Restriction map for pF1KB9606
 (Linear) (Six-base) MAPSORT of: KIBB9606.seq  Check: 8879  from: 1  to: 1077



KIBB9606.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

Acc65I G'GTAC_C

Cuts at:      0     818    1077 
   Size:        818     259
 
AhdI GACnn_n'nnGTC

Cuts at:      0     288    1077 
   Size:        288     789
 
AlwNI CAG_nnn'CTG

Cuts at:      0      61    1017    1077 
   Size:         61     956      60

  Fragments arranged by size: 

                 956      61      60
 
AvaI C'yCGr_G

Cuts at:      0     121     337    1077 
   Size:        121     216     740

  Fragments arranged by size: 

                 740     216     121
 
BamHI G'GATC_C

Cuts at:      0     563    1077 
   Size:        563     514
 
BanI G'GyrC_C

Cuts at:      0     117     818    1077 
   Size:        117     701     259

  Fragments arranged by size: 

                 701     259     117
 
BanII G_rGCy'C

Cuts at:      0     396     860    1077 
   Size:        396     464     217

  Fragments arranged by size: 

                 464     396     217
 
BclI T'GATC_A

Cuts at:      0     633    1077 
   Size:        633     444
 
BglI GCCn_nnn'nGGC

Cuts at:      0     101    1077 
   Size:        101     976
 
Bme1580I G_kGCm'C

Cuts at:      0     122    1077 
   Size:        122     955
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     153    1077 
   Size:        153     924
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901     949     994    1000    1077 
   Size:        901      48      45       6      77

  Fragments arranged by size: 

                 901      77      48      45       6
 
Bpu10I CC'TnA_GC

Cuts at:      0      89    1077 
   Size:         89     988
 
BsaHI Gr'CG_yC

Cuts at:      0     334     465    1077 
   Size:        334     131     612

  Fragments arranged by size: 

                 612     334     131
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     139     430     661     754    1077 
   Size:        124      15     291     231      93     323

  Fragments arranged by size: 

                 323     291     231     124      93      15
 
BseYI C'CCAG_C

Cuts at:      0     356     441     942    1077 
   Size:        356      85     501     135

  Fragments arranged by size: 

                 501     356     135      85
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1018    1077 
   Size:       1018      59
 
BsiEI CG_ry'CG

Cuts at:      0     599    1077 
   Size:        599     478
 
BsiHKAI G_wGCw'C

Cuts at:      0     860    1077 
   Size:        860     217
 
BsiWI C'GTAC_G

Cuts at:      0     809    1077 
   Size:        809     268
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     456     799    1077 
   Size:        456     343     278

  Fragments arranged by size: 

                 456     343     278
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     396     860    1077 
   Size:        122     274     464     217

  Fragments arranged by size: 

                 464     274     217     122
 
BspHI T'CATG_A

Cuts at:      0     366    1077 
   Size:        366     711
 
BsrBI CCG'CTC

Cuts at:      0     114     816    1077 
   Size:        114     702     261

  Fragments arranged by size: 

                 702     261     114
 
BsrFI r'CCGG_y

Cuts at:      0     315     594     664     917     989    1037    1077 
   Size:        315     279      70     253      72      48      40

  Fragments arranged by size: 

                 315     279     253      72      70      48      40
 
BsrGI T'GTAC_A

Cuts at:      0      52    1077 
   Size:         52    1025
 
BssHII G'CGCG_C

Cuts at:      0     996    1077 
   Size:        996      81
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     166     387    1077 
   Size:        166     221     690

  Fragments arranged by size: 

                 690     221     166
 
BstYI r'GATC_y

Cuts at:      0     563     654    1077 
   Size:        563      91     423

  Fragments arranged by size: 

                 563     423      91
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     725     770    1077 
   Size:        725      45     307

  Fragments arranged by size: 

                 725     307      45
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     200     235    1077 
   Size:        200      35     842

  Fragments arranged by size: 

                 842     200      35
 
EaeI y'GGCC_r

Cuts at:      0       2     168    1077 
   Size:          2     166     909

  Fragments arranged by size: 

                 909     166       2
 
EarI CTCTTCn'nnn_

Cuts at:      0      71     407     512    1077 
   Size:         71     336     105     565

  Fragments arranged by size: 

                 565     336     105      71
 
EcoICRI GAG'CTC

Cuts at:      0     858    1077 
   Size:        858     219
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901     949     994    1000    1077 
   Size:        901      48      45       6      77

  Fragments arranged by size: 

                 901      77      48      45       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     256     401    1077 
   Size:        256     145     676

  Fragments arranged by size: 

                 676     256     145
 
HaeII r_GCGC'y

Cuts at:      0      96    1057    1077 
   Size:         96     961      20

  Fragments arranged by size: 

                 961      96      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     274     306    1077 
   Size:        274      32     771

  Fragments arranged by size: 

                 771     274      32
 
KpnI G_GTAC'C

Cuts at:      0     822    1077 
   Size:        822     255
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     722    1077 
   Size:        722     355
 
MspA1I CmG'CkG

Cuts at:      0     295     441     792    1014    1077 
   Size:        295     146     351     222      63

  Fragments arranged by size: 

                 351     295     222     146      63
 
NaeI GCC'GGC

Cuts at:      0     317    1077 
   Size:        317     760
 
NgoMIV G'CCGG_C

Cuts at:      0     315    1077 
   Size:        315     762
 
PfoI T'CCnGG_A

Cuts at:      0      71    1077 
   Size:         71    1006
 
PshAI GACnn'nnGTC

Cuts at:      0     468    1077 
   Size:        468     609
 
PstI C_TGCA'G

Cuts at:      0     791     839    1077 
   Size:        791      48     238

  Fragments arranged by size: 

                 791     238      48
 
PvuII CAG'CTG

Cuts at:      0     441    1014    1077 
   Size:        441     573      63

  Fragments arranged by size: 

                 573     441      63
 
SacI G_AGCT'C

Cuts at:      0     860    1077 
   Size:        860     217
 
SapI GCTCTTCn'nnn_

Cuts at:      0      71    1077 
   Size:         71    1006
 
SfcI C'TryA_G

Cuts at:      0     787     835    1077 
   Size:        787      48     242

  Fragments arranged by size: 

                 787     242      48
 
StuI AGG'CCT

Cuts at:      0     232    1077 
   Size:        232     845
 
StyI C'CwwG_G

Cuts at:      0     584    1077 
   Size:        584     493
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     154     934    1036    1077 
   Size:        154     780     102      41

  Fragments arranged by size: 

                 780     154     102      41
 
TatI w'GTAC_w

Cuts at:      0      52     671    1077 
   Size:         52     619     406

  Fragments arranged by size: 

                 619     406      52
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     121     153    1077 
   Size:        121      32     924

  Fragments arranged by size: 

                 924     121      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     529    1077 
   Size:        529     548
 

 Enzymes that do cut:

   Acc65I     AhdI    AlwNI     AvaI    BamHI     BanI    BanII     BclI
     BglI Bme1580I     BmrI     BpmI   Bpu10I    BsaHI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BstXI    BstYI    BtgZI    CspCI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI    HaeII    Hin4I     KpnI     MmeI
   MspA1I     NaeI   NgoMIV     PfoI    PshAI     PstI    PvuII     SacI
     SapI     SfcI     StuI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI     BsmI    BspEI    BspMI
    BsrDI    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MscI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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