Restriction map for pF1KB9624
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        Asp700I     
Asp718I     AspHI       AvaI        AviII       AvrII       
BamHI       BbeI        BbsI        BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmI        BsoBI       BspEI       
BspHI       BspLU11I    BsrDI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       CfoI        CpoI        
DraI        DraIII      EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        HinP1I      HindIII     HpaI        
KasI        KpnI        MaeI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tsp509I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII         292  GACGTC               GACGT^C
AcyI          292  GACGTC               G[GA]^CG[CT]C
AflIII       1005  ACACGT               A^C[GA][CT]GT
AhdI          597  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI           97  GGGCCC               GGGCC^C
AseI          889  ATTAAT               AT^TAAT
AsnI          889  ATTAAT               AT^TAAT
AspEI         597  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          463  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          764  TGGCCA               TGG^CCA
BbrPI        1006  CACGTG               CAC^GTG
BbvCI         528  CCTCAGC              CCTCAGC(-5/-2)
BglI          962  GCCCAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         176  AGATCT               A^GATCT
BsaAI        1006  CACGTG               [CT]AC^GT[GA]
BsaBI         453  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         292  GACGTC               G[GA]^CG[CT]C
BsaI          563  GAGACC               GGTCTC(1/5)
BsmBI         900  GAGACG               CGTCTC(1/5)
Bsp1407I      585  TGTACA               T^GTACA
BspDI         922  ATCGAT               AT^CGAT
BsrBI         190  CCGCTC               CCGCTC(-3/-3)
BsrFI         113  ACCGGC               [GA]^CCGG[CT]
BsrGI         585  TGTACA               T^GTACA
BstXI         458  CCATCGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        113  ACCGGC               [GA]^CCGG[CT]
ClaI          922  ATCGAT               AT^CGAT
DrdI          652  GACCAAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          764  TGGCCA               [CT]^GGCC[GA]
Eam1105I      597  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI          902  GACGC                GACGC(5/10)
Hin1I         292  GACGTC               G[GA]^CG[CT]C
HincII        874  GTCAAC               GT[CT]^[GA]AC
HindII        874  GTCAAC               GT[CT]^[GA]AC
MaeIII        650  GTGAC                ^GT[ACGT]AC
MamI          453  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         764  TGGCCA               TGG^CCA
MscI          764  TGGCCA               TGG^CCA
MseI          890  TTAA                 T^TAA
NspI          238  ACATGC               [GA]CATG^[CT]
PflFI         463  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         426  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          659  GAGTC                GAGTC(4/5)
PmaCI        1006  CACGTG               CAC^GTG
PmlI         1006  CACGTG               CAC^GTG
PpuMI         595  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         889  ATTAAT               AT^TAAT
PstI          492  CTGCAG               CTGCA^G
PvuII         838  CAGCTG               CAG^CTG
SapI          192  GCTCTTC              GCTCTTC(1/4)
SexAI         464  ACCTGGT              A^CC[AT]GGT
SfcI          492  CTGCAG               C^T[GA][CT]AG
SspBI         585  TGTACA               T^GTACA
Tru9I         890  TTAA                 T^TAA
Tsp45I        650  GTGAC                ^GT[GC]AC
Tth111I       463  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        426  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BanI           90  GGCACC               G^G[CT][GA]CC
              806  GGCACC              
BanII          97  GGGCCC               G[GA]GC[CT]^C
              856  GGGCTC              
BcnI            5  CCCGG                CC^[GC]GG
              896  CCCGG               
BsgI          844  GTGCAG               GTGCAG(16/14)
              950  GTGCAG              
BspMI         428  ACCTGC               ACCTGC(4/8)
              342  GCAGGT              
BstYI         176  AGATCT               [GA]^GATC[CT]
              749  GGATCT              
DdeI          353  CTGAG                C^T[ACGT]AG
              529  CTCAG               
EcoT14I       654  CCAAGG               C^C[AT][AT]GG
              758  CCAAGG              
HphI          111  TCACC                GGTGA(8/7)
              860  TCACC               
KspI           86  CCGCGG               CCGC^GG
              282  CCGCGG              
MaeII         293  ACGT                 A^CGT
             1007  ACGT                
MflI          176  AGATCT               [GA]^GATC[CT]
              749  GGATCT              
MslI          408  CATCCAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              672  CACCAGCGTG          
NciI            5  CCCGG                CC^[GC]GG
              896  CCCGG               
SacII          86  CCGCGG               CCGC^GG
              282  CCGCGG              
StyI          654  CCAAGG               C^C[AT][AT]GG
              758  CCAAGG              
TfiI          307  GATTC                G^A[AT]TC
              558  GATTC               
XcmI          374  CCAACCCAGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              611  CCAGCGGGGGCCTGG     
XhoII         176  AGATCT               [GA]^GATC[CT]
              749  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BbvI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       Cfr13I      DpnII       DraII       
DsaI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        Ksp632I     MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TseI        TspRI       TthHB8I     
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