Restriction map for pF1KB9575
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvrII       BanI        BbeI        BbrPI       
BbvCI       BciVI       BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaHI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspHI       BspLU11I    
BspMI       BsrFI       BsrGI       BssHII      BstBI       
CelII       Cfr10I      CpoI        DraI        DraIII      
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       FseI        HaeII       
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MfeI        MluI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccIII       1586  TCCGGA               T^CCGGA
Alw44I       1420  GTGCAC               G^TGCAC
AlwNI        1453  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1420  GTGCAC               G^TGCAC
Asp700I        17  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1325  TGCGCA               TGC^GCA
BamHI        1589  GGATCC               G^GATCC
BanII        1441  GAGCCC               G[GA]GC[CT]^C
BcgI          639  CGATGGTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          281  TGATCA               T^GATCA
BglI         1546  GCCAAGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1188  CACGTA               [CT]AC^GT[GA]
BsaBI        1710  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1815  GAGACC               GGTCTC(1/5)
BseAI        1586  TCCGGA               T^CCGGA
BsgI          967  CTGCAC               GTGCAG(16/14)
BsmI         1283  GCATTC               GAATGC(1/-1)
BspDI         688  ATCGAT               AT^CGAT
BspEI        1586  TCCGGA               T^CCGGA
BsrBI          85  CCGCTC               CCGCTC(-3/-3)
BssSI        1627  CTCGTG               CACGAG(-5/-1)
Bst1107I      865  GTATAC               GTA^TAC
BstAPI       1230  GCATGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1535  CCATGCCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       865  GTATAC               GTA^TAC
ClaI          688  ATCGAT               AT^CGAT
EcoNI          12  CCTCGGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1228  ATGCAT               ATGCA^T
FbaI          281  TGATCA               T^GATCA
FspI         1325  TGCGCA               TGC^GCA
HincII       1684  GTTGAC               GT[CT]^[GA]AC
HindII       1684  GTTGAC               GT[CT]^[GA]AC
MamI         1710  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1586  TCCGGA               T^CCGGA
NsiI         1228  ATGCAT               ATGCA^T
PleI          154  GAGTC                GAGTC(4/5)
PstI         1450  CTGCAG               CTGCA^G
StuI            9  AGGCCT               AGG^CCT
XmnI           17  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          832  GTAGAC               GT^[AC][GT]AC
              865  GTATAC              
AccII          79  CGCG                 CG^CG
             1719  CGCG                
AcsI         1663  GAATTT               [GA]^AATT[CT]
             1774  AAATTT              
AocI           65  CCTCAGG              CC^T[ACGT]AGG
              274  CCTGAGG             
ApoI         1663  GAATTT               [GA]^AATT[CT]
             1774  AAATTT              
AspHI         109  GTGCTC               G[AT]GC[AT]^C
             1420  GTGCAC              
AvaI           42  CCCGAG               C^[CT]CG[GA]G
              466  CCCGAG              
BcnI           33  CCCGG                CC^[GC]GG
             1128  CCGGG               
BsaWI          25  TCCGGT               [AT]^CCGG[AT]
             1586  TCCGGA              
BsiHKAI       109  GTGCTC               G[AT]GC[AT]^C
             1420  GTGCAC              
BsoBI          42  CCCGAG               C^[CT]CG[GA]G
              466  CCCGAG              
BstUI          79  CGCG                 CG^CG
             1719  CGCG                
Bsu36I         65  CCTCAGG              CC^T[ACGT]AGG
              274  CCTGAGG             
DraII         224  AGGACCT              [GA]G^G[ACGT]CC[CT]
              567  GGGACCT             
DrdI         1342  GACCCACAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1723  GACACCAAGGTC        
EarI          616  CTCTTC               CTCTTC(1/4)
             1032  GAAGAG              
Eco81I         65  CCTCAGG              CC^T[ACGT]AGG
              274  CCTGAGG             
EcoO109I      224  AGGACCT              [GA]G^G[ACGT]CC[CT]
              567  GGGACCT             
HgaI           71  GACGC                GACGC(5/10)
             1107  GCGTC               
Ksp632I       616  CTCTTC               CTCTTC(1/4)
             1032  GAAGAG              
MaeII         240  ACGT                 A^CGT
             1189  ACGT                
MvnI           79  CGCG                 CG^CG
             1719  CGCG                
NciI           33  CCCGG                CC^[GC]GG
             1128  CCGGG               
NlaIV         567  GGGACC               GG[ACGT]^[ACGT]CC
             1589  GGATCC              
PpuMI         224  AGGACCT              [GA]G^G[AT]CC[CT]
              567  GGGACCT             
SphI         1230  GCATGC               GCATG^C
             1328  GCATGC              
TfiI          443  GATTC                G^A[AT]TC
              592  GAATC               
XcmI          998  CCATCAAGGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1532  CCACCATGCCTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BalI        BbsI        BbvI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstEII      BstF5I      BstNI       BstPI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EaeI        EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NspI        PflMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
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