Restriction map for pF1KB9625
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AvrII       BamHI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HgaI        
HincII      HindII      HindIII     HpaI        HphI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MboI        MboII       MfeI        MflI        MluI        
MroI        MseI        MspA1I      MunI        NdeI        
NdeII       NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfuI        SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tsp509I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         394  GACGTC               GACGT^C
AfaI          225  GTAC                 GT^AC
ApaI           90  GGGCCC               GGGCC^C
AspI           54  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          134  CCCGAG               C^[CT]CG[GA]G
AviII         293  TGCGCA               TGC^GCA
BalI          356  TGGCCA               TGG^CCA
BanII          90  GGGCCC               G[GA]GC[CT]^C
BbrPI         385  CACGTG               CAC^GTG
BmyI           90  GGGCCC               G[AGT]GC[ACT]^C
BsaAI         385  CACGTG               [CT]AC^GT[GA]
BseRI         190  GAGGAG               GAGGAG(10/8)
BsmAI          52  GAGAC                GTCTC(1/5)
BsoBI         134  CCCGAG               C^[CT]CG[GA]G
Bsp1286I       90  GGGCCC               G[AGT]GC[ACT]^C
BstF5I        255  CATCC                GGATG(2/0)
BstXI          20  CCATGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII          90  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI           20  CCATGG               C^C[GA][CT]GG
EcoO109I       90  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I        20  CCATGG               C^C[AT][AT]GG
FokI          255  CATCC                GGATG(9/13)
FspI          293  TGCGCA               TGC^GCA
MaeIII         71  GTGAC                ^GT[ACGT]AC
MluNI         356  TGGCCA               TGG^CCA
MscI          356  TGGCCA               TGG^CCA
MslI          255  CATCCGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI           20  CCATGG               C^CATGG
NlaIII         21  CATG                 CATG^
PflFI          54  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         384  CCACGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           58  GAGTC                GAGTC(4/5)
PmaCI         385  CACGTG               CAC^GTG
PmlI          385  CACGTG               CAC^GTG
PstI          172  CTGCAG               CTGCA^G
RsaI          225  GTAC                 GT^AC
SfcI          172  CTGCAG               C^T[GA][CT]AG
StyI           20  CCATGG               C^C[AT][AT]GG
TfiI          345  GATTC                G^A[AT]TC
Tsp45I         71  GTGAC                ^GT[GC]AC
TspRI          69  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I        54  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        384  CCACGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AvaII          30  GGACC                G^G[AT]CC
              115  GGTCC               
BbeI          200  GGCGCC               GGCGC^C
              206  GGCGCC              
BspMI          32  ACCTGC               ACCTGC(4/8)
              170  ACCTGC              
BsrFI         197  GCCGGC               [GA]^CCGG[CT]
              203  GCCGGC              
Cfr10I        197  GCCGGC               [GA]^CCGG[CT]
              203  GCCGGC              
EaeI          159  TGGCCG               [CT]^GGCC[GA]
              356  TGGCCA              
FauI           83  GCGGG                CCCGC(4/6)
               88  GCGGG               
HinfI          58  GAGTC                G^A[ACGT]TC
              345  GATTC               
KasI          200  GGCGCC               G^GCGCC
              206  GGCGCC              
MaeII         386  ACGT                 A^CGT
              395  ACGT                
NaeI          197  GCCGGC               GCC^GGC
              203  GCCGGC              
NarI          200  GGCGCC               GG^CGCC
              206  GGCGCC              
NgoMIV        197  GCCGGC               G^CCGGC
              203  GCCGGC              
SfoI          200  GGCGCC               GGC^GCC
              206  GGCGCC              
SgrAI         196  CGCCGGCG             C[GA]^CCGG[CT]G
              202  CGCCGGCG            


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwNI       
BanI        BbvI        BcnI        BsaHI       BsaJI       
BsiYI       BslI        BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        EcoRII      
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HpaII       ItaI        MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NlaIV       Sau96I      ScrFI       SfaNI       TseI        
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