################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI Fnu4HI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeII MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I TseI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 190 CGCG CG^CG AfaI 177 GTAC GT^AC Aor51HI 207 AGCGCT AGC^GCT AspHI 364 GAGCTC G[AT]GC[AT]^C AvaI 203 CCCGAG C^[CT]CG[GA]G BanI 199 GGCACC G^G[CT][GA]CC BbsI 346 GAAGAC GAAGAC(2/6) BfaI 64 CTAG C^TAG BpuAI 346 GAAGAC GAAGAC(2/6) BsaBI 303 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 49 GAGGAG GAGGAG(10/8) BsiEI 130 CGACCG CG[GA][CT]^CG BsiHKAI 364 GAGCTC G[AT]GC[AT]^C BsmAI 256 GAGAC GTCTC(1/5) BsmI 78 GAATGC GAATGC(1/-1) BsoBI 203 CCCGAG C^[CT]CG[GA]G BspMI 326 ACCTGC ACCTGC(4/8) BsrDI 273 CATTGC GCAATG(2/0) BsrI 350 ACTGG ACTGG(1/-1) BssKI 96 CCAGG ^CC[ACGT]GG BstF5I 54 GGATG GGATG(2/0) BstNI 96 CCAGG CC^[AT]GG BstUI 190 CGCG CG^CG CfoI 208 GCGC GCG^C Cfr13I 294 GGCCC G^G[ACGT]CC EarI 85 GAAGAG CTCTTC(1/4) Eco47III 207 AGCGCT AGC^GCT EcoRII 96 CCAGG ^CC[AT]GG FauI 188 CCCGC CCCGC(4/6) FokI 54 GGATG GGATG(9/13) HaeII 207 AGCGCT [GA]GCGC^[CT] HhaI 208 GCGC GCG^C HinP1I 208 GCGC G^CGC HinfI 152 GAATC G^A[ACGT]TC Ksp632I 85 GAAGAG CTCTTC(1/4) MaeI 64 CTAG C^TAG MaeIII 323 GTTAC ^GT[ACGT]AC MamI 303 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 273 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 96 CCAGG CC^[AT]GG MvnI 190 CGCG CG^CG NlaIV 199 GGCACC GG[ACGT]^[ACGT]CC RsaI 177 GTAC GT^AC SacI 364 GAGCTC GAGCT^C Sau96I 294 GGCCC G^G[ACGT]CC ScrFI 96 CCAGG CC^[ACGT]GG SmlI 289 CTCAAG C^T[CT][GA]AG TaqI 107 TCGA T^CGA TfiI 152 GAATC G^A[AT]TC Tsp509I 19 AATT ^AATT TthHB8I 107 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AciI 133 CCGC CCGC(-3/-1) 189 CCGC BanII 185 GAGCCC G[GA]GC[CT]^C 364 GAGCTC BpmI 211 CTCCAG CTGGAG(16/14) 247 CTGGAG HphI 155 TCACC GGTGA(8/7) 374 TCACC MboII 85 GAAGA GAAGA(8/7) 346 GAAGA MwoI 191 GCGTGCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 220 GCGATGTGTGC NlaIII 168 CATG CATG^ 279 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AluI AlwI BmyI BsiYI BslI Bsp1286I BstYI Cac8I DpnII HaeIII MboI MflI MnlI NdeII Sau3AI XhoII