Restriction map for pF1KB9652
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BfaI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsiWI       
BspDI       BspEI       BspMI       BsrDI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstF5I      
BstXI       BstYI       BstZ17I     Bsu36I      ClaI        
DraI        DraIII      DrdI        Eam1105I    Eco81I      
EcoNI       EcoRI       EcoRV       EcoT22I     FokI        
FseI        HincII      HindII      HindIII     HinfI       
HpaI        HphI        KpnI        MaeI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tsp45I      Tsp509I     
XbaI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AflIII        878  ACATGT               A^C[GA][CT]GT
Aor51HI       294  AGCGCT               AGC^GCT
ApaI           36  GGGCCC               GGGCC^C
Asp700I       135  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         310  GAGCAC               G[AT]GC[AT]^C
AspI          115  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          549  CCCGGG               C^[CT]CG[GA]G
AviII         561  TGCGCA               TGC^GCA
BanII          36  GGGCCC               G[GA]GC[CT]^C
BclI          818  TGATCA               T^GATCA
BglI          381  GCCCGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          280  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      280  GCTAAGC              GC^T[ACGT]AGC
BsgI          298  CTGCAC               GTGCAG(16/14)
BsiHKAI       310  GAGCAC               G[AT]GC[AT]^C
BsmI          135  GAATGC               GAATGC(1/-1)
BsoBI         549  CCCGGG               C^[CT]CG[GA]G
Bsp1407I        2  TGTACA               T^GTACA
BspHI         356  TCATGA               T^CATGA
BspLU11I      878  ACATGT               A^CATGT
BsrGI           2  TGTACA               T^GTACA
BstAPI        582  GCAGCCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        762  GGTTACC              G^GT[ACGT]ACC
BstPI         762  GGTTACC              G^GT[ACGT]ACC
CelII         280  GCTAAGC              GC^T[ACGT]AGC
CpoI          113  CGGACCG              CG^G[AT]CCG
DdeI          281  CTAAG                C^T[ACGT]AG
DraII         803  GGGACCT              [GA]G^G[ACGT]CC[CT]
EarI          768  CTCTTC               CTCTTC(1/4)
Eco47III      294  AGCGCT               AGC^GCT
EcoO109I      803  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       762  GGTTACC              G^GT[ACGT]ACC
EcoT14I       716  CCATGG               C^C[AT][AT]GG
FbaI          818  TGATCA               T^GATCA
FspI          561  TGCGCA               TGC^GCA
Ksp632I       768  CTCTTC               CTCTTC(1/4)
MaeII         602  ACGT                 A^CGT
MseI          923  TTAA                 T^TAA
NcoI          716  CCATGG               C^CATGG
PflFI         115  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         462  CCAGCGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         803  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          613  CTGCAG               CTGCA^G
PvuII         529  CAGCTG               CAG^CTG
RcaI          356  TCATGA               T^CATGA
RsrII         113  CGGACCG              CG^G[AT]CCG
SmaI          549  CCCGGG               CCC^GGG
SspBI           2  TGTACA               T^GTACA
StyI          716  CCATGG               C^C[AT][AT]GG
TaqI          272  TCGA                 T^CGA
Tru9I         923  TTAA                 T^TAA
TspRI         932  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       115  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       272  TCGA                 T^CGA
Van91I        462  CCAGCGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         549  CCCGGG               C^CCGGG
XmaI          549  CCCGGG               C^CCGGG
XmnI          135  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BsiEI          86  CGGCCG               CG[GA][CT]^CG
              262  CGGCCG              
BsmAI          16  GAGAC                GTCTC(1/5)
              250  GAGAC               
BsmBI          16  GAGACG               CGTCTC(1/5)
              250  GAGACG              
BsrBI         420  GAGCGG               CCGCTC(-3/-3)
              897  GAGCGG              
DpnII         210  GATC                 ^GATC
              819  GATC                
EagI           86  CGGCCG               C^GGCCG
              262  CGGCCG              
EclXI          86  CGGCCG               C^GGCCG
              262  CGGCCG              
Eco52I         86  CGGCCG               C^GGCCG
              262  CGGCCG              
HgaI          351  GACGC                GACGC(5/10)
              119  GCGTC               
KspI           77  CCGCGG               CCGC^GG
              156  CCGCGG              
MaeIII        476  GTTAC                ^GT[ACGT]AC
              763  GTTAC               
MboI          210  GATC                 ^GATC
              819  GATC                
MboII         360  GAAGA                GAAGA(8/7)
              769  TCTTC               
NaeI           88  GCCGGC               GCC^GGC
              439  GCCGGC              
NdeII         210  GATC                 ^GATC
              819  GATC                
NgoMIV         88  GCCGGC               G^CCGGC
              439  GCCGGC              
SacII          77  CCGCGG               CCGC^GG
              156  CCGCGG              
Sau3AI        210  GATC                 ^GATC
              819  GATC                
XcmI          716  CCATGGGTTCGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              749  CCAGCCCCCCTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaII       BanI        BbeI        BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrFI       BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DsaI        EaeI        EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HpaII       ItaI        KasI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NlaIII      NlaIV       
NspI        RsaI        Sau96I      ScrFI       SfaNI       
SfcI        SfoI        TseI        
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