################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI HphI KasI KpnI KspI MaeII MamI MluI MroI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 51 CGCG CG^CG AlwI 533 GGATC GGATC(4/5) AlwNI 350 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 79 GGTCC G^G[AT]CC BalI 998 TGGCCA TGG^CCA BbsI 726 GTCTTC GAAGAC(2/6) BciVI 278 GGATAC GTATCC(6/5) BcnI 76 CCCGG CC^[GC]GG BlpI 649 GCTAAGC GC^T[ACGT]AGC BmyI 416 GTGCCC G[AGT]GC[ACT]^C Bpu1102I 649 GCTAAGC GC^T[ACGT]AGC BpuAI 726 GTCTTC GAAGAC(2/6) BsaJI 8 CCTCGG C^C[ACGT][ACGT]GG BsiYI 15 CCCTGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 15 CCCTGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 80 GTCCC GGGAC(10/14) Bsp1286I 416 GTGCCC G[AGT]GC[ACT]^C BsrDI 575 CATTGC GCAATG(2/0) BstF5I 553 CATCC GGATG(2/0) BstNI 349 CCAGG CC^[AT]GG BstUI 51 CGCG CG^CG BstYI 533 GGATCT [GA]^GATC[CT] Cac8I 48 GCCCGC GC[ACGT]^[ACGT]GC CelII 649 GCTAAGC GC^T[ACGT]AGC EaeI 998 TGGCCA [CT]^GGCC[GA] EcoRII 349 CCAGG ^CC[AT]GG EcoT22I 550 ATGCAT ATGCA^T FauI 49 CCCGC CCCGC(4/6) FokI 553 CATCC GGATG(9/13) HapII 77 CCGG C^CGG HgaI 52 GCGTC GACGC(5/10) HincII 195 GTTGAC GT[CT]^[GA]AC HindII 195 GTTGAC GT[CT]^[GA]AC HpaII 77 CCGG C^CGG MaeIII 1021 GTCAC ^GT[ACGT]AC MfeI 236 CAATTG C^AATTG MflI 533 GGATCT [GA]^GATC[CT] MluNI 998 TGGCCA TGG^CCA MscI 998 TGGCCA TGG^CCA MspI 77 CCGG C^CGG MunI 236 CAATTG C^AATTG MvaI 349 CCAGG CC^[AT]GG MvnI 51 CGCG CG^CG NciI 76 CCCGG CC^[GC]GG NlaIII 465 CATG CATG^ NsiI 550 ATGCAT ATGCA^T PacI 934 TTAATTAA TTAAT^TAA SfcI 959 CTACAG C^T[GA][CT]AG SpeI 522 ACTAGT A^CTAGT Tsp45I 1021 GTCAC ^GT[GC]AC XcmI 1001 CCAGTGCTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 533 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AluI 804 AGCT AG^CT 1115 AGCT BbvI 860 GCAGC GCAGC(8/12) 19 GCTGC BfaI 523 CTAG C^TAG 689 CTAG BsmAI 152 GTCTC GTCTC(1/5) 208 GTCTC BsrI 996 ACTGG ACTGG(1/-1) 1001 CCAGT BssKI 76 CCCGG ^CC[ACGT]GG 349 CCAGG Cfr13I 12 GGCCC G^G[ACGT]CC 79 GGTCC DpnII 503 GATC ^GATC 534 GATC Fnu4HI 19 GCTGC GC^[ACGT]GC 860 GCAGC ItaI 19 GCTGC GC^[ACGT]GC 860 GCAGC MaeI 523 CTAG C^TAG 689 CTAG MboI 503 GATC ^GATC 534 GATC NdeII 503 GATC ^GATC 534 GATC Sau3AI 503 GATC ^GATC 534 GATC Sau96I 12 GGCCC G^G[ACGT]CC 79 GGTCC ScrFI 76 CCCGG CC^[ACGT]GG 349 CCAGG SfaNI 476 GCATC GCATC(5/9) 552 GCATC TfiI 685 GAATC G^A[AT]TC 876 GAATC TseI 19 GCTGC G^C[AT]GC 860 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI AcsI ApoI BseRI DdeI EarI HaeIII HinfI Ksp632I MboII MnlI MseI MwoI NlaIV PleI Tru9I Tsp509I TspRI