################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BpmI BsaAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI Eam1105I EarI EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HinfI HpaI KpnI Ksp632I MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NciI NdeI NdeII NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AfaI 498 GTAC GT^AC AocI 224 CCTCAGG CC^T[ACGT]AGG Aor51HI 269 AGCGCT AGC^GCT AscI 363 GGCGCGCC GG^CGCGCC AspHI 430 GAGCAC G[AT]GC[AT]^C AvaI 673 CCCGAG C^[CT]CG[GA]G BanII 651 GGGCTC G[GA]GC[CT]^C BbsI 276 GTCTTC GAAGAC(2/6) BlpI 666 GCTCAGC GC^T[ACGT]AGC Bpu1102I 666 GCTCAGC GC^T[ACGT]AGC BpuAI 276 GTCTTC GAAGAC(2/6) BseRI 676 GAGGAG GAGGAG(10/8) BsiHKAI 430 GAGCAC G[AT]GC[AT]^C BsmFI 436 GTCCC GGGAC(10/14) BsoBI 673 CCCGAG C^[CT]CG[GA]G BsrI 704 ACTGG ACTGG(1/-1) BstAPI 513 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 224 CCTCAGG CC^T[ACGT]AGG CelII 666 GCTCAGC GC^T[ACGT]AGC Eco47III 269 AGCGCT AGC^GCT Eco81I 224 CCTCAGG CC^T[ACGT]AGG EcoT14I 611 CCATGG C^C[AT][AT]GG HincII 403 GTCAAC GT[CT]^[GA]AC HindII 403 GTCAAC GT[CT]^[GA]AC KspI 110 CCGCGG CCGC^GG MaeII 434 ACGT A^CGT MaeIII 236 GTCAC ^GT[ACGT]AC NcoI 611 CCATGG C^CATGG NotI 115 GCGGCCGC GC^GGCCGC PstI 511 CTGCAG CTGCA^G RsaI 498 GTAC GT^AC SacII 110 CCGCGG CCGC^GG SfaNI 291 GATGC GCATC(5/9) StyI 611 CCATGG C^C[AT][AT]GG Tsp45I 236 GTCAC ^GT[GC]AC TspRI 479 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwNI 514 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 517 CAGCTGCTG BanI 31 GGCGCC G^G[CT][GA]CC 189 GGCGCC BbeI 31 GGCGCC GGCGC^C 189 GGCGCC BmyI 430 GAGCAC G[AGT]GC[ACT]^C 651 GGGCTC BsaI 201 GAGACC GGTCTC(1/5) 627 GGTCTC BsiYI 223 CCCTCAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 419 CCACCCTTCGG BslI 223 CCCTCAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 419 CCACCCTTCGG Bsp1286I 430 GAGCAC G[AGT]GC[ACT]^C 651 GGGCTC BsrBI 664 CCGCTC CCGCTC(-3/-3) 24 GAGCGG BssKI 408 CCTGG ^CC[ACGT]GG 555 CCAGG BstF5I 501 CATCC GGATG(2/0) 632 CATCC BstNI 408 CCTGG CC^[AT]GG 555 CCAGG EcoRII 408 CCTGG ^CC[AT]GG 555 CCAGG FauI 79 CCCGC CCCGC(4/6) 663 CCCGC FokI 501 CATCC GGATG(9/13) 632 CATCC HphI 540 GGTGA GGTGA(8/7) 698 TCACC KasI 31 GGCGCC G^GCGCC 189 GGCGCC MboII 459 GAAGA GAAGA(8/7) 277 TCTTC MvaI 408 CCTGG CC^[AT]GG 555 CCAGG NarI 31 GGCGCC GG^CGCC 189 GGCGCC NlaIII 532 CATG CATG^ 612 CATG NlaIV 31 GGCGCC GG[ACGT]^[ACGT]CC 189 GGCGCC PvuII 196 CAGCTG CAG^CTG 517 CAGCTG ScrFI 408 CCTGG CC^[ACGT]GG 555 CCAGG SfcI 330 CTACAG C^T[GA][CT]AG 511 CTGCAG SfoI 31 GGCGCC GGC^GCC 189 GGCGCC TaqI 494 TCGA T^CGA 692 TCGA TthHB8I 494 TCGA T^CGA 692 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AccII AciI AcyI AluI BbvI BglI BsaHI BsaJI BsiEI BsmAI BsrFI BssHII BstUI Cac8I CfoI Cfr10I DdeI DsaI EaeI EagI EclXI Eco52I Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MnlI MspA1I MspI MvnI MwoI NaeI NgoMIV TseI