(Linear) (Six-base) MAPSORT of: KIBB9556.seq Check: 2995 from: 1 to: 2613 KIBB9556.orf With 182 enzymes: SgfI * March 3, 2008 11:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2421 2613 Size: 2421 192 AatII G_ACGT'C Cuts at: 0 2444 2613 Size: 2444 169 AccI GT'mk_AC Cuts at: 0 1610 2042 2613 Size: 1610 432 571 Fragments arranged by size: 1610 571 432 AflII C'TTAA_G Cuts at: 0 2121 2613 Size: 2121 492 AflIII A'CryG_T Cuts at: 0 2460 2545 2613 Size: 2460 85 68 Fragments arranged by size: 2460 85 68 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 626 658 1000 1032 2613 Size: 626 32 342 32 1581 Fragments arranged by size: 1581 626 342 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 87 2613 Size: 87 2526 ApaI G_GGCC'C Cuts at: 0 617 2613 Size: 617 1996 ApoI r'AATT_y Cuts at: 0 1398 1921 2613 Size: 1398 523 692 Fragments arranged by size: 1398 692 523 AvaI C'yCGr_G Cuts at: 0 472 682 2613 Size: 472 210 1931 Fragments arranged by size: 1931 472 210 AvrII C'CTAG_G Cuts at: 0 852 2613 Size: 852 1761 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1375 1408 2613 Size: 1375 33 1205 Fragments arranged by size: 1375 1205 33 BanI G'GyrC_C Cuts at: 0 201 253 357 507 1439 1867 2613 Size: 201 52 104 150 932 428 746 Fragments arranged by size: 932 746 428 201 150 104 52 BanII G_rGCy'C Cuts at: 0 127 241 605 617 680 812 1193 2218 Size: 127 114 364 12 63 132 381 1025 Cuts at: 2218 2313 2613 Size: 95 300 Fragments arranged by size: 1025 381 364 300 132 127 114 95 63 12 BbeI G_GCGC'C Cuts at: 0 257 1871 2613 Size: 257 1614 742 Fragments arranged by size: 1614 742 257 BbsI GAAGACnn'nnnn_ Cuts at: 0 1052 1697 2006 2613 Size: 1052 645 309 607 Fragments arranged by size: 1052 645 607 309 BbvCI CC'TCA_GC Cuts at: 0 71 2613 Size: 71 2542 BciVI GTATCCnnnnn_n' Cuts at: 0 1761 2613 Size: 1761 852 BglII A'GATC_T Cuts at: 0 1376 2613 Size: 1376 1237 BlpI GC'TnA_GC Cuts at: 0 2405 2613 Size: 2405 208 Bme1580I G_kGCm'C Cuts at: 0 206 617 2613 Size: 206 411 1996 Fragments arranged by size: 1996 411 206 BmgBI CAC'GTC Cuts at: 0 903 944 2613 Size: 903 41 1669 Fragments arranged by size: 1669 903 41 BmrI ACTGGGnnnn_n' Cuts at: 0 105 497 2613 Size: 105 392 2116 Fragments arranged by size: 2116 392 105 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 190 2613 Size: 190 2423 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2159 2613 Size: 2159 454 Bpu10I CC'TnA_GC Cuts at: 0 71 2613 Size: 71 2542 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 811 2001 2613 Size: 811 1190 612 Fragments arranged by size: 1190 811 612 BsaI GGTCTCn'nnnn_ Cuts at: 0 923 2596 2613 Size: 923 1673 17 Fragments arranged by size: 1673 923 17 BsaHI Gr'CG_yC Cuts at: 0 254 1868 2441 2613 Size: 254 1614 573 172 Fragments arranged by size: 1614 573 254 172 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 329 359 626 632 656 662 2613 Size: 329 30 267 6 24 6 1951 Fragments arranged by size: 1951 329 267 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 305 490 670 814 895 1941 2613 Size: 305 185 180 144 81 1046 672 Fragments arranged by size: 1046 672 305 185 180 144 81 BseYI C'CCAG_C Cuts at: 0 257 381 1019 1913 2613 Size: 257 124 638 894 700 Fragments arranged by size: 894 700 638 257 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 450 1581 2613 Size: 450 1131 1032 Fragments arranged by size: 1131 1032 450 BsiEI CG_ry'CG Cuts at: 0 64 576 2613 Size: 64 512 2037 Fragments arranged by size: 2037 512 64 BsiHKAI G_wGCw'C Cuts at: 0 680 2069 2218 2613 Size: 680 1389 149 395 Fragments arranged by size: 1389 680 395 149 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2287 2613 Size: 2287 326 Bsp1286I G_dGCh'C Cuts at: 0 127 206 241 605 617 680 812 1193 Size: 127 79 35 364 12 63 132 381 Cuts at: 1193 2069 2218 2313 2613 Size: 876 149 95 300 Fragments arranged by size: 876 381 364 300 149 132 127 95 79 63 35 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2421 2613 Size: 2421 192 BsrBI CCG'CTC Cuts at: 0 268 2613 Size: 268 2345 BsrDI GCAATG_nn' Cuts at: 0 1866 2613 Size: 1866 747 BsrFI r'CCGG_y Cuts at: 0 423 2613 Size: 423 2190 BsrGI T'GTAC_A Cuts at: 0 1898 2613 Size: 1898 715 BstEII G'GTnAC_C Cuts at: 0 396 1743 2613 Size: 396 1347 870 Fragments arranged by size: 1347 870 396 BstYI r'GATC_y Cuts at: 0 1243 1376 2613 Size: 1243 133 1237 Fragments arranged by size: 1243 1237 133 Bsu36I CC'TnA_GG Cuts at: 0 1790 2613 Size: 1790 823 BtgI C'CryG_G Cuts at: 0 439 695 2613 Size: 439 256 1918 Fragments arranged by size: 1918 439 256 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2483 2613 Size: 2483 130 BtsI GCAGTG_nn' Cuts at: 0 518 965 2613 Size: 518 447 1648 Fragments arranged by size: 1648 518 447 DraI TTT'AAA Cuts at: 0 1395 1926 2613 Size: 1395 531 687 Fragments arranged by size: 1395 687 531 DrdI GACnn_nn'nnGTC Cuts at: 0 866 2613 Size: 866 1747 EaeI y'GGCC_r Cuts at: 0 272 573 2613 Size: 272 301 2040 Fragments arranged by size: 2040 301 272 EagI C'GGCC_G Cuts at: 0 573 2613 Size: 573 2040 EarI CTCTTCn'nnn_ Cuts at: 0 751 1175 1959 2080 2153 2613 Size: 751 424 784 121 73 460 Fragments arranged by size: 784 751 460 424 121 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 69 2613 Size: 69 2544 EcoICRI GAG'CTC Cuts at: 0 678 2216 2613 Size: 678 1538 397 Fragments arranged by size: 1538 678 397 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2159 2613 Size: 2159 454 EcoNI CCTnn'n_nnAGG Cuts at: 0 557 2613 Size: 557 2056 EcoO109I rG'GnC_Cy Cuts at: 0 107 480 588 614 849 859 1158 2613 Size: 107 373 108 26 235 10 299 1455 Fragments arranged by size: 1455 373 299 235 108 107 26 10 FspI TGC'GCA Cuts at: 0 835 2613 Size: 835 1778 HaeII r_GCGC'y Cuts at: 0 257 1871 2613 Size: 257 1614 742 Fragments arranged by size: 1614 742 257 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1168 1200 1428 1460 2350 2382 2407 2439 Size: 1168 32 228 32 890 32 25 32 Cuts at: 2439 2532 2564 2613 Size: 93 32 49 Fragments arranged by size: 1168 890 228 93 49 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 0 494 2613 Size: 494 2119 KasI G'GCGC_C Cuts at: 0 253 1867 2613 Size: 253 1614 746 Fragments arranged by size: 1614 746 253 MfeI C'AATT_G Cuts at: 0 2558 2613 Size: 2558 55 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 815 1481 2613 Size: 815 666 1132 Fragments arranged by size: 1132 815 666 MscI TGG'CCA Cuts at: 0 274 2613 Size: 274 2339 MslI CAynn'nnrTG Cuts at: 0 2261 2613 Size: 2261 352 MspA1I CmG'CkG Cuts at: 0 261 441 1917 2315 2342 2613 Size: 261 180 1476 398 27 271 Fragments arranged by size: 1476 398 271 261 180 27 NarI GG'CG_CC Cuts at: 0 254 1868 2613 Size: 254 1614 745 Fragments arranged by size: 1614 745 254 NdeI CA'TA_TG Cuts at: 0 1584 2613 Size: 1584 1029 NspI r_CATG'y Cuts at: 0 2549 2613 Size: 2549 64 PasI CC'CwG_GG Cuts at: 0 864 913 2328 2613 Size: 864 49 1415 285 Fragments arranged by size: 1415 864 285 49 PciI A'CATG_T Cuts at: 0 2545 2613 Size: 2545 68 PflMI CCAn_nnn'nTGG Cuts at: 0 87 388 2613 Size: 87 301 2225 Fragments arranged by size: 2225 301 87 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 587 619 626 658 2613 Size: 587 32 7 32 1955 Fragments arranged by size: 1955 587 32 32 7 PpuMI rG'GwC_Cy Cuts at: 0 480 588 859 2613 Size: 480 108 271 1754 Fragments arranged by size: 1754 480 271 108 PspOMI G'GGCC_C Cuts at: 0 613 2613 Size: 613 2000 PstI C_TGCA'G Cuts at: 0 2358 2432 2613 Size: 2358 74 181 Fragments arranged by size: 2358 181 74 PvuII CAG'CTG Cuts at: 0 1917 2613 Size: 1917 696 SacI G_AGCT'C Cuts at: 0 680 2218 2613 Size: 680 1538 395 Fragments arranged by size: 1538 680 395 SacII CC_GC'GG Cuts at: 0 442 2613 Size: 442 2171 SapI GCTCTTCn'nnn_ Cuts at: 0 2153 2613 Size: 2153 460 SbfI CC_TGCA'GG Cuts at: 0 2432 2613 Size: 2432 181 SfcI C'TryA_G Cuts at: 0 1695 2354 2428 2613 Size: 1695 659 74 185 Fragments arranged by size: 1695 659 185 74 SfoI GGC'GCC Cuts at: 0 255 1869 2613 Size: 255 1614 744 Fragments arranged by size: 1614 744 255 SmlI C'TyrA_G Cuts at: 0 682 826 2016 2121 2613 Size: 682 144 1190 105 492 Fragments arranged by size: 1190 682 492 144 105 SspI AAT'ATT Cuts at: 0 1458 2613 Size: 1458 1155 StyI C'CwwG_G Cuts at: 0 852 2534 2613 Size: 852 1682 79 Fragments arranged by size: 1682 852 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 354 2375 2613 Size: 354 2021 238 Fragments arranged by size: 2021 354 238 TatI w'GTAC_w Cuts at: 0 299 706 1898 2613 Size: 299 407 1192 715 Fragments arranged by size: 1192 715 407 299 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 329 361 1552 1584 2613 Size: 329 32 1191 32 1029 Fragments arranged by size: 1191 1029 329 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2443 2613 Size: 2443 170 XhoI C'TCGA_G Cuts at: 0 682 2613 Size: 682 1931 ZraI GAC'GTC Cuts at: 0 2442 2613 Size: 2442 171 Enzymes that do cut: AarI AatII AccI AflII AflIII AloI AlwNI ApaI ApoI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BciVI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NarI NdeI NspI PasI PciI PflMI PpiI PpuMI PspOMI PstI PvuII SacI SacII SapI SbfI SfcI SfoI SmlI SspI StyI TaqII TatI TstI Tth111I XhoI ZraI Enzymes that do not cut: Acc65I AclI AcuI AfeI AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI BamHI BcgI BclI BfrBI BglI BmtI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BssHII BssSI BstAPI BstBI BstXI BstZ17I ClaI CspCI DraIII EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI XcmI XmaI XmnI