Restriction map for pF1KB9625
 (Linear) (Six-base) MAPSORT of: KIBB9625.seq  Check: 2986  from: 1  to: 399



KIBB9625.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AatII G_ACGT'C

Cuts at:      0     398     399 
   Size:        398       1
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     399 
   Size:         51     348
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     319     399 
   Size:        319      80
 
AlwNI CAG_nnn'CTG

Cuts at:      0      55      74     180     399 
   Size:         55      19     106     219

  Fragments arranged by size: 

                 219     106      55      19
 
ApaI G_GGCC'C

Cuts at:      0      94     399 
   Size:         94     305
 
AvaI C'yCGr_G

Cuts at:      0     134     399 
   Size:        134     265
 
BanI G'GyrC_C

Cuts at:      0     100     200     206     399 
   Size:        100     100       6     193

  Fragments arranged by size: 

                 193     100     100       6
 
BanII G_rGCy'C

Cuts at:      0      94     399 
   Size:         94     305
 
BbeI G_GCGC'C

Cuts at:      0     204     210     399 
   Size:        204       6     189

  Fragments arranged by size: 

                 204     189       6
 
Bme1580I G_kGCm'C

Cuts at:      0      94     399 
   Size:         94     305
 
BsaAI yAC'GTr

Cuts at:      0     387     399 
   Size:        387      12
 
BsaHI Gr'CG_yC

Cuts at:      0     201     207     395     399 
   Size:        201       6     188       4

  Fragments arranged by size: 

                 201     188       6       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205     399 
   Size:        205     194
 
Bsp1286I G_dGCh'C

Cuts at:      0      94     399 
   Size:         94     305
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      41     179     399 
   Size:         41     138     220

  Fragments arranged by size: 

                 220     138      41
 
BsrFI r'CCGG_y

Cuts at:      0     197     203     399 
   Size:        197       6     196

  Fragments arranged by size: 

                 197     196       6
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      27     399 
   Size:         27     372
 
BtgI C'CryG_G

Cuts at:      0      20     399 
   Size:         20     379
 
EaeI y'GGCC_r

Cuts at:      0     159     356     399 
   Size:        159     197      43

  Fragments arranged by size: 

                 197     159      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     399 
   Size:         51     348
 
EcoO109I rG'GnC_Cy

Cuts at:      0      91     399 
   Size:         91     308
 
FspI TGC'GCA

Cuts at:      0     295     399 
   Size:        295     104
 
HaeII r_GCGC'y

Cuts at:      0     198     204     210     399 
   Size:        198       6       6     189

  Fragments arranged by size: 

                 198     189       6       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      43      75     239     271     399 
   Size:         43      32     164      32     128

  Fragments arranged by size: 

                 164     128      43      32      32
 
KasI G'GCGC_C

Cuts at:      0     200     206     399 
   Size:        200       6     193

  Fragments arranged by size: 

                 200     193       6
 
MscI TGG'CCA

Cuts at:      0     358     399 
   Size:        358      41
 
MslI CAynn'nnrTG

Cuts at:      0     259     399 
   Size:        259     140
 
NaeI GCC'GGC

Cuts at:      0     199     205     399 
   Size:        199       6     194

  Fragments arranged by size: 

                 199     194       6
 
NarI GG'CG_CC

Cuts at:      0     201     207     399 
   Size:        201       6     192

  Fragments arranged by size: 

                 201     192       6
 
NcoI C'CATG_G

Cuts at:      0      20     399 
   Size:         20     379
 
NgoMIV G'CCGG_C

Cuts at:      0     197     203     399 
   Size:        197       6     196

  Fragments arranged by size: 

                 197     196       6
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     390     399 
   Size:        390       9
 
PfoI T'CCnGG_A

Cuts at:      0     117     399 
   Size:        117     282
 
PmlI CAC'GTG

Cuts at:      0     387     399 
   Size:        387      12
 
PspOMI G'GGCC_C

Cuts at:      0      90     399 
   Size:         90     309
 
PstI C_TGCA'G

Cuts at:      0     176     399 
   Size:        176     223
 
SfcI C'TryA_G

Cuts at:      0     172     399 
   Size:        172     227
 
SfoI GGC'GCC

Cuts at:      0     202     208     399 
   Size:        202       6     191

  Fragments arranged by size: 

                 202     191       6
 
SgrAI Cr'CCGG_yG

Cuts at:      0     197     203     399 
   Size:        197       6     196

  Fragments arranged by size: 

                 197     196       6
 
StyI C'CwwG_G

Cuts at:      0      20     399 
   Size:         20     379
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      58     399 
   Size:         58     341
 
Tth111I GACn'n_nGTC

Cuts at:      0      57     399 
   Size:         57     342
 
ZraI GAC'GTC

Cuts at:      0     396     399 
   Size:        396       3
 

 Enzymes that do cut:

    AatII     AcuI     AlfI    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI Bme1580I    BsaAI    BsaHI    BseRI Bsp1286I    BspMI    BsrFI
    BstXI     BtgI     EaeI  Eco57MI EcoO109I     FspI    HaeII    Hin4I
     KasI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV    PflMI
     PfoI     PmlI   PspOMI     PstI     SfcI     SfoI    SgrAI     StyI
    TaqII  Tth111I     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI   MspA1I     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI     XbaI     XcmI     XhoI     XmaI
     XmnI
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