Restriction map for pF1KB9623
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# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AvaII       AviII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmAI       BsmBI       BsmFI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tsp509I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AclI          165  AACGTT               AA^CGTT
AfaI          130  GTAC                 GT^AC
Asp700I       237  GAACCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         180  GAGCTC               G[AT]GC[AT]^C
AspI          210  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          193  CCCGAG               C^[CT]CG[GA]G
AvrII         342  CCTAGG               C^CTAGG
BanII         180  GAGCTC               G[GA]GC[CT]^C
BbsI          403  GAAGAC               GAAGAC(2/6)
BfaI          343  CTAG                 C^TAG
BlnI          342  CCTAGG               C^CTAGG
BpuAI         403  GAAGAC               GAAGAC(2/6)
BsiHKAI       180  GAGCTC               G[AT]GC[AT]^C
BsmI          268  GCATTC               GAATGC(1/-1)
BsoBI         193  CCCGAG               C^[CT]CG[GA]G
BssSI         226  CTCGTG               CACGAG(-5/-1)
BstNI         126  CCAGG                CC^[AT]GG
BstXI         257  CCATGAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI           55  CTGAG                C^T[ACGT]AG
EarI          421  GAAGAG               CTCTTC(1/4)
EcoRII        126  CCAGG                ^CC[AT]GG
EcoT14I       342  CCTAGG               C^C[AT][AT]GG
HinfI         242  GATTC                G^A[ACGT]TC
Ksp632I       421  GAAGAG               CTCTTC(1/4)
MaeI          343  CTAG                 C^TAG
MseI          170  TTAA                 T^TAA
MspA1I         85  CAGCTG               C[AC]G^C[GT]G
MvaI          126  CCAGG                CC^[AT]GG
NspI          221  ACATGC               [GA]CATG^[CT]
PflFI         210  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          72  CCAGCTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I      165  AACGTT               AA^CGTT
PvuII          85  CAGCTG               CAG^CTG
RsaI          130  GTAC                 GT^AC
SacI          180  GAGCTC               GAGCT^C
StyI          342  CCTAGG               C^C[AT][AT]GG
TfiI          242  GATTC                G^A[AT]TC
Tru9I         170  TTAA                 T^TAA
TspRI         379  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       210  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         72  CCAGCTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          237  GAACCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccII          30  CGCG                 CG^CG
               49  CGCG                
AcyI          213  GGCGTC               G[GA]^CG[CT]C
              253  GACGCC              
BsaHI         213  GGCGTC               G[GA]^CG[CT]C
              253  GACGCC              
BsgI           25  CTGCAC               GTGCAG(16/14)
              205  GTGCAG              
BstUI          30  CGCG                 CG^CG
               49  CGCG                
Cfr13I        110  GGGCC                G^G[ACGT]CC
              356  GGGCC               
HaeIII        111  GGCC                 GG^CC
              357  GGCC                
HgaI          253  GACGC                GACGC(5/10)
              214  GCGTC               
Hin1I         213  GGCGTC               G[GA]^CG[CT]C
              253  GACGCC              
HphI          346  GGTGA                GGTGA(8/7)
              287  TCACC               
MaeII         132  ACGT                 A^CGT
              166  ACGT                
MvnI           30  CGCG                 CG^CG
               49  CGCG                
Sau96I        110  GGGCC                G^G[ACGT]CC
              356  GGGCC               
SfaNI          39  GATGC                GCATC(5/9)
               59  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        BanI        BbvI        BcnI        
BmyI        BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      Cac8I       CfoI        
Fnu4HI      FokI        HapII       HhaI        HinP1I      
HpaII       ItaI        MboII       MnlI        MslI        
MspI        MwoI        NciI        NlaIII      NlaIV       
ScrFI       TaqI        TseI        TthHB8I     
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