Restriction map for
pF1KB9616
(Circular) (Six-base) MAPSORT of: pF1KB9616.seq Check: 6589 from: 1 to: 3782
pF1KB9616 3782 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 820 820
Size: 3782
Acc65I G'GTAC_C
Cuts at: 798 798
Size: 3782
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
AflIII A'CryG_T
Cuts at: 1134 2238 3215 1134
Size: 1104 977 1701
Fragments arranged by size:
1701 1104 977
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 1087 2654 1087
Size: 1567 2215
ApaLI G'TGCA_C
Cuts at: 2552 2552
Size: 3782
ApoI r'AATT_y
Cuts at: 786 3135 3391 786
Size: 2349 256 1177
Fragments arranged by size:
2349 1177 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3782
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 236 271 277 329 516 802 236
Size: 35 6 52 187 286 3216
Fragments arranged by size:
3216 286 187 52 35 6
BamHI G'GATC_C
Cuts at: 807 807
Size: 3782
BanI G'GyrC_C
Cuts at: 408 613 693 798 1212 1460 1495 408
Size: 205 80 105 414 248 35 2695
Fragments arranged by size:
2695 414 248 205 105 80 35
BanII G_rGCy'C
Cuts at: 245 278 796 1826 245
Size: 33 518 1030 2201
Fragments arranged by size:
2201 1030 518 33
BbeI G_GCGC'C
Cuts at: 412 617 697 1216 1464 412
Size: 205 80 519 248 2730
Fragments arranged by size:
2730 519 248 205 80
BciVI GTATCCnnnnn_n'
Cuts at: 1680 2441 3782 1680
Size: 761 1341 1680
Fragments arranged by size:
1680 1341 761
BclI T'GATC_A
Cuts at: 1302 1302
Size: 3782
BglI GCCn_nnn'nGGC
Cuts at: 769 769
Size: 3782
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 894 894
Size: 3782
Bme1580I G_kGCm'C
Cuts at: 444 1407 1500 2556 444
Size: 963 93 1056 1670
Fragments arranged by size:
1670 1056 963 93
BmrI ACTGGGnnnn_n'
Cuts at: 994 1075 1410 3554 994
Size: 81 335 2144 1222
Fragments arranged by size:
2144 1222 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 444 2149 444
Size: 1705 2077
Bpu10I CC'TnA_GC
Cuts at: 487 487
Size: 3782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1837 2329 2627 958
Size: 879 492 298 2113
Fragments arranged by size:
2113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 418 3486 418
Size: 3068 714
BsaAI yAC'GTr
Cuts at: 1135 1135
Size: 3782
BsaBI GATnn'nnATC
Cuts at: 6 1296 3333 6
Size: 1290 2037 455
Fragments arranged by size:
2037 1290 455
BsaHI Gr'CG_yC
Cuts at: 409 614 694 1213 1461 2163 3659 409
Size: 205 80 519 248 702 1496 532
Fragments arranged by size:
1496 702 532 519 248 205 80
BsaWI w'CCGG_w
Cuts at: 200 384 971 1492 2170 2444 2591 3002 200
Size: 184 587 521 678 274 147 411 980
Fragments arranged by size:
980 678 587 521 411 274 184 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 547
Size: 30 3752
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 568 436
Size: 132 3650
BseYI C'CCAG_C
Cuts at: 1207 1965 2542 3038 1207
Size: 758 577 496 1951
Fragments arranged by size:
1951 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 181 304 136
Size: 45 123 3614
Fragments arranged by size:
3614 123 45
BsiEI CG_ry'CG
Cuts at: 104 296 350 582 1324 1370 2578 104
Size: 192 54 232 742 46 1208 1308
Fragments arranged by size:
1308 1208 742 232 192 54 46
BsiHKAI G_wGCw'C
Cuts at: 245 377 755 796 1574 1764 2556 245
Size: 132 378 41 778 190 792 1471
Fragments arranged by size:
1471 792 778 378 190 132 41
Bsp1286I G_dGCh'C
Cuts at: 245 278 377 444 755 796 1407 1500 1574
Size: 33 99 67 311 41 611 93 74
Cuts at: 1574 1764 1826 2556 245
Size: 190 62 730 1471
Fragments arranged by size:
1471 730 611 311 190 99 93 74
67 62 41 33
BspEI T'CCGG_A
Cuts at: 384 3002 384
Size: 2618 1164
BspHI T'CATG_A
Cuts at: 137 2958 137
Size: 2821 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1046 1348 1729 832
Size: 214 302 381 2885
Fragments arranged by size:
2885 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2074 2128 3612 3777 50
Size: 2024 54 1484 165 55
Fragments arranged by size:
2024 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1694 1694
Size: 3782
BsrFI r'CCGG_y
Cuts at: 291 1780 1961 2170 291
Size: 1489 181 209 1903
Fragments arranged by size:
1903 1489 209 181
BssSI C'ACGA_G
Cuts at: 754 2053 2411 754
Size: 1299 358 2125
Fragments arranged by size:
2125 1299 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1159 1159
Size: 3782
BstBI TT'CG_AA
Cuts at: 2143 2143
Size: 3782
BstYI r'GATC_y
Cuts at: 1 807 1274 1297 1632 1878 2890 2976 2988
Size: 806 467 23 335 246 1012 86 12
Cuts at: 2988 3334 1
Size: 346 449
Fragments arranged by size:
1012 806 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 344 677 764 107
Size: 237 333 87 3125
Fragments arranged by size:
3125 333 237 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1732 1913 2076 1732
Size: 181 163 3438
Fragments arranged by size:
3438 181 163
BtsI GCAGTG_nn'
Cuts at: 1025 1025
Size: 3782
DraI TTT'AAA
Cuts at: 781 781
Size: 3782
DrdI GACnn_nn'nnGTC
Cuts at: 1488 2346 1488
Size: 858 2924
EaeI y'GGCC_r
Cuts at: 254 293 347 1367 1541 1932 1959 3304 254
Size: 39 54 1020 174 391 27 1345 732
Fragments arranged by size:
1345 1020 732 391 174 54 39 27
EagI C'GGCC_G
Cuts at: 293 347 1367 293
Size: 54 1020 2708
Fragments arranged by size:
2708 1020 54
EarI CTCTTCn'nnn_
Cuts at: 545 1805 2015 545
Size: 1260 210 2312
Fragments arranged by size:
2312 1260 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 420 748 1990 2300 2446 3592 420
Size: 328 1242 310 146 1146 610
Fragments arranged by size:
1242 1146 610 328 310 146
EcoICRI GAG'CTC
Cuts at: 243 794 243
Size: 551 3231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 267 921 267
Size: 654 3128
EcoRI G'AATT_C
Cuts at: 786 786
Size: 3782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1801 1801
Size: 3782
FspI TGC'GCA
Cuts at: 229 1563 229
Size: 1334 2448
HaeII r_GCGC'y
Cuts at: 236 412 617 651 697 1216 1464 2486 3465
Size: 176 205 34 46 519 248 1022 979
Cuts at: 3465 236
Size: 553
Fragments arranged by size:
1022 979 553 519 248 205 176 46
34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 1624 1654 1656 1686 547
Size: 32 1045 30 2 30 2643
Fragments arranged by size:
2643 1045 32 30 30 2
HincII GTy'rAC
Cuts at: 821 821
Size: 3782
KasI G'GCGC_C
Cuts at: 408 613 693 1212 1460 408
Size: 205 80 519 248 2730
Fragments arranged by size:
2730 519 248 205 80
KpnI G_GTAC'C
Cuts at: 802 802
Size: 3782
MluI A'CGCG_T
Cuts at: 3215 3215
Size: 3782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 712 2453 2637 712
Size: 1741 184 1857
Fragments arranged by size:
1857 1741 184
MscI TGG'CCA
Cuts at: 1543 1543
Size: 3782
MslI CAynn'nnrTG
Cuts at: 112 1771 1898 112
Size: 1659 127 1996
Fragments arranged by size:
1996 1659 127
MspA1I CmG'CkG
Cuts at: 213 679 893 1207 1567 2580 2825 213
Size: 466 214 314 360 1013 245 1170
Fragments arranged by size:
1170 1013 466 360 314 245 214
NaeI GCC'GGC
Cuts at: 293 1963 293
Size: 1670 2112
NarI GG'CG_CC
Cuts at: 409 614 694 1213 1461 409
Size: 205 80 519 248 2730
Fragments arranged by size:
2730 519 248 205 80
NcoI C'CATG_G
Cuts at: 107 107
Size: 3782
NgoMIV G'CCGG_C
Cuts at: 291 1961 291
Size: 1670 2112
NspI r_CATG'y
Cuts at: 177 744 835 1335 2242 177
Size: 567 91 500 907 1717
Fragments arranged by size:
1717 907 567 500 91
PciI A'CATG_T
Cuts at: 2238 2238
Size: 3782
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PspXI vC'TCGA_Gb
Cuts at: 271 271
Size: 3782
PstI C_TGCA'G
Cuts at: 212 590 644 673 829 1514 212
Size: 378 54 29 156 685 2480
Fragments arranged by size:
2480 685 378 156 54 29
PvuI CG_AT'CG
Cuts at: 104 582 104
Size: 478 3304
PvuII CAG'CTG
Cuts at: 213 1207 1567 213
Size: 994 360 2428
Fragments arranged by size:
2428 994 360
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 245 796 245
Size: 551 3231
SacII CC_GC'GG
Cuts at: 680 680
Size: 3782
SalI G'TCGA_C
Cuts at: 819 819
Size: 3782
SapI GCTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
SbfI CC_TGCA'GG
Cuts at: 829 829
Size: 3782
ScaI AGT'ACT
Cuts at: 3299 3299
Size: 3782
SfcI C'TryA_G
Cuts at: 33 208 586 640 669 825 1510 2503 2694
Size: 175 378 54 29 156 685 993 191
Cuts at: 2694 33
Size: 1121
Fragments arranged by size:
1121 993 685 378 191 175 156 54
29
SfiI GGCCn_nnn'nGGCC
Cuts at: 769 769
Size: 3782
SfoI GGC'GCC
Cuts at: 410 615 695 1214 1462 410
Size: 205 80 519 248 2730
Fragments arranged by size:
2730 519 248 205 80
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 238 804 238
Size: 566 3216
SmlI C'TyrA_G
Cuts at: 271 937 1852 2344 2606 271
Size: 666 915 492 262 1447
Fragments arranged by size:
1447 915 666 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3782
SphI G_CATG'C
Cuts at: 177 744 835 1335 177
Size: 567 91 500 2624
Fragments arranged by size:
2624 567 500 91
StyI C'CwwG_G
Cuts at: 107 257 916 1270 107
Size: 150 659 354 2619
Fragments arranged by size:
2619 659 354 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1365 1498 1957 2166 1365
Size: 133 459 209 2981
Fragments arranged by size:
2981 459 209 133
TatI w'GTAC_w
Cuts at: 3297 3297
Size: 3782
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 3750
Tth111I GACn'n_nGTC
Cuts at: 144 1579 144
Size: 1435 2347
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1277 1277
Size: 3782
XhoI C'TCGA_G
Cuts at: 271 271
Size: 3782
XmaI C'CCGG_G
Cuts at: 236 802 236
Size: 566 3216
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BpmI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PsrI SanDI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI