(Circular) (Six-base) MAPSORT of: pF1KB9572.seq Check: 4874 from: 1 to: 5477 pF1KB9572 5477 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AccI GT'mk_AC Cuts at: 2515 2515 Size: 5477 Acc65I G'GTAC_C Cuts at: 2493 2493 Size: 5477 AclI AA'CG_TT Cuts at: 5317 5317 Size: 5477 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 299 320 443 888 932 2115 3302 3734 4481 Size: 21 123 445 44 1183 1187 432 747 Cuts at: 4481 4749 299 Size: 268 1027 Fragments arranged by size: 1187 1183 1027 747 445 432 268 123 44 21 AflIII A'CryG_T Cuts at: 2829 3933 4910 2829 Size: 1104 977 3396 Fragments arranged by size: 3396 1104 977 AgeI A'CCGG_T Cuts at: 3865 3865 Size: 5477 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 670 670 Size: 5477 AlwNI CAG_nnn'CTG Cuts at: 132 367 739 2064 2088 2253 2782 4349 132 Size: 235 372 1325 24 165 529 1567 1260 Fragments arranged by size: 1567 1325 1260 529 372 235 165 24 ApaI G_GGCC'C Cuts at: 212 2394 212 Size: 2182 3295 ApaLI G'TGCA_C Cuts at: 4247 4247 Size: 5477 ApoI r'AATT_y Cuts at: 507 542 1516 2481 4830 5086 507 Size: 35 974 965 2349 256 898 Fragments arranged by size: 2349 974 965 898 256 35 AseI AT'TA_AT Cuts at: 20 20 Size: 5477 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5477 AvaI C'yCGr_G Cuts at: 211 223 721 1592 2497 211 Size: 12 498 871 905 3191 Fragments arranged by size: 3191 905 871 498 12 BamHI G'GATC_C Cuts at: 1386 2502 1386 Size: 1116 4361 BanI G'GyrC_C Cuts at: 1702 2493 2907 3155 3190 1702 Size: 791 414 248 35 3989 Fragments arranged by size: 3989 791 414 248 35 BanII G_rGCy'C Cuts at: 212 1054 1348 1831 2394 2491 3521 212 Size: 842 294 483 563 97 1030 2168 Fragments arranged by size: 2168 1030 842 563 483 294 97 BbeI G_GCGC'C Cuts at: 2911 3159 2911 Size: 248 5229 BbsI GAAGACnn'nnnn_ Cuts at: 1757 1757 Size: 5477 BbvCI CC'TCA_GC Cuts at: 1849 1849 Size: 5477 BciVI GTATCCnnnnn_n' Cuts at: 3375 4136 5477 3375 Size: 761 1341 3375 Fragments arranged by size: 3375 1341 761 BclI T'GATC_A Cuts at: 2997 2997 Size: 5477 BfrBI ATG'CAT Cuts at: 1585 1585 Size: 5477 BglII A'GATC_T Cuts at: 2992 5029 2992 Size: 2037 3440 BlpI GC'TnA_GC Cuts at: 2589 2589 Size: 5477 Bme1580I G_kGCm'C Cuts at: 212 779 877 2394 3102 3195 4251 212 Size: 567 98 1517 708 93 1056 1438 Fragments arranged by size: 1517 1438 1056 708 567 98 93 BmgBI CAC'GTC Cuts at: 161 1679 1733 161 Size: 1518 54 3905 Fragments arranged by size: 3905 1518 54 BmrI ACTGGGnnnn_n' Cuts at: 1150 1210 2689 2770 3105 5249 1150 Size: 60 1479 81 335 2144 1378 Fragments arranged by size: 2144 1479 1378 335 81 60 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1073 2354 3844 1073 Size: 1281 1490 2706 Fragments arranged by size: 2706 1490 1281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 664 1798 664 Size: 1134 4343 Bpu10I CC'TnA_GC Cuts at: 1849 2204 2303 1849 Size: 355 99 5023 Fragments arranged by size: 5023 355 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 279 1045 1087 2653 3532 4024 4322 279 Size: 766 42 1566 879 492 298 1434 Fragments arranged by size: 1566 1434 879 766 492 298 42 BsaI GGTCTCn'nnnn_ Cuts at: 241 1939 5181 241 Size: 1698 3242 537 Fragments arranged by size: 3242 1698 537 BsaAI yAC'GTr Cuts at: 2830 2830 Size: 5477 BsaBI GATnn'nnATC Cuts at: 6 2991 5028 6 Size: 2985 2037 455 Fragments arranged by size: 2985 2037 455 BsaHI Gr'CG_yC Cuts at: 674 2908 3156 3858 5354 674 Size: 2234 248 702 1496 797 Fragments arranged by size: 2234 1496 797 702 248 BsaWI w'CCGG_w Cuts at: 2666 3187 3865 4139 4286 4697 2666 Size: 521 678 274 147 411 3446 Fragments arranged by size: 3446 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1140 1170 1140 Size: 30 5447 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 241 244 854 1054 1062 1675 2248 241 Size: 3 610 200 8 613 573 3470 Fragments arranged by size: 3470 613 610 573 200 8 3 BseYI C'CCAG_C Cuts at: 778 1032 1282 2457 2902 3660 4237 4733 778 Size: 254 250 1175 445 758 577 496 1522 Fragments arranged by size: 1522 1175 758 577 496 445 254 250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 163 2354 163 Size: 2191 3286 BsiEI CG_ry'CG Cuts at: 104 3019 3065 4273 104 Size: 2915 46 1208 1308 Fragments arranged by size: 2915 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1054 1877 2205 2491 3269 3459 4251 1054 Size: 823 328 286 778 190 792 2280 Fragments arranged by size: 2280 823 792 778 328 286 190 BsmBI CGTCTCn'nnnn_ Cuts at: 1220 1723 1220 Size: 503 4974 Bsp1286I G_dGCh'C Cuts at: 212 779 877 1054 1348 1831 1877 2205 2394 Size: 567 98 177 294 483 46 328 189 Cuts at: 2394 2491 3102 3195 3269 3459 3521 4251 212 Size: 97 611 93 74 190 62 730 1438 Fragments arranged by size: 1438 730 611 567 483 328 294 190 189 177 98 97 93 74 62 46 BspEI T'CCGG_A Cuts at: 4697 4697 Size: 5477 BspHI T'CATG_A Cuts at: 4653 4653 Size: 5477 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1183 2527 2741 3043 3424 1183 Size: 1344 214 302 381 3236 Fragments arranged by size: 3236 1344 381 302 214 BsrBI CCG'CTC Cuts at: 50 3769 3823 5307 5472 50 Size: 3719 54 1484 165 55 Fragments arranged by size: 3719 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3389 3389 Size: 5477 BsrFI r'CCGG_y Cuts at: 187 3475 3656 3865 187 Size: 3288 181 209 1799 Fragments arranged by size: 3288 1799 209 181 BssSI C'ACGA_G Cuts at: 1223 3748 4106 1223 Size: 2525 358 2594 Fragments arranged by size: 2594 2525 358 BstAPI GCAn_nnn'nTGC Cuts at: 2854 2854 Size: 5477 BstBI TT'CG_AA Cuts at: 2310 3838 2310 Size: 1528 3949 BstEII G'GTnAC_C Cuts at: 714 714 Size: 5477 BstYI r'GATC_y Cuts at: 1 128 1260 1386 1841 2502 2969 2992 3327 Size: 127 1132 126 455 661 467 23 335 Cuts at: 3327 3573 4585 4671 4683 5029 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1132 1012 661 467 455 449 346 335 246 127 126 86 23 12 Bsu36I CC'TnA_GG Cuts at: 820 820 Size: 5477 BtgI C'CryG_G Cuts at: 107 121 584 1382 2069 107 Size: 14 463 798 687 3515 Fragments arranged by size: 3515 798 687 463 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 706 3427 3608 3771 706 Size: 2721 181 163 2412 Fragments arranged by size: 2721 2412 181 163 BtsI GCAGTG_nn' Cuts at: 151 1425 1613 2720 151 Size: 1274 188 1107 2908 Fragments arranged by size: 2908 1274 1107 188 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1749 1784 1749 Size: 35 5442 DraI TTT'AAA Cuts at: 1395 2476 1395 Size: 1081 4396 DrdI GACnn_nn'nnGTC Cuts at: 3183 4041 3183 Size: 858 4619 EaeI y'GGCC_r Cuts at: 1644 3062 3236 3627 3654 4999 1644 Size: 1418 174 391 27 1345 2122 Fragments arranged by size: 2122 1418 1345 391 174 27 EagI C'GGCC_G Cuts at: 3062 3062 Size: 5477 EarI CTCTTCn'nnn_ Cuts at: 908 1341 1886 1937 2044 2134 2264 2288 3500 Size: 433 545 51 107 90 130 24 1212 Cuts at: 3500 3710 908 Size: 210 2675 Fragments arranged by size: 2675 1212 545 433 210 130 107 90 51 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3685 3995 4141 5287 3685 Size: 310 146 1146 3875 Fragments arranged by size: 3875 1146 310 146 EcoICRI GAG'CTC Cuts at: 1052 2489 1052 Size: 1437 4040 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 299 320 443 664 888 932 1798 2115 3302 Size: 21 123 221 224 44 866 317 1187 Cuts at: 3302 3734 4481 4749 299 Size: 432 747 268 1027 Fragments arranged by size: 1187 1027 866 747 432 317 268 224 221 123 44 21 EcoNI CCTnn'n_nnAGG Cuts at: 941 941 Size: 5477 EcoO109I rG'GnC_Cy Cuts at: 364 1797 2085 2114 2250 2390 2391 2616 364 Size: 1433 288 29 136 140 1 225 3225 Fragments arranged by size: 3225 1433 288 225 140 136 29 1 EcoRI G'AATT_C Cuts at: 2481 2481 Size: 5477 EcoRV GAT'ATC Cuts at: 591 591 Size: 5477 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3496 3496 Size: 5477 FspI TGC'GCA Cuts at: 3258 3258 Size: 5477 HaeII r_GCGC'y Cuts at: 2911 3159 4181 5160 2911 Size: 248 1022 979 3228 Fragments arranged by size: 3228 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 238 270 481 513 1989 2021 2086 2118 3319 Size: 32 211 32 1476 32 65 32 1201 Cuts at: 3319 3349 3351 3381 238 Size: 30 2 30 2334 Fragments arranged by size: 2334 1476 1201 211 65 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2516 2516 Size: 5477 HindIII A'AGCT_T Cuts at: 1063 1126 1063 Size: 63 5414 KasI G'GCGC_C Cuts at: 2907 3155 2907 Size: 248 5229 KpnI G_GTAC'C Cuts at: 2497 2497 Size: 5477 MfeI C'AATT_G Cuts at: 833 833 Size: 5477 MluI A'CGCG_T Cuts at: 4910 4910 Size: 5477 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 899 4148 4332 899 Size: 3249 184 2044 Fragments arranged by size: 3249 2044 184 MscI TGG'CCA Cuts at: 1646 3238 1646 Size: 1592 3885 MslI CAynn'nnrTG Cuts at: 626 1451 3466 3593 626 Size: 825 2015 127 2510 Fragments arranged by size: 2510 2015 825 127 MspA1I CmG'CkG Cuts at: 1699 1870 2588 2902 3262 4275 4520 1699 Size: 171 718 314 360 1013 245 2656 Fragments arranged by size: 2656 1013 718 360 314 245 171 NaeI GCC'GGC Cuts at: 189 3658 189 Size: 3469 2008 NarI GG'CG_CC Cuts at: 2908 3156 2908 Size: 248 5229 NcoI C'CATG_G Cuts at: 107 1382 2069 107 Size: 1275 687 3515 Fragments arranged by size: 3515 1275 687 NgoMIV G'CCGG_C Cuts at: 187 3656 187 Size: 3469 2008 NsiI A_TGCA'T Cuts at: 1587 1587 Size: 5477 NspI r_CATG'y Cuts at: 1589 2530 3030 3937 1589 Size: 941 500 907 3129 Fragments arranged by size: 3129 941 907 500 PciI A'CATG_T Cuts at: 3933 3933 Size: 5477 PflMI CCAn_nnn'nTGG Cuts at: 1743 1743 Size: 5477 PfoI T'CCnGG_A Cuts at: 680 680 Size: 5477 PmeI GTTT'AAAC Cuts at: 2476 2476 Size: 5477 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 4616 Size: 32 5445 PpuMI rG'GwC_Cy Cuts at: 364 2114 364 Size: 1750 3727 PspOMI G'GGCC_C Cuts at: 208 2390 208 Size: 2182 3295 PspXI vC'TCGA_Gb Cuts at: 721 721 Size: 5477 PstI C_TGCA'G Cuts at: 639 1319 1791 2399 2524 3209 639 Size: 680 472 608 125 685 2907 Fragments arranged by size: 2907 685 680 608 472 125 PvuI CG_AT'CG Cuts at: 104 104 Size: 5477 PvuII CAG'CTG Cuts at: 1699 1870 2902 3262 1699 Size: 171 1032 360 3914 Fragments arranged by size: 3914 1032 360 171 RsrII CG'GwC_CG Cuts at: 3672 3672 Size: 5477 SacI G_AGCT'C Cuts at: 1054 2491 1054 Size: 1437 4040 SalI G'TCGA_C Cuts at: 2514 2514 Size: 5477 SapI GCTCTTCn'nnn_ Cuts at: 2044 3500 3710 2044 Size: 1456 210 3811 Fragments arranged by size: 3811 1456 210 SbfI CC_TGCA'GG Cuts at: 2524 2524 Size: 5477 ScaI AGT'ACT Cuts at: 4994 4994 Size: 5477 SfcI C'TryA_G Cuts at: 33 635 1208 1315 1787 2395 2520 3205 4198 Size: 602 573 107 472 608 125 685 993 Cuts at: 4198 4389 33 Size: 191 1121 Fragments arranged by size: 1121 993 685 608 602 573 472 191 125 107 SfoI GGC'GCC Cuts at: 2909 3157 2909 Size: 248 5229 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5477 SgrAI Cr'CCGG_yG Cuts at: 187 187 Size: 5477 SmaI CCC'GGG Cuts at: 2499 2499 Size: 5477 SmlI C'TyrA_G Cuts at: 258 721 1060 1066 2632 3547 4039 4301 258 Size: 463 339 6 1566 915 492 262 1434 Fragments arranged by size: 1566 1434 915 492 463 339 262 6 SpeI A'CTAG_T Cuts at: 67 67 Size: 5477 SphI G_CATG'C Cuts at: 2530 3030 2530 Size: 500 4977 StuI AGG'CCT Cuts at: 727 727 Size: 5477 StyI C'CwwG_G Cuts at: 107 887 1120 1382 2069 2611 2965 107 Size: 780 233 262 687 542 354 2619 Fragments arranged by size: 2619 780 687 542 354 262 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1485 3060 3193 3652 3861 1485 Size: 1575 133 459 209 3101 Fragments arranged by size: 3101 1575 459 209 133 TatI w'GTAC_w Cuts at: 4992 4992 Size: 5477 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1140 1172 4994 5026 1140 Size: 32 3822 32 1591 Fragments arranged by size: 3822 1591 32 32 Tth111I GACn'n_nGTC Cuts at: 162 3274 162 Size: 3112 2365 XbaI T'CTAG_A Cuts at: 2508 2508 Size: 5477 XcmI CCAnnnn_n'nnnnTGG Cuts at: 864 2972 864 Size: 2108 3369 XhoI C'TCGA_G Cuts at: 721 721 Size: 5477 XmaI C'CCGG_G Cuts at: 2497 2497 Size: 5477 XmnI GAAnn'nnTTC Cuts at: 2053 2053 Size: 5477 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BcgI BglI BmtI BsiWI BsmI BsrGI BssHII BstXI BstZ17I ClaI DraIII FseI FspAI HpaI NdeI NheI NotI NruI PacI PasI PmlI PshAI PsiI PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI SwaI ZraI