CCGG[AT]
BseRI         424  GAGGAG               GAGGAG(10/8)
BsiEI         163  CGACCG               CG[GA][CT]^CG
BsmFI         327  GGGAC                GGGAC(10/14)
BsrDI         512  GCAATG               GCAATG(2/0)
BstEII        180  GGTGACC              G^GT[ACGT]ACC
BstPI         180  GGTGACC              G^GT[ACGT]ACC
DdeI          430  CTGAG                C^T[ACGT]AG
DraIII        822  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          323  CCGTGG               C^C[GA][CT]GG
EcoO65I       180  GGTGACC              G^GT[ACGT]ACC
EcoT14I       494  CCAAGG               C^C[AT][AT]GG
Hin1I         913  GGCGTC               G[GA]^CG[CT]C
MaeI          949  CTAG                 C^TAG
PleI          282  GAGTC                GAGTC(4/5)
PshAI         348  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          859  CTGCAG               CTGCA^G
SmlI          246  CTTGAG               C^T[CT][GA]AG
StyI          494  CCAAGG               C^C[AT][AT]GG
TfiI          464  GAATC                G^A[AT]TC
XcmI          900  CCAGCAGACCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII         382  CGCG                 CG^CG
              881  CGCG                
Alw44I         42  GTGCAC               G^TGCAC
              854  GTGCAC              
AlwI          417  GATCC                GGATC(4/5)
               33  GGATC               
ApaLI          42  GTGCAC               G^TGCAC
              854  GTGCAC              
AspHI          42  GTGCAC               G[AT]GC[AT]^C
              854  GTGCAC              
AvaI          277  CCCGAG               C^[CT]CG[GA]G
              556  CCCGAG              
BcgI          846  CGAGAGCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1005  CGATGAGGATGC        
BsgI          150  CTGCAC               GTGCAG(16/14)
              828  GTGCAG              
BsiHKAI        42  GTGCAC               G[AT]GC[AT]^C
              854  GTGCAC              
BsoBI         277  CCCGAG               C^[CT]CG[GA]G
              556  CCCGAG              
BsrI          622  ACTGG                ACTGG(1/-1)
              814  CCAGT               
BstUI         382  CGCG                 CG^CG
              881  CGCG                
BstYI         416  AGATCC               [GA]^GATC[CT]
              452  AGATCT              
Cac8I         103  GCCTGC               GC[ACGT]^[ACGT]GC
              830  GCAGGC              
DraII         539  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              540  GGGCCCC             
EcoO109I      539  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              540  GGGCCCC             
FauI          879  CCCGC                CCCGC(4/6)
              177  GCGGG               
HaeII          95  AGCGCC               [GA]GCGC^[CT]
              552  AGCGCC              
HaeIII        102  GGCC                 GG^CC
              541  GGCC                
HgaI           86  GACGC                GACGC(5/10)
              914  GCGTC               
HinfI         282  GAGTC                G^A[ACGT]TC
              464  GAATC               
MflI          416  AGATCC               [GA]^GATC[CT]
              452  AGATCT              
MslI          562  CATCTACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              776  CATTCAAGTG          
MspA1I        350  CAGCTG               C[AC]G^C[GT]G
              645  CAGCTG              
MvnI          382  CGCG                 CG^CG
              881  CGCG                
PvuII         350  CAGCTG               CAG^CTG
              645  CAGCTG              
SfcI          859  CTGCAG               C^T[GA][CT]AG
              965  CTACAG              
XhoII         416  AGATCC               [GA]^GATC[CT]
              452  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AluI        BanII       BbsI        BbvI        
BcnI        BmyI        BpuAI       BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       BstF5I      
BstNI       CfoI        Cfr13I      DpnII       EarI        
EcoRII      Fnu4HI      FokI        HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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