################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AccII AccIII AhdI Alw44I ApaLI AscI AspEI AspI AviII AvrII BalI BbeI BbrPI BcgI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiEI BsiWI Bsp1407I BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstUI CelII CpoI EagI Eam1105I EclXI Eco52I EcoNI FauI FseI FspI HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PvuI RcaI RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1817 GACGTC GACGT^C Acc65I 2257 GGTACC G^GTACC AclI 971 AACGTT AA^CGTT AflII 45 CTTAAG C^TTAAG AgeI 181 ACCGGT A^CCGGT AocI 539 CCTCAGG CC^T[ACGT]AGG Aor51HI 1382 AGCGCT AGC^GCT ApaI 1437 GGGCCC GGGCC^C Asp718I 2257 GGTACC G^GTACC BamHI 402 GGATCC G^GATCC BciVI 2995 GTATCC GTATCC(6/5) BclI 2268 TGATCA T^GATCA BfrI 45 CTTAAG C^TTAAG BglI 1635 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3045 AGATCT A^GATCT BsaWI 181 ACCGGT [AT]^CCGG[AT] BsmBI 24 CGTCTC CGTCTC(1/5) BspDI 2767 ATCGAT AT^CGAT BsrDI 155 CATTGC GCAATG(2/0) Bst1107I 2213 GTATAC GTA^TAC BstEII 211 GGTGACC G^GT[ACGT]ACC BstPI 211 GGTGACC G^GT[ACGT]ACC BstZ17I 2213 GTATAC GTA^TAC Bsu36I 539 CCTCAGG CC^T[ACGT]AGG ClaI 2767 ATCGAT AT^CGAT DraI 967 TTTAAA TTT^AAA DraIII 2687 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1281 TGGCCG [CT]^GGCC[GA] Eco47III 1382 AGCGCT AGC^GCT Eco81I 539 CCTCAGG CC^T[ACGT]AGG EcoO65I 211 GGTGACC G^GT[ACGT]ACC EcoRI 350 GAATTC G^AATTC EcoRV 1184 GATATC GAT^ATC FbaI 2268 TGATCA T^GATCA HaeII 1382 AGCGCT [GA]GCGC^[CT] HindIII 1586 AAGCTT A^AGCTT KpnI 2257 GGTACC GGTAC^C NcoI 2624 CCATGG C^CATGG NdeI 1514 CATATG CA^TATG NheI 2191 GCTAGC G^CTAGC PinAI 181 ACCGGT A^CCGGT PpuMI 307 AGGACCC [GA]G^G[AT]CC[CT] PshAI 3241 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 971 AACGTT AA^CGTT SacI 687 GAGCTC GAGCT^C SfiI 1634 GGCCAGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 2588 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccI 1943 GTCTAC GT^[AC][GT]AC 2213 GTATAC AcyI 1817 GACGTC G[GA]^CG[CT]C 2759 GGCGTC AseI 82 ATTAAT AT^TAAT 724 ATTAAT AsnI 82 ATTAAT AT^TAAT 724 ATTAAT AvaI 1657 CTCGGG C^[CT]CG[GA]G 3019 CCCGAG BanI 2257 GGTACC G^G[CT][GA]CC 2416 GGTGCC BanII 687 GAGCTC G[GA]GC[CT]^C 1437 GGGCCC BbvCI 1567 CCTCAGC CCTCAGC(-5/-2) 2869 CCTCAGC BcnI 1842 CCGGG CC^[GC]GG 2795 CCGGG BsaHI 1817 GACGTC G[GA]^CG[CT]C 2759 GGCGTC BsaI 184 GGTCTC GGTCTC(1/5) 573 GGTCTC BsgI 2330 GTGCAG GTGCAG(16/14) 2392 GTGCAG BsmI 1776 GAATGC GAATGC(1/-1) 1679 GCATTC BsoBI 1657 CTCGGG C^[CT]CG[GA]G 3019 CCCGAG BspLU11I 418 ACATGT A^CATGT 1736 ACATGT BsrFI 181 ACCGGT [GA]^CCGG[CT] 1865 GCCGGT BstXI 2201 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2999 CCATTGTACTGG Cfr10I 181 ACCGGT [GA]^CCGG[CT] 1865 GCCGGT DrdI 628 GACTTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3052 GACAGACTGGTC DsaI 2624 CCATGG C^C[GA][CT]GG 2646 CCGTGG EarI 2008 GAAGAG CTCTTC(1/4) 2291 GAAGAG EcoT14I 2624 CCATGG C^C[AT][AT]GG 2666 CCTTGG EcoT22I 339 ATGCAT ATGCA^T 1810 ATGCAT HgaI 369 GCGTC GACGC(5/10) 2760 GCGTC Hin1I 1817 GACGTC G[GA]^CG[CT]C 2759 GGCGTC HincII 150 GTCAAC GT[CT]^[GA]AC 2119 GTCAAC HindII 150 GTCAAC GT[CT]^[GA]AC 2119 GTCAAC Ksp632I 2008 GAAGAG CTCTTC(1/4) 2291 GAAGAG NciI 1842 CCGGG CC^[GC]GG 2795 CCGGG NsiI 339 ATGCAT ATGCA^T 1810 ATGCAT PflMI 312 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2619 CCATGCCATGG PshBI 82 ATTAAT AT^TAAT 724 ATTAAT PstI 1783 CTGCAG CTGCA^G 2722 CTGCAG SapI 2008 GAAGAGC GCTCTTC(1/4) 2291 GAAGAGC ScaI 1161 AGTACT AGT^ACT 1202 AGTACT SmlI 45 CTTAAG C^T[CT][GA]AG 978 CTTGAG SspI 1799 AATATT AAT^ATT 2944 AATATT StyI 2624 CCATGG C^C[AT][AT]GG 2666 CCTTGG Van91I 312 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2619 CCATGCCATGG XbaI 714 TCTAGA T^CTAGA 3048 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI