(Circular) (Six-base) MAPSORT of: pF1KB9551.seq Check: 5031 from: 1 to: 4268 pF1KB9551 4268 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AccI GT'mk_AC Cuts at: 1306 1306 Size: 4268 Acc65I G'GTAC_C Cuts at: 501 1284 501 Size: 783 3485 AclI AA'CG_TT Cuts at: 4108 4108 Size: 4268 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1012 1074 2093 2525 2878 3540 1012 Size: 62 1019 432 353 662 1740 Fragments arranged by size: 1740 1019 662 432 353 62 AflIII A'CryG_T Cuts at: 953 1620 3420 3701 953 Size: 667 1800 281 1520 Fragments arranged by size: 1800 1520 667 281 AleI CACnn'nnGTG Cuts at: 367 367 Size: 4268 AlwNI CAG_nnn'CTG Cuts at: 705 1183 1573 3011 705 Size: 478 390 1438 1962 Fragments arranged by size: 1962 1438 478 390 ApaLI G'TGCA_C Cuts at: 3106 3106 Size: 4268 ApoI r'AATT_y Cuts at: 1272 3621 3877 1272 Size: 2349 256 1663 Fragments arranged by size: 2349 1663 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4268 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4268 AvaI C'yCGr_G Cuts at: 655 1288 655 Size: 633 3635 BamHI G'GATC_C Cuts at: 1293 1293 Size: 4268 BanI G'GyrC_C Cuts at: 501 1154 1284 1698 1946 1981 501 Size: 653 130 414 248 35 2788 Fragments arranged by size: 2788 653 414 248 130 35 BanII G_rGCy'C Cuts at: 455 1282 2312 455 Size: 827 1030 2411 Fragments arranged by size: 2411 1030 827 BbeI G_GCGC'C Cuts at: 1702 1950 1702 Size: 248 4020 BbsI GAAGACnn'nnnn_ Cuts at: 1084 1084 Size: 4268 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 117 151 117 Size: 34 4234 BciVI GTATCCnnnnn_n' Cuts at: 340 2166 3222 4268 340 Size: 1826 1056 1046 340 Fragments arranged by size: 1826 1056 1046 340 BclI T'GATC_A Cuts at: 1788 1788 Size: 4268 BglI GCCn_nnn'nGGC Cuts at: 1249 1249 Size: 4268 BglII A'GATC_T Cuts at: 1783 3820 1783 Size: 2037 2231 BlpI GC'TnA_GC Cuts at: 124 1063 1380 124 Size: 939 317 3012 Fragments arranged by size: 3012 939 317 Bme1580I G_kGCm'C Cuts at: 1159 1893 1986 3110 1159 Size: 734 93 1124 2317 Fragments arranged by size: 2317 1124 734 93 BmrI ACTGGGnnnn_n' Cuts at: 1480 1561 1896 4040 1480 Size: 81 335 2144 1708 Fragments arranged by size: 2144 1708 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2635 2635 Size: 4268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 184 279 889 1186 184 Size: 95 610 297 3266 Fragments arranged by size: 3266 610 297 95 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1444 2323 3037 3335 1444 Size: 879 714 298 2377 Fragments arranged by size: 2377 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 3972 3972 Size: 4268 BsaAI yAC'GTr Cuts at: 1621 1621 Size: 4268 BsaBI GATnn'nnATC Cuts at: 6 1123 1782 3819 6 Size: 1117 659 2037 455 Fragments arranged by size: 2037 1117 659 455 BsaHI Gr'CG_yC Cuts at: 713 748 1236 1699 1947 2649 4145 713 Size: 35 488 463 248 702 1496 836 Fragments arranged by size: 1496 836 702 488 463 248 35 BsaWI w'CCGG_w Cuts at: 1127 1457 1978 2656 3067 3214 3488 1127 Size: 330 521 678 411 147 274 1907 Fragments arranged by size: 1907 678 521 411 330 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 862 892 997 1027 862 Size: 30 105 30 4103 Fragments arranged by size: 4103 105 30 30 BseYI C'CCAG_C Cuts at: 624 1008 1693 2451 3116 3524 624 Size: 384 685 758 665 408 1368 Fragments arranged by size: 1368 758 685 665 408 384 BsiEI CG_ry'CG Cuts at: 104 1810 1856 3086 104 Size: 1706 46 1230 1286 Fragments arranged by size: 1706 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1003 1282 2060 2250 3110 1003 Size: 279 778 190 860 2161 Fragments arranged by size: 2161 860 778 279 190 BsmBI CGTCTCn'nnnn_ Cuts at: 704 704 Size: 4268 Bsp1286I G_dGCh'C Cuts at: 455 1003 1159 1282 1893 1986 2060 2250 2312 Size: 548 156 123 611 93 74 190 62 Cuts at: 2312 3110 455 Size: 798 1613 Fragments arranged by size: 1613 798 611 548 190 156 123 93 74 62 BspEI T'CCGG_A Cuts at: 1127 1127 Size: 4268 BspHI T'CATG_A Cuts at: 2700 2700 Size: 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1318 1532 1834 2215 1318 Size: 214 302 381 3371 Fragments arranged by size: 3371 381 302 214 BsrBI CCG'CTC Cuts at: 50 2560 2614 4098 4263 50 Size: 2510 54 1484 165 55 Fragments arranged by size: 2510 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2180 2180 Size: 4268 BsrFI r'CCGG_y Cuts at: 2266 2447 2266 Size: 181 4087 BsrGI T'GTAC_A Cuts at: 236 236 Size: 4268 BssSI C'ACGA_G Cuts at: 1221 2539 3247 1221 Size: 1318 708 2242 Fragments arranged by size: 2242 1318 708 BstAPI GCAn_nnn'nTGC Cuts at: 705 1645 705 Size: 940 3328 BstBI TT'CG_AA Cuts at: 2629 2629 Size: 4268 BstXI CCAn_nnnn'nTGG Cuts at: 678 678 Size: 4268 BstYI r'GATC_y Cuts at: 1 1199 1293 1760 1783 2118 2364 2670 2682 Size: 1198 94 467 23 335 246 306 12 Cuts at: 2682 2768 3820 1 Size: 86 1052 449 Fragments arranged by size: 1198 1052 467 449 335 306 246 94 86 23 12 Bsu36I CC'TnA_GG Cuts at: 466 466 Size: 4268 BtgI C'CryG_G Cuts at: 854 854 Size: 4268 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 801 1023 1154 2218 2399 2562 801 Size: 222 131 1064 181 163 2507 Fragments arranged by size: 2507 1064 222 181 163 131 BtsI GCAGTG_nn' Cuts at: 1159 1511 1159 Size: 352 3916 ClaI AT'CG_AT Cuts at: 692 692 Size: 4268 DraI TTT'AAA Cuts at: 1267 1267 Size: 4268 DrdI GACnn_nn'nnGTC Cuts at: 889 1974 3318 889 Size: 1085 1344 1839 Fragments arranged by size: 1839 1344 1085 EaeI y'GGCC_r Cuts at: 1853 2027 2418 2445 3790 1853 Size: 174 391 27 1345 2331 Fragments arranged by size: 2331 1345 391 174 27 EagI C'GGCC_G Cuts at: 1853 1853 Size: 4268 EarI CTCTTCn'nnn_ Cuts at: 275 392 583 2291 2501 275 Size: 117 191 1708 210 2042 Fragments arranged by size: 2042 1708 210 191 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1242 2476 3218 3364 4078 1242 Size: 1234 742 146 714 1432 Fragments arranged by size: 1432 1234 742 714 146 EcoICRI GAG'CTC Cuts at: 1280 1280 Size: 4268 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 184 279 889 1012 1074 1186 2093 2525 2878 Size: 95 610 123 62 112 907 432 353 Cuts at: 2878 3540 184 Size: 662 912 Fragments arranged by size: 912 907 662 610 432 353 123 112 95 62 EcoNI CCTnn'n_nnAGG Cuts at: 465 465 Size: 4268 EcoO109I rG'GnC_Cy Cuts at: 1407 1407 Size: 4268 EcoRI G'AATT_C Cuts at: 1272 1272 Size: 4268 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 612 2287 612 Size: 1675 2593 FspI TGC'GCA Cuts at: 2049 2049 Size: 4268 HaeII r_GCGC'y Cuts at: 124 1702 1950 3180 3951 124 Size: 1578 248 1230 771 441 Fragments arranged by size: 1578 1230 771 441 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 631 663 824 856 862 894 1056 1088 1221 Size: 32 161 32 6 32 162 32 133 Cuts at: 1221 1253 2110 2140 2142 2172 631 Size: 32 857 30 2 30 2727 Fragments arranged by size: 2727 857 162 161 133 32 32 32 32 32 30 30 6 2 HincII GTy'rAC Cuts at: 1307 1307 Size: 4268 KasI G'GCGC_C Cuts at: 1698 1946 1698 Size: 248 4020 KpnI G_GTAC'C Cuts at: 505 1288 505 Size: 783 3485 MluI A'CGCG_T Cuts at: 3701 3701 Size: 4268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3027 3211 3027 Size: 184 4084 MscI TGG'CCA Cuts at: 2029 2029 Size: 4268 MslI CAynn'nnrTG Cuts at: 112 367 533 913 2257 2384 112 Size: 255 166 380 1344 127 1996 Fragments arranged by size: 1996 1344 380 255 166 127 MspA1I CmG'CkG Cuts at: 162 628 798 1062 1379 1693 2053 2837 3082 Size: 466 170 264 317 314 360 784 245 Cuts at: 3082 162 Size: 1348 Fragments arranged by size: 1348 784 466 360 317 314 264 245 170 NaeI GCC'GGC Cuts at: 2449 2449 Size: 4268 NarI GG'CG_CC Cuts at: 1699 1947 1699 Size: 248 4020 NgoMIV G'CCGG_C Cuts at: 2447 2447 Size: 4268 NspI r_CATG'y Cuts at: 957 1321 1821 3424 957 Size: 364 500 1603 1801 Fragments arranged by size: 1801 1603 500 364 PciI A'CATG_T Cuts at: 953 3420 953 Size: 2467 1801 PfoI T'CCnGG_A Cuts at: 866 866 Size: 4268 PmeI GTTT'AAAC Cuts at: 1267 1267 Size: 4268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2714 2746 2714 Size: 32 4236 PstI C_TGCA'G Cuts at: 1315 2000 1315 Size: 685 3583 PvuI CG_AT'CG Cuts at: 104 104 Size: 4268 PvuII CAG'CTG Cuts at: 162 628 1062 1693 2053 162 Size: 466 434 631 360 2377 Fragments arranged by size: 2377 631 466 434 360 RsrII CG'GwC_CG Cuts at: 2463 2463 Size: 4268 SacI G_AGCT'C Cuts at: 1282 1282 Size: 4268 SalI G'TCGA_C Cuts at: 1305 1305 Size: 4268 SapI GCTCTTCn'nnn_ Cuts at: 2291 2501 2291 Size: 210 4058 SbfI CC_TGCA'GG Cuts at: 1315 1315 Size: 4268 ScaI AGT'ACT Cuts at: 3785 3785 Size: 4268 SfcI C'TryA_G Cuts at: 33 1311 1996 2964 3155 33 Size: 1278 685 968 191 1146 Fragments arranged by size: 1278 1146 968 685 191 SfoI GGC'GCC Cuts at: 1700 1948 1700 Size: 248 4020 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4268 SmaI CCC'GGG Cuts at: 1290 1290 Size: 4268 SmlI C'TyrA_G Cuts at: 1423 2338 3052 3314 1423 Size: 915 714 262 2377 Fragments arranged by size: 2377 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4268 SphI G_CATG'C Cuts at: 1321 1821 1321 Size: 500 3768 StyI C'CwwG_G Cuts at: 1402 1756 1402 Size: 354 3914 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1851 1984 2443 2652 1851 Size: 133 459 209 3467 Fragments arranged by size: 3467 459 209 133 TatI w'GTAC_w Cuts at: 236 425 3783 236 Size: 189 3358 721 Fragments arranged by size: 3358 721 189 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1138 1170 3785 3817 1138 Size: 32 2615 32 1589 Fragments arranged by size: 2615 1589 32 32 Tth111I GACn'n_nGTC Cuts at: 2065 2065 Size: 4268 XbaI T'CTAG_A Cuts at: 1299 1299 Size: 4268 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 1763 114 Size: 1649 2619 XmaI C'CCGG_G Cuts at: 1288 1288 Size: 4268 XmnI GAAnn'nnTTC Cuts at: 401 924 401 Size: 523 3745 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmI BssHII BstEII BstZ17I CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI