(Circular) (Six-base) MAPSORT of: pF1KB9577.seq Check: 8248 from: 1 to: 6746 pF1KB9577 6746 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1843 2605 3312 1843 Size: 762 707 5277 Fragments arranged by size: 5277 762 707 AccI GT'mk_AC Cuts at: 3784 3784 Size: 6746 Acc65I G'GTAC_C Cuts at: 3762 3762 Size: 6746 AclI AA'CG_TT Cuts at: 6586 6586 Size: 6746 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 922 4571 5003 5356 6018 922 Size: 3649 432 353 662 1650 Fragments arranged by size: 3649 1650 662 432 353 AflII C'TTAA_G Cuts at: 1782 1782 Size: 6746 AflIII A'CryG_T Cuts at: 1685 2052 2753 2909 4098 5898 6179 1685 Size: 367 701 156 1189 1800 281 2252 Fragments arranged by size: 2252 1800 1189 701 367 281 156 AgeI A'CCGG_T Cuts at: 3023 3023 Size: 6746 AleI CACnn'nnGTG Cuts at: 628 2066 2752 3103 628 Size: 1438 686 351 4271 Fragments arranged by size: 4271 1438 686 351 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 3010 3042 2042 Size: 32 936 32 5746 Fragments arranged by size: 5746 936 32 32 AlwNI CAG_nnn'CTG Cuts at: 1303 3237 3654 4051 5489 1303 Size: 1934 417 397 1438 2560 Fragments arranged by size: 2560 1934 1438 417 397 ApaLI G'TGCA_C Cuts at: 5584 5584 Size: 6746 ApoI r'AATT_y Cuts at: 1131 1963 2002 2294 3338 3750 6099 6355 1131 Size: 832 39 292 1044 412 2349 256 1522 Fragments arranged by size: 2349 1522 1044 832 412 292 256 39 AseI AT'TA_AT Cuts at: 20 1151 1955 20 Size: 1131 804 4811 Fragments arranged by size: 4811 1131 804 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6746 AvaI C'yCGr_G Cuts at: 190 458 3079 3766 190 Size: 268 2621 687 3170 Fragments arranged by size: 3170 2621 687 268 AvrII C'CTAG_G Cuts at: 163 163 Size: 6746 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2929 2962 3391 3424 2929 Size: 33 429 33 6251 Fragments arranged by size: 6251 429 33 33 BamHI G'GATC_C Cuts at: 593 3771 593 Size: 3178 3568 BanI G'GyrC_C Cuts at: 2256 3699 3762 4176 4424 4459 2256 Size: 1443 63 414 248 35 4543 Fragments arranged by size: 4543 1443 414 248 63 35 BanII G_rGCy'C Cuts at: 191 197 560 1391 3737 3760 4790 191 Size: 6 363 831 2346 23 1030 2147 Fragments arranged by size: 2346 2147 1030 831 363 23 6 BbeI G_GCGC'C Cuts at: 4180 4428 4180 Size: 248 6498 BbsI GAAGACnn'nnnn_ Cuts at: 422 2660 422 Size: 2238 4508 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 3070 3104 90 Size: 34 2946 34 3732 Fragments arranged by size: 3732 2946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4644 5700 6746 4644 Size: 1056 1046 4644 Fragments arranged by size: 4644 1056 1046 BclI T'GATC_A Cuts at: 4266 4266 Size: 6746 BglI GCCn_nnn'nGGC Cuts at: 1314 3057 1314 Size: 1743 5003 BglII A'GATC_T Cuts at: 4261 6298 4261 Size: 2037 4709 BlpI GC'TnA_GC Cuts at: 112 2204 3858 112 Size: 2092 1654 3000 Fragments arranged by size: 3000 2092 1654 Bme1580I G_kGCm'C Cuts at: 1096 2744 4371 4464 5588 1096 Size: 1648 1627 93 1124 2254 Fragments arranged by size: 2254 1648 1627 1124 93 BmgBI CAC'GTC Cuts at: 771 2055 771 Size: 1284 5462 BmrI ACTGGGnnnn_n' Cuts at: 582 2329 2863 3688 3958 4039 4374 6518 582 Size: 1747 534 825 270 81 335 2144 810 Fragments arranged by size: 2144 1747 825 810 534 335 270 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1859 5113 1859 Size: 3254 3492 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 165 205 273 553 837 1214 1310 1831 3255 Size: 40 68 280 284 377 96 521 1424 Cuts at: 3255 165 Size: 3656 Fragments arranged by size: 3656 1424 521 377 284 280 96 68 40 Bpu10I CC'TnA_GC Cuts at: 1304 2706 1304 Size: 1402 5344 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1551 1747 1862 3922 4801 5515 5813 1551 Size: 196 115 2060 879 714 298 2484 Fragments arranged by size: 2484 2060 879 714 298 196 115 BsaI GGTCTCn'nnnn_ Cuts at: 2843 3563 6450 2843 Size: 720 2887 3139 Fragments arranged by size: 3139 2887 720 BsaAI yAC'GTr Cuts at: 608 1917 2754 4099 608 Size: 1309 837 1345 3255 Fragments arranged by size: 3255 1345 1309 837 BsaBI GATnn'nnATC Cuts at: 6 2686 4260 6297 6 Size: 2680 1574 2037 455 Fragments arranged by size: 2680 2037 1574 455 BsaHI Gr'CG_yC Cuts at: 854 2624 3299 4177 4425 5127 6623 854 Size: 1770 675 878 248 702 1496 977 Fragments arranged by size: 1770 1496 977 878 702 675 248 BsaWI w'CCGG_w Cuts at: 590 2678 3023 3314 3935 4456 5134 5545 5692 Size: 2088 345 291 621 521 678 411 147 Cuts at: 5692 5966 590 Size: 274 1370 Fragments arranged by size: 2088 1370 678 621 521 411 345 291 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 526 556 560 571 590 601 2720 2750 3253 Size: 30 4 11 19 11 2119 30 503 Cuts at: 3253 3283 526 Size: 30 3989 Fragments arranged by size: 3989 2119 503 30 30 30 19 11 11 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1471 1744 2233 2623 3403 1471 Size: 273 489 390 780 4814 Fragments arranged by size: 4814 780 489 390 273 BseYI C'CCAG_C Cuts at: 1040 2770 3117 3122 3543 3736 4171 4929 5594 Size: 1730 347 5 421 193 435 758 665 Cuts at: 5594 6002 1040 Size: 408 1784 Fragments arranged by size: 1784 1730 758 665 435 421 408 347 193 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2530 3626 2530 Size: 1096 5650 BsiEI CG_ry'CG Cuts at: 104 3221 3431 4288 4334 5564 104 Size: 3117 210 857 46 1230 1286 Fragments arranged by size: 3117 1286 1230 857 210 46 BsiHKAI G_wGCw'C Cuts at: 197 1391 3760 4538 4728 5588 197 Size: 1194 2369 778 190 860 1355 Fragments arranged by size: 2369 1355 1194 860 778 190 BsiWI C'GTAC_G Cuts at: 3432 3432 Size: 6746 BsmI GAATG_Cn' Cuts at: 2797 2797 Size: 6746 BsmBI CGTCTCn'nnnn_ Cuts at: 845 2639 845 Size: 1794 4952 Bsp1286I G_dGCh'C Cuts at: 191 197 560 1096 1391 2744 3737 3760 4371 Size: 6 363 536 295 1353 993 23 611 Cuts at: 4371 4464 4538 4728 4790 5588 191 Size: 93 74 190 62 798 1349 Fragments arranged by size: 1353 1349 993 798 611 536 363 295 190 93 74 62 23 6 BspEI T'CCGG_A Cuts at: 2678 2678 Size: 6746 BspHI T'CATG_A Cuts at: 867 1929 5178 867 Size: 1062 3249 2435 Fragments arranged by size: 3249 2435 1062 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1843 2252 2591 2605 3312 3796 4010 4312 4693 Size: 409 339 14 707 484 214 302 381 Cuts at: 4693 1843 Size: 3896 Fragments arranged by size: 3896 707 484 409 381 339 302 214 14 BsrBI CCG'CTC Cuts at: 50 1772 5038 5092 6576 6741 50 Size: 1722 3266 54 1484 165 55 Fragments arranged by size: 3266 1722 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 4658 4658 Size: 6746 BsrFI r'CCGG_y Cuts at: 2247 2568 3023 4744 4925 2247 Size: 321 455 1721 181 4068 Fragments arranged by size: 4068 1721 455 321 181 BssHII G'CGCG_C Cuts at: 1474 1474 Size: 6746 BssSI C'ACGA_G Cuts at: 2530 2548 3031 5017 5725 2530 Size: 18 483 1986 708 3551 Fragments arranged by size: 3551 1986 708 483 18 BstAPI GCAn_nnn'nTGC Cuts at: 3654 4123 3654 Size: 469 6277 BstBI TT'CG_AA Cuts at: 5107 5107 Size: 6746 BstEII G'GTnAC_C Cuts at: 3105 3105 Size: 6746 BstXI CCAn_nnnn'nTGG Cuts at: 132 1490 132 Size: 1358 5388 BstYI r'GATC_y Cuts at: 1 593 1797 3771 4238 4261 4596 4842 5148 Size: 592 1204 1974 467 23 335 246 306 Cuts at: 5148 5160 5246 6298 1 Size: 12 86 1052 449 Fragments arranged by size: 1974 1204 1052 592 467 449 335 306 246 86 23 12 BtgI C'CryG_G Cuts at: 432 974 1097 2576 2891 3162 432 Size: 542 123 1479 315 271 4016 Fragments arranged by size: 4016 1479 542 315 271 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4696 4877 5040 4696 Size: 181 163 6402 Fragments arranged by size: 6402 181 163 BtsI GCAGTG_nn' Cuts at: 788 2160 3643 3989 788 Size: 1372 1483 346 3545 Fragments arranged by size: 3545 1483 1372 346 ClaI AT'CG_AT Cuts at: 3465 3465 Size: 6746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 2136 2171 2136 Size: 35 6711 DraI TTT'AAA Cuts at: 1871 3745 1871 Size: 1874 4872 DrdI GACnn_nn'nnGTC Cuts at: 3298 3435 4452 5796 3298 Size: 137 1017 1344 4248 Fragments arranged by size: 4248 1344 1017 137 EaeI y'GGCC_r Cuts at: 1374 1826 2570 2829 3218 4331 4505 4896 4923 Size: 452 744 259 389 1113 174 391 27 Cuts at: 4923 6268 1374 Size: 1345 1852 Fragments arranged by size: 1852 1345 1113 744 452 391 389 259 174 27 EagI C'GGCC_G Cuts at: 3218 4331 3218 Size: 1113 5633 EarI CTCTTCn'nnn_ Cuts at: 838 4769 4979 838 Size: 3931 210 2605 Fragments arranged by size: 3931 2605 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 169 4954 5696 5842 6556 169 Size: 4785 742 146 714 359 Fragments arranged by size: 4785 742 714 359 146 EcoICRI GAG'CTC Cuts at: 195 1389 3758 195 Size: 1194 2369 3183 Fragments arranged by size: 3183 2369 1194 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 165 205 273 553 837 922 1214 1310 1831 Size: 40 68 280 284 85 292 96 521 Cuts at: 1831 3255 4571 5003 5356 6018 165 Size: 1424 1316 432 353 662 893 Fragments arranged by size: 1424 1316 893 662 521 432 353 292 284 280 96 85 68 40 EcoNI CCTnn'n_nnAGG Cuts at: 490 3140 490 Size: 2650 4096 EcoO109I rG'GnC_Cy Cuts at: 1815 2241 3036 3234 3885 1815 Size: 426 795 198 651 4676 Fragments arranged by size: 4676 795 651 426 198 EcoRI G'AATT_C Cuts at: 1131 3338 3750 1131 Size: 2207 412 4127 Fragments arranged by size: 4127 2207 412 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3162 4765 3162 Size: 1603 5143 FspI TGC'GCA Cuts at: 4527 4527 Size: 6746 HaeII r_GCGC'y Cuts at: 826 4180 4428 5658 6429 826 Size: 3354 248 1230 771 1143 Fragments arranged by size: 3354 1230 1143 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1120 1152 1883 1915 2642 2674 4588 4618 4620 Size: 32 731 32 727 32 1914 30 2 Cuts at: 4620 4650 1120 Size: 30 3216 Fragments arranged by size: 3216 1914 731 727 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1520 3785 1520 Size: 2265 4481 HindIII A'AGCT_T Cuts at: 1498 3527 1498 Size: 2029 4717 HpaI GTT'AAC Cuts at: 1520 1520 Size: 6746 KasI G'GCGC_C Cuts at: 4176 4424 4176 Size: 248 6498 KpnI G_GTAC'C Cuts at: 3766 3766 Size: 6746 MluI A'CGCG_T Cuts at: 6179 6179 Size: 6746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 306 1175 2470 2537 2798 3357 3512 5505 5689 Size: 869 1295 67 261 559 155 1993 184 Cuts at: 5689 306 Size: 1363 Fragments arranged by size: 1993 1363 1295 869 559 261 184 155 67 MscI TGG'CCA Cuts at: 2831 4507 2831 Size: 1676 5070 MslI CAynn'nnrTG Cuts at: 628 884 2066 2752 3103 4735 4862 628 Size: 256 1182 686 351 1632 127 2512 Fragments arranged by size: 2512 1632 1182 686 351 256 127 MspA1I CmG'CkG Cuts at: 183 1025 1379 3857 4171 4531 5315 5560 183 Size: 842 354 2478 314 360 784 245 1369 Fragments arranged by size: 2478 1369 842 784 360 354 314 245 NaeI GCC'GGC Cuts at: 2570 4927 2570 Size: 2357 4389 NarI GG'CG_CC Cuts at: 4177 4425 4177 Size: 248 6498 NcoI C'CATG_G Cuts at: 432 974 2576 2891 3162 432 Size: 542 1602 315 271 4016 Fragments arranged by size: 4016 1602 542 315 271 NgoMIV G'CCGG_C Cuts at: 2568 4925 2568 Size: 2357 4389 NspI r_CATG'y Cuts at: 1689 1897 2699 3799 4299 5902 1689 Size: 208 802 1100 500 1603 2533 Fragments arranged by size: 2533 1603 1100 802 500 208 PasI CC'CwG_GG Cuts at: 1819 1819 Size: 6746 PciI A'CATG_T Cuts at: 1685 5898 1685 Size: 4213 2533 PflMI CCAn_nnn'nTGG Cuts at: 1074 1074 Size: 6746 PmeI GTTT'AAAC Cuts at: 3745 3745 Size: 6746 PmlI CAC'GTG Cuts at: 608 2754 608 Size: 2146 4600 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5192 5224 5192 Size: 32 6714 PpuMI rG'GwC_Cy Cuts at: 1815 2241 3234 1815 Size: 426 993 5327 Fragments arranged by size: 5327 993 426 PshAI GACnn'nnGTC Cuts at: 2390 2390 Size: 6746 PspXI vC'TCGA_Gb Cuts at: 3079 3079 Size: 6746 PstI C_TGCA'G Cuts at: 253 2271 2602 3650 3793 4478 253 Size: 2018 331 1048 143 685 2521 Fragments arranged by size: 2521 2018 1048 685 331 143 PvuI CG_AT'CG Cuts at: 104 104 Size: 6746 PvuII CAG'CTG Cuts at: 183 1025 4171 4531 183 Size: 842 3146 360 2398 Fragments arranged by size: 3146 2398 842 360 RsrII CG'GwC_CG Cuts at: 4941 4941 Size: 6746 SacI G_AGCT'C Cuts at: 197 1391 3760 197 Size: 1194 2369 3183 Fragments arranged by size: 3183 2369 1194 SalI G'TCGA_C Cuts at: 3783 3783 Size: 6746 SapI GCTCTTCn'nnn_ Cuts at: 4769 4979 4769 Size: 210 6536 SbfI CC_TGCA'GG Cuts at: 2602 3793 2602 Size: 1191 5555 ScaI AGT'ACT Cuts at: 6263 6263 Size: 6746 SfcI C'TryA_G Cuts at: 33 249 1535 2267 2563 2598 3646 3789 4474 Size: 216 1286 732 296 35 1048 143 685 Cuts at: 4474 5442 5633 33 Size: 968 191 1146 Fragments arranged by size: 1286 1146 1048 968 732 685 296 216 191 143 35 SfoI GGC'GCC Cuts at: 4178 4426 4178 Size: 248 6498 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6746 SmaI CCC'GGG Cuts at: 3768 3768 Size: 6746 SmlI C'TyrA_G Cuts at: 1566 1726 1782 1877 3079 3901 4816 5530 5792 Size: 160 56 95 1202 822 915 714 262 Cuts at: 5792 1566 Size: 2520 Fragments arranged by size: 2520 1202 915 822 714 262 160 95 56 SpeI A'CTAG_T Cuts at: 67 67 Size: 6746 SphI G_CATG'C Cuts at: 3799 4299 3799 Size: 500 6246 SspI AAT'ATT Cuts at: 1149 1149 Size: 6746 StuI AGG'CCT Cuts at: 1839 1839 Size: 6746 StyI C'CwwG_G Cuts at: 163 432 722 974 2576 2891 3162 3880 4234 Size: 269 290 252 1602 315 271 718 354 Cuts at: 4234 163 Size: 2675 Fragments arranged by size: 2675 1602 718 354 315 290 271 269 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 564 4329 4462 4921 5130 564 Size: 3765 133 459 209 2180 Fragments arranged by size: 3765 2180 459 209 133 TatI w'GTAC_w Cuts at: 1883 6261 1883 Size: 4378 2368 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 320 352 526 558 560 592 2364 2396 2423 Size: 32 174 32 2 32 1772 32 27 Cuts at: 2423 2455 3253 3285 6263 6295 320 Size: 32 798 32 2978 32 771 Fragments arranged by size: 2978 1772 798 771 174 32 32 32 32 32 32 32 27 2 Tth111I GACn'n_nGTC Cuts at: 661 772 2033 2626 4543 661 Size: 111 1261 593 1917 2864 Fragments arranged by size: 2864 1917 1261 593 111 XbaI T'CTAG_A Cuts at: 3777 3777 Size: 6746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2206 2889 4241 2206 Size: 683 1352 4711 Fragments arranged by size: 4711 1352 683 XhoI C'TCGA_G Cuts at: 3079 3079 Size: 6746 XmaI C'CCGG_G Cuts at: 3766 3766 Size: 6746 XmnI GAAnn'nnTTC Cuts at: 3145 3145 Size: 6746 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AfeI AhdI AlfI ApaI AscI BbvCI BfrBI BmtI BsrGI BstZ17I Bsu36I DraIII EcoRV FseI FspAI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI