(Circular) (Six-base) MAPSORT of: pF1KB9555.seq Check: 5159 from: 1 to: 4451 pF1KB9555 4451 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 578 662 1294 578 Size: 84 632 3735 Fragments arranged by size: 3735 632 84 AccI GT'mk_AC Cuts at: 1489 1489 Size: 4451 Acc65I G'GTAC_C Cuts at: 1467 1467 Size: 4451 AclI AA'CG_TT Cuts at: 4291 4291 Size: 4451 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 199 537 621 1041 1125 2276 2708 3061 3723 Size: 338 84 420 84 1151 432 353 662 Cuts at: 3723 199 Size: 927 Fragments arranged by size: 1151 927 662 432 420 353 338 84 84 AflIII A'CryG_T Cuts at: 1364 1803 3603 3884 1364 Size: 439 1800 281 1931 Fragments arranged by size: 1931 1800 439 281 AgeI A'CCGG_T Cuts at: 1183 1313 1183 Size: 130 4321 AleI CACnn'nnGTG Cuts at: 1184 1184 Size: 4451 AlwNI CAG_nnn'CTG Cuts at: 1756 3194 1756 Size: 1438 3013 ApaLI G'TGCA_C Cuts at: 3289 3289 Size: 4451 ApoI r'AATT_y Cuts at: 303 839 1455 3804 4060 303 Size: 536 616 2349 256 694 Fragments arranged by size: 2349 694 616 536 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4451 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4451 AvaI C'yCGr_G Cuts at: 1471 1471 Size: 4451 BamHI G'GATC_C Cuts at: 1007 1476 1007 Size: 469 3982 BanI G'GyrC_C Cuts at: 662 1310 1467 1881 2129 2164 662 Size: 648 157 414 248 35 2949 Fragments arranged by size: 2949 648 414 248 157 35 BanII G_rGCy'C Cuts at: 377 443 565 649 733 817 901 1069 1153 Size: 66 122 84 84 84 84 168 84 Cuts at: 1153 1402 1465 2495 377 Size: 249 63 1030 2333 Fragments arranged by size: 2333 1030 249 168 122 84 84 84 84 84 66 63 BbeI G_GCGC'C Cuts at: 1885 2133 1885 Size: 248 4203 BbvCI CC'TCA_GC Cuts at: 409 409 Size: 4451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1393 1427 1393 Size: 34 4417 BciVI GTATCCnnnnn_n' Cuts at: 352 2349 3405 4451 352 Size: 1997 1056 1046 352 Fragments arranged by size: 1997 1056 1046 352 BclI T'GATC_A Cuts at: 1971 1971 Size: 4451 BglII A'GATC_T Cuts at: 1966 4003 1966 Size: 2037 2414 BlpI GC'TnA_GC Cuts at: 732 1563 732 Size: 831 3620 Bme1580I G_kGCm'C Cuts at: 2076 2169 3293 2076 Size: 93 1124 3234 Fragments arranged by size: 3234 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 437 605 1025 1663 1744 2079 4223 437 Size: 168 420 638 81 335 2144 665 Fragments arranged by size: 2144 665 638 420 335 168 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2818 2818 Size: 4451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 379 801 379 Size: 422 4029 Bpu10I CC'TnA_GC Cuts at: 409 658 900 907 409 Size: 249 242 7 3953 Fragments arranged by size: 3953 249 242 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 303 1627 2506 3220 3518 303 Size: 1324 879 714 298 1236 Fragments arranged by size: 1324 1236 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 133 849 4155 133 Size: 716 3306 429 Fragments arranged by size: 3306 716 429 BsaAI yAC'GTr Cuts at: 1365 1804 1365 Size: 439 4012 BsaBI GATnn'nnATC Cuts at: 6 1965 4002 6 Size: 1959 2037 455 Fragments arranged by size: 2037 1959 455 BsaHI Gr'CG_yC Cuts at: 1882 2130 2832 4328 1882 Size: 248 702 1496 2005 Fragments arranged by size: 2005 1496 702 248 BsaWI w'CCGG_w Cuts at: 763 1183 1313 1640 2161 2839 3250 3397 3671 Size: 420 130 327 521 678 411 147 274 Cuts at: 3671 763 Size: 1543 Fragments arranged by size: 1543 678 521 420 411 327 274 147 130 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1143 1143 Size: 4451 BseYI C'CCAG_C Cuts at: 120 1876 2634 3299 3707 120 Size: 1756 758 665 408 864 Fragments arranged by size: 1756 864 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 479 804 479 Size: 325 4126 BsiEI CG_ry'CG Cuts at: 104 1993 2039 3269 104 Size: 1889 46 1230 1286 Fragments arranged by size: 1889 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 319 377 565 649 733 817 901 1069 1085 Size: 58 188 84 84 84 84 168 16 Cuts at: 1085 1153 1169 1334 1402 1465 2243 2433 3293 Size: 68 16 165 68 63 778 190 860 Cuts at: 3293 319 Size: 1477 Fragments arranged by size: 1477 860 778 190 188 168 165 84 84 84 84 68 68 63 58 16 16 BsmI GAATG_Cn' Cuts at: 796 796 Size: 4451 Bsp1286I G_dGCh'C Cuts at: 319 377 443 565 649 733 817 901 1069 Size: 58 66 122 84 84 84 84 168 Cuts at: 1069 1085 1153 1169 1334 1402 1465 2076 2169 Size: 16 68 16 165 68 63 611 93 Cuts at: 2169 2243 2433 2495 3293 319 Size: 74 190 62 798 1477 Fragments arranged by size: 1477 798 611 190 168 165 122 93 84 84 84 84 74 68 68 66 63 62 58 16 16 BspHI T'CATG_A Cuts at: 2883 2883 Size: 4451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 578 662 1294 1501 1715 2017 2398 578 Size: 84 632 207 214 302 381 2631 Fragments arranged by size: 2631 632 381 302 214 207 84 BsrBI CCG'CTC Cuts at: 50 2743 2797 4281 4446 50 Size: 2693 54 1484 165 55 Fragments arranged by size: 2693 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2363 2363 Size: 4451 BsrFI r'CCGG_y Cuts at: 1183 1267 1313 2449 2630 1183 Size: 84 46 1136 181 3004 Fragments arranged by size: 3004 1136 181 84 46 BssSI C'ACGA_G Cuts at: 832 946 2722 3430 832 Size: 114 1776 708 1853 Fragments arranged by size: 1853 1776 708 114 BstAPI GCAn_nnn'nTGC Cuts at: 791 1376 1828 791 Size: 585 452 3414 Fragments arranged by size: 3414 585 452 BstBI TT'CG_AA Cuts at: 2812 2812 Size: 4451 BstXI CCAn_nnnn'nTGG Cuts at: 1129 1129 Size: 4451 BstYI r'GATC_y Cuts at: 1 257 755 923 1007 1476 1943 1966 2301 Size: 256 498 168 84 469 467 23 335 Cuts at: 2301 2547 2853 2865 2951 4003 1 Size: 246 306 12 86 1052 449 Fragments arranged by size: 1052 498 469 467 449 335 306 256 246 168 86 84 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4451 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1052 2401 2582 2745 1052 Size: 1349 181 163 2758 Fragments arranged by size: 2758 1349 181 163 BtsI GCAGTG_nn' Cuts at: 1694 1694 Size: 4451 DraI TTT'AAA Cuts at: 1450 1450 Size: 4451 DraIII CAC_nnn'GTG Cuts at: 585 585 Size: 4451 DrdI GACnn_nn'nnGTC Cuts at: 2157 3501 2157 Size: 1344 3107 EaeI y'GGCC_r Cuts at: 2036 2210 2601 2628 3973 2036 Size: 174 391 27 1345 2514 Fragments arranged by size: 2514 1345 391 174 27 EagI C'GGCC_G Cuts at: 2036 2036 Size: 4451 EarI CTCTTCn'nnn_ Cuts at: 382 431 2474 2684 382 Size: 49 2043 210 2149 Fragments arranged by size: 2149 2043 210 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 712 2659 3401 3547 4261 712 Size: 1947 742 146 714 902 Fragments arranged by size: 1947 902 742 714 146 EcoICRI GAG'CTC Cuts at: 375 563 647 731 815 899 1067 1151 1400 Size: 188 84 84 84 84 168 84 249 Cuts at: 1400 1463 375 Size: 63 3363 Fragments arranged by size: 3363 249 188 168 84 84 84 84 84 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 199 379 537 621 801 1041 1125 2276 2708 Size: 180 158 84 180 240 84 1151 432 Cuts at: 2708 3061 3723 199 Size: 353 662 927 Fragments arranged by size: 1151 927 662 432 353 240 180 180 158 84 84 EcoO109I rG'GnC_Cy Cuts at: 195 204 484 773 1590 195 Size: 9 280 289 817 3056 Fragments arranged by size: 3056 817 289 280 9 EcoRI G'AATT_C Cuts at: 839 1455 839 Size: 616 3835 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2470 2470 Size: 4451 FspI TGC'GCA Cuts at: 2232 2232 Size: 4451 HaeII r_GCGC'y Cuts at: 1885 2133 3363 4134 1885 Size: 248 1230 771 2202 Fragments arranged by size: 2202 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 846 878 900 932 1401 1433 2293 2323 2325 Size: 32 22 32 469 32 860 30 2 Cuts at: 2325 2355 846 Size: 30 2942 Fragments arranged by size: 2942 860 469 32 32 32 30 30 22 2 HincII GTy'rAC Cuts at: 1490 1490 Size: 4451 KasI G'GCGC_C Cuts at: 1881 2129 1881 Size: 248 4203 KpnI G_GTAC'C Cuts at: 1471 1471 Size: 4451 MluI A'CGCG_T Cuts at: 3884 3884 Size: 4451 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 428 600 1020 1094 3210 3394 428 Size: 172 420 74 2116 184 1485 Fragments arranged by size: 2116 1485 420 184 172 74 MscI TGG'CCA Cuts at: 2212 2212 Size: 4451 MslI CAynn'nnrTG Cuts at: 1032 1184 2440 2567 1032 Size: 152 1256 127 2916 Fragments arranged by size: 2916 1256 152 127 MspA1I CmG'CkG Cuts at: 1005 1562 1876 2236 3020 3265 1005 Size: 557 314 360 784 245 2191 Fragments arranged by size: 2191 784 557 360 314 245 NaeI GCC'GGC Cuts at: 2632 2632 Size: 4451 NarI GG'CG_CC Cuts at: 1882 2130 1882 Size: 248 4203 NcoI C'CATG_G Cuts at: 107 107 Size: 4451 NgoMIV G'CCGG_C Cuts at: 2630 2630 Size: 4451 NspI r_CATG'y Cuts at: 417 1504 2004 3607 417 Size: 1087 500 1603 1261 Fragments arranged by size: 1603 1261 1087 500 PasI CC'CwG_GG Cuts at: 187 187 Size: 4451 PciI A'CATG_T Cuts at: 3603 3603 Size: 4451 PmeI GTTT'AAAC Cuts at: 1450 1450 Size: 4451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2897 2929 2897 Size: 32 4419 PpuMI rG'GwC_Cy Cuts at: 195 204 484 195 Size: 9 280 4162 Fragments arranged by size: 4162 280 9 PstI C_TGCA'G Cuts at: 1498 2183 1498 Size: 685 3766 PvuI CG_AT'CG Cuts at: 104 104 Size: 4451 PvuII CAG'CTG Cuts at: 1876 2236 1876 Size: 360 4091 RsrII CG'GwC_CG Cuts at: 2646 2646 Size: 4451 SacI G_AGCT'C Cuts at: 377 565 649 733 817 901 1069 1153 1402 Size: 188 84 84 84 84 168 84 249 Cuts at: 1402 1465 377 Size: 63 3363 Fragments arranged by size: 3363 249 188 168 84 84 84 84 84 63 SalI G'TCGA_C Cuts at: 1488 1488 Size: 4451 SanDI GG'GwC_CC Cuts at: 204 204 Size: 4451 SapI GCTCTTCn'nnn_ Cuts at: 382 431 2474 2684 382 Size: 49 2043 210 2149 Fragments arranged by size: 2149 2043 210 49 SbfI CC_TGCA'GG Cuts at: 1498 1498 Size: 4451 ScaI AGT'ACT Cuts at: 3968 3968 Size: 4451 SexAI A'CCwGG_T Cuts at: 581 581 Size: 4451 SfcI C'TryA_G Cuts at: 33 1494 2179 3147 3338 33 Size: 1461 685 968 191 1146 Fragments arranged by size: 1461 1146 968 685 191 SfoI GGC'GCC Cuts at: 1883 2131 1883 Size: 248 4203 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4451 SgrAI Cr'CCGG_yG Cuts at: 1183 1183 Size: 4451 SmaI CCC'GGG Cuts at: 1473 1473 Size: 4451 SmlI C'TyrA_G Cuts at: 318 1606 2521 3235 3497 318 Size: 1288 915 714 262 1272 Fragments arranged by size: 1288 1272 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4451 SphI G_CATG'C Cuts at: 1504 2004 1504 Size: 500 3951 StuI AGG'CCT Cuts at: 550 634 718 905 1054 1222 550 Size: 84 84 187 149 168 3779 Fragments arranged by size: 3779 187 168 149 84 84 StyI C'CwwG_G Cuts at: 107 1585 1939 107 Size: 1478 354 2619 Fragments arranged by size: 2619 1478 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2034 2167 2626 2835 2034 Size: 133 459 209 3650 Fragments arranged by size: 3650 459 209 133 TatI w'GTAC_w Cuts at: 3966 3966 Size: 4451 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 977 1009 3968 4000 977 Size: 32 2959 32 1428 Fragments arranged by size: 2959 1428 32 32 Tth111I GACn'n_nGTC Cuts at: 2248 2248 Size: 4451 XbaI T'CTAG_A Cuts at: 1482 1482 Size: 4451 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1946 1946 Size: 4451 XmaI C'CCGG_G Cuts at: 1471 1471 Size: 4451 XmnI GAAnn'nnTTC Cuts at: 550 634 718 802 886 1054 1138 550 Size: 84 84 84 84 168 84 3863 Fragments arranged by size: 3863 168 84 84 84 84 84 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI SnaBI SrfI SspI SwaI XhoI ZraI