Restriction map for
pF1KB9569
(Circular) (Six-base) MAPSORT of: pF1KB9569.seq Check: 5121 from: 1 to: 5336
pF1KB9569 5336 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 2374 2374
Size: 5336
Acc65I G'GTAC_C
Cuts at: 2352 2352
Size: 5336
AclI AA'CG_TT
Cuts at: 5176 5176
Size: 5336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 1047 1577 3161 3593 3946 4608 359
Size: 688 530 1584 432 353 662 1087
Fragments arranged by size:
1584 1087 688 662 530 432 353
AflIII A'CryG_T
Cuts at: 1115 2688 4488 4769 1115
Size: 1573 1800 281 1682
Fragments arranged by size:
1800 1682 1573 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 557 560 557
Size: 3 5333
AlwNI CAG_nnn'CTG
Cuts at: 498 1822 1825 1885 2197 2641 4079 498
Size: 1324 3 60 312 444 1438 1755
Fragments arranged by size:
1755 1438 1324 444 312 60 3
ApaLI G'TGCA_C
Cuts at: 4174 4174
Size: 5336
ApoI r'AATT_y
Cuts at: 1206 2340 4689 4945 1206
Size: 1134 2349 256 1597
Fragments arranged by size:
2349 1597 1134 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5336
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5336
AvaI C'yCGr_G
Cuts at: 2356 2356
Size: 5336
BamHI G'GATC_C
Cuts at: 2361 2361
Size: 5336
BanI G'GyrC_C
Cuts at: 1911 1936 2352 2766 3014 3049 1911
Size: 25 416 414 248 35 4198
Fragments arranged by size:
4198 416 414 248 35 25
BanII G_rGCy'C
Cuts at: 947 1034 2103 2350 3380 947
Size: 87 1069 247 1030 2903
Fragments arranged by size:
2903 1069 1030 247 87
BbeI G_GCGC'C
Cuts at: 2770 3018 2770
Size: 248 5088
BbsI GAAGACnn'nnnn_
Cuts at: 118 118
Size: 5336
BbvCI CC'TCA_GC
Cuts at: 788 1102 1650 1692 788
Size: 314 548 42 4432
Fragments arranged by size:
4432 548 314 42
BciVI GTATCCnnnnn_n'
Cuts at: 3234 4290 5336 3234
Size: 1056 1046 3234
Fragments arranged by size:
3234 1056 1046
BclI T'GATC_A
Cuts at: 1341 2856 1341
Size: 1515 3821
BglI GCCn_nnn'nGGC
Cuts at: 2293 2323 2293
Size: 30 5306
BglII A'GATC_T
Cuts at: 125 2851 4888 125
Size: 2726 2037 573
Fragments arranged by size:
2726 2037 573
BlpI GC'TnA_GC
Cuts at: 2448 2448
Size: 5336
Bme1580I G_kGCm'C
Cuts at: 1203 2961 3054 4178 1203
Size: 1758 93 1124 2361
Fragments arranged by size:
2361 1758 1124 93
BmgBI CAC'GTC
Cuts at: 1661 1661
Size: 5336
BmrI ACTGGGnnnn_n'
Cuts at: 440 941 2078 2548 2629 2964 5108 440
Size: 501 1137 470 81 335 2144 668
Fragments arranged by size:
2144 1137 668 501 470 335 81
BmtI G_CTAG'C
Cuts at: 415 415
Size: 5336
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1185 1991 3703 1185
Size: 806 1712 2818
Fragments arranged by size:
2818 1712 806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 454 569 1173 1196 1625 1952 437
Size: 17 115 604 23 429 327 3821
Fragments arranged by size:
3821 604 429 327 115 23 17
Bpu10I CC'TnA_GC
Cuts at: 788 1102 1650 1692 1966 788
Size: 314 548 42 274 4158
Fragments arranged by size:
4158 548 314 274 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 985 1248 2512 3391 4105 4403 976
Size: 9 263 1264 879 714 298 1909
Fragments arranged by size:
1909 1264 879 714 298 263 9
BsaI GGTCTCn'nnnn_
Cuts at: 757 1255 1720 2184 5040 757
Size: 498 465 464 2856 1053
Fragments arranged by size:
2856 1053 498 465 464
BsaAI yAC'GTr
Cuts at: 2689 2689
Size: 5336
BsaBI GATnn'nnATC
Cuts at: 6 124 2850 4887 6
Size: 118 2726 2037 455
Fragments arranged by size:
2726 2037 455 118
BsaHI Gr'CG_yC
Cuts at: 445 733 2767 3015 3717 5213 445
Size: 288 2034 248 702 1496 568
Fragments arranged by size:
2034 1496 702 568 288 248
BsaWI w'CCGG_w
Cuts at: 2525 3046 3724 4135 4282 4556 2525
Size: 521 678 411 147 274 3305
Fragments arranged by size:
3305 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1588 1618 1623 1653 1737 1767 2221 2251 1588
Size: 30 5 30 84 30 454 30 4673
Fragments arranged by size:
4673 454 84 30 30 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 224 955 973 2111 224
Size: 731 18 1138 3449
Fragments arranged by size:
3449 1138 731 18
BseYI C'CCAG_C
Cuts at: 365 401 452 842 931 1465 1983 2761 3519
Size: 36 51 390 89 534 518 778 758
Cuts at: 3519 4184 4592 365
Size: 665 408 1109
Fragments arranged by size:
1109 778 758 665 534 518 408 390
89 51 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 532 1535 1826 2195 400
Size: 132 1003 291 369 3541
Fragments arranged by size:
3541 1003 369 291 132
BsiEI CG_ry'CG
Cuts at: 104 2878 2924 4154 104
Size: 2774 46 1230 1286
Fragments arranged by size:
2774 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1977 2350 3128 3318 4178 1977
Size: 373 778 190 860 3135
Fragments arranged by size:
3135 860 778 373 190
Bsp1286I G_dGCh'C
Cuts at: 947 1034 1203 1977 2103 2350 2961 3054 3128
Size: 87 169 774 126 247 611 93 74
Cuts at: 3128 3318 3380 4178 947
Size: 190 62 798 2105
Fragments arranged by size:
2105 798 774 611 247 190 169 126
93 87 74 62
BspHI T'CATG_A
Cuts at: 3768 3768
Size: 5336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 674 2386 2600 2902 3283 674
Size: 1712 214 302 381 2727
Fragments arranged by size:
2727 1712 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1970 3628 3682 5166 5331 50
Size: 1920 1658 54 1484 165 55
Fragments arranged by size:
1920 1658 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3248 3248
Size: 5336
BsrFI r'CCGG_y
Cuts at: 3334 3515 3334
Size: 181 5155
BsrGI T'GTAC_A
Cuts at: 309 309
Size: 5336
BssSI C'ACGA_G
Cuts at: 3607 4315 3607
Size: 708 4628
BstAPI GCAn_nnn'nTGC
Cuts at: 198 1717 1822 1825 1885 2197 2713 198
Size: 1519 105 3 60 312 516 2821
Fragments arranged by size:
2821 1519 516 312 105 60 3
BstBI TT'CG_AA
Cuts at: 3697 3697
Size: 5336
BstXI CCAn_nnnn'nTGG
Cuts at: 177 271 177
Size: 94 5242
BstYI r'GATC_y
Cuts at: 1 125 590 624 2361 2828 2851 3186 3432
Size: 124 465 34 1737 467 23 335 246
Cuts at: 3432 3738 3750 3836 4888 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1737 1052 467 465 449 335 306 246
124 86 34 23 12
BtgI C'CryG_G
Cuts at: 1874 1874
Size: 5336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3286 3467 3630 3286
Size: 181 163 4992
Fragments arranged by size:
4992 181 163
BtsI GCAGTG_nn'
Cuts at: 515 1431 1518 2579 515
Size: 916 87 1061 3272
Fragments arranged by size:
3272 1061 916 87
DraI TTT'AAA
Cuts at: 305 2335 305
Size: 2030 3306
DrdI GACnn_nn'nnGTC
Cuts at: 3042 4386 3042
Size: 1344 3992
EaeI y'GGCC_r
Cuts at: 2921 3095 3486 3513 4858 2921
Size: 174 391 27 1345 3399
Fragments arranged by size:
3399 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2921 2921
Size: 5336
EarI CTCTTCn'nnn_
Cuts at: 1282 1316 2048 2275 3359 3569 1282
Size: 34 732 227 1084 210 3049
Fragments arranged by size:
3049 1084 732 227 210 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 513 552 3544 4286 4432 5146 513
Size: 39 2992 742 146 714 703
Fragments arranged by size:
2992 742 714 703 146 39
EcoICRI GAG'CTC
Cuts at: 2348 2348
Size: 5336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 437 454 569 1047 1173 1196 1577 1625
Size: 78 17 115 478 126 23 381 48
Cuts at: 1625 1952 3161 3593 3946 4608 359
Size: 327 1209 432 353 662 1087
Fragments arranged by size:
1209 1087 662 478 432 381 353 327
126 115 78 48 23 17
EcoNI CCTnn'n_nnAGG
Cuts at: 206 206
Size: 5336
EcoO109I rG'GnC_Cy
Cuts at: 1961 2475 1961
Size: 514 4822
EcoRI G'AATT_C
Cuts at: 1206 2340 1206
Size: 1134 4202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 377 1311 3355 377
Size: 934 2044 2358
Fragments arranged by size:
2358 2044 934
FspI TGC'GCA
Cuts at: 3117 3117
Size: 5336
HaeII r_GCGC'y
Cuts at: 1639 2770 3018 4248 5019 1639
Size: 1131 248 1230 771 1956
Fragments arranged by size:
1956 1230 1131 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 200 232 542 574 1326 1358 1588 1620 2106
Size: 32 310 32 752 32 230 32 486
Cuts at: 2106 2138 3178 3208 3210 3240 200
Size: 32 1040 30 2 30 2296
Fragments arranged by size:
2296 1040 752 486 310 230 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 908 2375 908
Size: 1467 3869
KasI G'GCGC_C
Cuts at: 2766 3014 2766
Size: 248 5088
KpnI G_GTAC'C
Cuts at: 2356 2356
Size: 5336
MluI A'CGCG_T
Cuts at: 4769 4769
Size: 5336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 288 715 1631 4095 4279 288
Size: 427 916 2464 184 1345
Fragments arranged by size:
2464 1345 916 427 184
MscI TGG'CCA
Cuts at: 3097 3097
Size: 5336
MslI CAynn'nnrTG
Cuts at: 580 1273 3325 3452 580
Size: 693 2052 127 2464
Fragments arranged by size:
2464 2052 693 127
MspA1I CmG'CkG
Cuts at: 495 1822 1882 2291 2447 2761 3121 3905 4150
Size: 1327 60 409 156 314 360 784 245
Cuts at: 4150 495
Size: 1681
Fragments arranged by size:
1681 1327 784 409 360 314 245 156
60
NaeI GCC'GGC
Cuts at: 3517 3517
Size: 5336
NarI GG'CG_CC
Cuts at: 2767 3015 2767
Size: 248 5088
NcoI C'CATG_G
Cuts at: 1874 1874
Size: 5336
NgoMIV G'CCGG_C
Cuts at: 3515 3515
Size: 5336
NheI G'CTAG_C
Cuts at: 411 411
Size: 5336
NspI r_CATG'y
Cuts at: 1119 2389 2889 4492 1119
Size: 1270 500 1603 1963
Fragments arranged by size:
1963 1603 1270 500
PasI CC'CwG_GG
Cuts at: 738 738
Size: 5336
PciI A'CATG_T
Cuts at: 1115 4488 1115
Size: 3373 1963
PflMI CCAn_nnn'nTGG
Cuts at: 1426 1788 1426
Size: 362 4974
PfoI T'CCnGG_A
Cuts at: 1212 1212
Size: 5336
PmeI GTTT'AAAC
Cuts at: 2335 2335
Size: 5336
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 5304
PpuMI rG'GwC_Cy
Cuts at: 1961 1961
Size: 5336
PstI C_TGCA'G
Cuts at: 510 1890 2202 2383 3068 510
Size: 1380 312 181 685 2778
Fragments arranged by size:
2778 1380 685 312 181
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5336
PvuII CAG'CTG
Cuts at: 495 1822 1882 2761 3121 495
Size: 1327 60 879 360 2710
Fragments arranged by size:
2710 1327 879 360 60
RsrII CG'GwC_CG
Cuts at: 3531 3531
Size: 5336
SacI G_AGCT'C
Cuts at: 2350 2350
Size: 5336
SalI G'TCGA_C
Cuts at: 2373 2373
Size: 5336
SanDI GG'GwC_CC
Cuts at: 1961 1961
Size: 5336
SapI GCTCTTCn'nnn_
Cuts at: 3359 3569 3359
Size: 210 5126
SbfI CC_TGCA'GG
Cuts at: 2383 2383
Size: 5336
ScaI AGT'ACT
Cuts at: 4853 4853
Size: 5336
SexAI A'CCwGG_T
Cuts at: 2075 2075
Size: 5336
SfcI C'TryA_G
Cuts at: 33 203 506 811 1886 2198 2216 2379 3064
Size: 170 303 305 1075 312 18 163 685
Cuts at: 3064 4032 4223 33
Size: 968 191 1146
Fragments arranged by size:
1146 1075 968 685 312 305 303 191
170 163 18
SfoI GGC'GCC
Cuts at: 2768 3016 2768
Size: 248 5088
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5336
SmaI CCC'GGG
Cuts at: 2358 2358
Size: 5336
SmlI C'TyrA_G
Cuts at: 955 964 1227 2491 3406 4120 4382 955
Size: 9 263 1264 915 714 262 1909
Fragments arranged by size:
1909 1264 915 714 263 262 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5336
SphI G_CATG'C
Cuts at: 2389 2889 2389
Size: 500 4836
StyI C'CwwG_G
Cuts at: 243 1874 2321 2470 2824 243
Size: 1631 447 149 354 2755
Fragments arranged by size:
2755 1631 447 354 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2919 3052 3511 3720 2919
Size: 133 459 209 4535
Fragments arranged by size:
4535 459 209 133
TatI w'GTAC_w
Cuts at: 309 2002 4851 309
Size: 1693 2849 794
Fragments arranged by size:
2849 1693 794
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1623 1655 1737 1769 2221 2253 4853 4885 1623
Size: 32 82 32 452 32 2600 32 2074
Fragments arranged by size:
2600 2074 452 82 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3133 3133
Size: 5336
XbaI T'CTAG_A
Cuts at: 2367 2367
Size: 5336
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1272 1785 2831 1272
Size: 513 1046 3777
Fragments arranged by size:
3777 1046 513
XmaI C'CCGG_G
Cuts at: 2356 2356
Size: 5336
XmnI GAAnn'nnTTC
Cuts at: 1291 1291
Size: 5336
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BsiWI BsmI
BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI