Restriction map for pF1KB9652
 (Linear) (Six-base) MAPSORT of: KIBB9652.seq  Check: 327  from: 1  to: 951



KIBB9652.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     951 
   Size:         46     905
 
AfeI AGC'GCT

Cuts at:      0     296     951 
   Size:        296     655
 
AflIII A'CryG_T

Cuts at:      0     878     951 
   Size:        878      73
 
ApaI G_GGCC'C

Cuts at:      0      40     951 
   Size:         40     911
 
AvaI C'yCGr_G

Cuts at:      0     549     951 
   Size:        549     402
 
BanI G'GyrC_C

Cuts at:      0     226     436     691     786     838     846     906     951 
   Size:        226     210     255      95      52       8      60      45

  Fragments arranged by size: 

                 255     226     210      95      60      52      45       8
            
 
BanII G_rGCy'C

Cuts at:      0      40     951 
   Size:         40     911
 
BbeI G_GCGC'C

Cuts at:      0     230     440     790     842     951 
   Size:        230     210     350      52     109

  Fragments arranged by size: 

                 350     230     210     109      52
 
BclI T'GATC_A

Cuts at:      0     818     951 
   Size:        818     133
 
BglI GCCn_nnn'nGGC

Cuts at:      0     387     951 
   Size:        387     564
 
BlpI GC'TnA_GC

Cuts at:      0     281     951 
   Size:        281     670
 
Bme1580I G_kGCm'C

Cuts at:      0      40     694     911     951 
   Size:         40     654     217      40

  Fragments arranged by size: 

                 654     217      40      40
 
BmgBI CAC'GTC

Cuts at:      0     603     951 
   Size:        603     348
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     762     951 
   Size:        762     189
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     516     732     765     951 
   Size:        516     216      33     186

  Fragments arranged by size: 

                 516     216     186      33
 
BsaHI Gr'CG_yC

Cuts at:      0     227     437     787     839     951 
   Size:        227     210     350      52     112

  Fragments arranged by size: 

                 350     227     210     112      52
 
BseYI C'CCAG_C

Cuts at:      0     531     865     884     951 
   Size:        531     334      19      67

  Fragments arranged by size: 

                 531     334      67      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     951 
   Size:        283     668
 
BsiEI CG_ry'CG

Cuts at:      0      89     265     951 
   Size:         89     176     686

  Fragments arranged by size: 

                 686     176      89
 
BsiHKAI G_wGCw'C

Cuts at:      0     314     951 
   Size:        314     637
 
BsmI GAATG_Cn'

Cuts at:      0     141     951 
   Size:        141     810
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      10     244     951 
   Size:         10     234     707

  Fragments arranged by size: 

                 707     234      10
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     314     694     911     951 
   Size:         40     274     380     217      40

  Fragments arranged by size: 

                 380     274     217      40      40
 
BspHI T'CATG_A

Cuts at:      0     356     951 
   Size:        356     595
 
BsrBI CCG'CTC

Cuts at:      0     422     899     951 
   Size:        422     477      52

  Fragments arranged by size: 

                 477     422      52
 
BsrFI r'CCGG_y

Cuts at:      0      88     329     439     951 
   Size:         88     241     110     512

  Fragments arranged by size: 

                 512     241     110      88
 
BsrGI T'GTAC_A

Cuts at:      0       2     951 
   Size:          2     949
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     588     951 
   Size:        588     363
 
BstEII G'GTnAC_C

Cuts at:      0     762     951 
   Size:        762     189
 
BtgI C'CryG_G

Cuts at:      0      77     156     716     951 
   Size:         77      79     560     235

  Fragments arranged by size: 

                 560     235      79      77
 
BtsI GCAGTG_nn'

Cuts at:      0     931     951 
   Size:        931      20
 
EaeI y'GGCC_r

Cuts at:      0      86     262     917     951 
   Size:         86     176     655      34

  Fragments arranged by size: 

                 655     176      86      34
 
EagI C'GGCC_G

Cuts at:      0      86     262     951 
   Size:         86     176     689

  Fragments arranged by size: 

                 689     176      86
 
EarI CTCTTCn'nnn_

Cuts at:      0     774     951 
   Size:        774     177
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     354     951 
   Size:        354     597
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     516     732     765     951 
   Size:         46     470     216      33     186

  Fragments arranged by size: 

                 470     216     186      46      33
 
EcoO109I rG'GnC_Cy

Cuts at:      0     804     951 
   Size:        804     147
 
FspI TGC'GCA

Cuts at:      0     563     951 
   Size:        563     388
 
FspAI rTGC'GCAy

Cuts at:      0     563     951 
   Size:        563     388
 
HaeII r_GCGC'y

Cuts at:      0     222     230     298     440     611     790     842     951 
   Size:        222       8      68     142     171     179      52     109

  Fragments arranged by size: 

                 222     179     171     142     109      68      52       8
            
 
KasI G'GCGC_C

Cuts at:      0     226     436     786     838     951 
   Size:        226     210     350      52     113

  Fragments arranged by size: 

                 350     226     210     113      52
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     951 
   Size:        227     724
 
MspA1I CmG'CkG

Cuts at:      0      79     158     165     531     951 
   Size:         79      79       7     366     420

  Fragments arranged by size: 

                 420     366      79      79       7
 
NaeI GCC'GGC

Cuts at:      0      90     441     951 
   Size:         90     351     510

  Fragments arranged by size: 

                 510     351      90
 
NarI GG'CG_CC

Cuts at:      0     227     437     787     839     951 
   Size:        227     210     350      52     112

  Fragments arranged by size: 

                 350     227     210     112      52
 
NcoI C'CATG_G

Cuts at:      0     716     951 
   Size:        716     235
 
NgoMIV G'CCGG_C

Cuts at:      0      88     439     951 
   Size:         88     351     512

  Fragments arranged by size: 

                 512     351      88
 
NspI r_CATG'y

Cuts at:      0     675     828     882     951 
   Size:        675     153      54      69

  Fragments arranged by size: 

                 675     153      69      54
 
PciI A'CATG_T

Cuts at:      0     878     951 
   Size:        878      73
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     468     951 
   Size:        468     483
 
PfoI T'CCnGG_A

Cuts at:      0     809     951 
   Size:        809     142
 
PpuMI rG'GwC_Cy

Cuts at:      0     804     951 
   Size:        804     147
 
PsiI TTA'TAA

Cuts at:      0     323     951 
   Size:        323     628
 
PspOMI G'GGCC_C

Cuts at:      0      36     951 
   Size:         36     915
 
PstI C_TGCA'G

Cuts at:      0     617     951 
   Size:        617     334
 
PvuII CAG'CTG

Cuts at:      0     531     951 
   Size:        531     420
 
RsrII CG'GwC_CG

Cuts at:      0     114     951 
   Size:        114     837
 
SacII CC_GC'GG

Cuts at:      0      80     159     951 
   Size:         80      79     792

  Fragments arranged by size: 

                 792      80      79
 
SfcI C'TryA_G

Cuts at:      0     510     613     666     951 
   Size:        510     103      53     285

  Fragments arranged by size: 

                 510     285     103      53
 
SfoI GGC'GCC

Cuts at:      0     228     438     788     840     951 
   Size:        228     210     350      52     111

  Fragments arranged by size: 

                 350     228     210     111      52
 
SmaI CCC'GGG

Cuts at:      0     551     951 
   Size:        551     400
 
StyI C'CwwG_G

Cuts at:      0     716     951 
   Size:        716     235
 
TatI w'GTAC_w

Cuts at:      0       2     371     617     951 
   Size:          2     369     246     334

  Fragments arranged by size: 

                 369     334     246       2
 
Tth111I GACn'n_nGTC

Cuts at:      0     118     951 
   Size:        118     833
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     723     756     951 
   Size:        723      33     195

  Fragments arranged by size: 

                 723     195      33
 
XmaI C'CCGG_G

Cuts at:      0     549     951 
   Size:        549     402
 
XmnI GAAnn'nnTTC

Cuts at:      0     139     951 
   Size:        139     812
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     ApaI     AvaI     BanI    BanII     BbeI
     BclI     BglI     BlpI Bme1580I    BmgBI     BplI     BpmI    BsaHI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BsrBI    BsrFI    BsrGI   BstAPI   BstEII     BtgI     BtsI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I     FspI    FspAI    HaeII
     KasI     MmeI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PfoI    PpuMI     PsiI   PspOMI     PstI    PvuII
    RsrII    SacII     SfcI     SfoI     SmaI     StyI     TatI  Tth111I
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
    BfrBI    BglII     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BsiWI    BspEI    BspMI    BsrDI
   BssHII    BssSI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     MslI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI    PspXI
     PsrI     PvuI     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI     XbaI     XhoI
     ZraI
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