(Circular) (Six-base) MAPSORT of: pF1KB9521.seq Check: 7560 from: 1 to: 4877 pF1KB9521 4877 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 427 427 Size: 4877 AccI GT'mk_AC Cuts at: 1915 1915 Size: 4877 Acc65I G'GTAC_C Cuts at: 808 828 1893 808 Size: 20 1065 3792 Fragments arranged by size: 3792 1065 20 AclI AA'CG_TT Cuts at: 4717 4717 Size: 4877 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 639 1541 2702 3134 3881 4149 639 Size: 902 1161 432 747 268 1367 Fragments arranged by size: 1367 1161 902 747 432 268 AfeI AGC'GCT Cuts at: 592 1093 592 Size: 501 4376 AflIII A'CryG_T Cuts at: 2229 3333 4310 2229 Size: 1104 977 2796 Fragments arranged by size: 2796 1104 977 AgeI A'CCGG_T Cuts at: 3265 3265 Size: 4877 AleI CACnn'nnGTG Cuts at: 961 961 Size: 4877 AlwNI CAG_nnn'CTG Cuts at: 510 723 1059 2182 3749 510 Size: 213 336 1123 1567 1638 Fragments arranged by size: 1638 1567 1123 336 213 ApaI G_GGCC'C Cuts at: 732 1452 1840 732 Size: 720 388 3769 Fragments arranged by size: 3769 720 388 ApaLI G'TGCA_C Cuts at: 3647 3647 Size: 4877 ApoI r'AATT_y Cuts at: 1881 4230 4486 1881 Size: 2349 256 2272 Fragments arranged by size: 2349 2272 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4877 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4877 AvaI C'yCGr_G Cuts at: 113 854 1376 1453 1897 113 Size: 741 522 77 444 3093 Fragments arranged by size: 3093 741 522 444 77 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 354 387 354 Size: 33 4844 BamHI G'GATC_C Cuts at: 1828 1902 1828 Size: 74 4803 BanI G'GyrC_C Cuts at: 808 828 963 1008 1893 2307 2555 2590 808 Size: 20 135 45 885 414 248 35 3095 Fragments arranged by size: 3095 885 414 248 135 45 35 20 BanII G_rGCy'C Cuts at: 182 732 747 1452 1840 1891 2921 182 Size: 550 15 705 388 51 1030 2138 Fragments arranged by size: 2138 1030 705 550 388 51 15 BbeI G_GCGC'C Cuts at: 1012 2311 2559 1012 Size: 1299 248 3330 Fragments arranged by size: 3330 1299 248 BbsI GAAGACnn'nnnn_ Cuts at: 375 375 Size: 4877 BbvCI CC'TCA_GC Cuts at: 214 487 214 Size: 273 4604 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 316 350 366 400 1663 1697 316 Size: 34 16 34 1263 34 3496 Fragments arranged by size: 3496 1263 34 34 34 16 BciVI GTATCCnnnnn_n' Cuts at: 2775 3536 4877 2775 Size: 761 1341 2775 Fragments arranged by size: 2775 1341 761 BclI T'GATC_A Cuts at: 2397 2397 Size: 4877 BglI GCCn_nnn'nGGC Cuts at: 1278 1278 Size: 4877 BglII A'GATC_T Cuts at: 2392 4429 2392 Size: 2037 2840 BlpI GC'TnA_GC Cuts at: 1989 1989 Size: 4877 Bme1580I G_kGCm'C Cuts at: 280 305 732 968 1452 1676 1840 2502 2595 Size: 25 427 236 484 224 164 662 93 Cuts at: 2595 3651 280 Size: 1056 1506 Fragments arranged by size: 1506 1056 662 484 427 236 224 164 93 25 BmgBI CAC'GTC Cuts at: 1107 1107 Size: 4877 BmrI ACTGGGnnnn_n' Cuts at: 1708 2089 2170 2505 4649 1708 Size: 381 81 335 2144 1936 Fragments arranged by size: 2144 1936 381 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 156 3244 156 Size: 3088 1789 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 271 271 Size: 4877 Bpu10I CC'TnA_GC Cuts at: 214 487 214 Size: 273 4604 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 979 1321 2053 2932 3424 3722 979 Size: 342 732 879 492 298 2134 Fragments arranged by size: 2134 879 732 492 342 298 BsaI GGTCTCn'nnnn_ Cuts at: 4581 4581 Size: 4877 BsaAI yAC'GTr Cuts at: 2230 2230 Size: 4877 BsaBI GATnn'nnATC Cuts at: 6 2391 4428 6 Size: 2385 2037 455 Fragments arranged by size: 2385 2037 455 BsaHI Gr'CG_yC Cuts at: 1009 1338 2308 2556 3258 4754 1009 Size: 329 970 248 702 1496 1132 Fragments arranged by size: 1496 1132 970 702 329 248 BsaWI w'CCGG_w Cuts at: 2066 2587 3265 3539 3686 4097 2066 Size: 521 678 274 147 411 2846 Fragments arranged by size: 2846 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 148 1162 1286 1516 1630 1670 148 Size: 1014 124 230 114 40 3355 Fragments arranged by size: 3355 1014 230 124 114 40 BseYI C'CCAG_C Cuts at: 1434 1859 2302 3060 3637 4133 1434 Size: 425 443 758 577 496 2178 Fragments arranged by size: 2178 758 577 496 443 425 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 346 1381 346 Size: 1035 3842 BsiEI CG_ry'CG Cuts at: 104 431 2419 2465 3673 104 Size: 327 1988 46 1208 1308 Fragments arranged by size: 1988 1308 1208 327 46 BsiHKAI G_wGCw'C Cuts at: 1045 1891 2669 2859 3651 1045 Size: 846 778 190 792 2271 Fragments arranged by size: 2271 846 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 1773 1773 Size: 4877 Bsp1286I G_dGCh'C Cuts at: 182 280 305 732 747 968 1045 1452 1676 Size: 98 25 427 15 221 77 407 224 Cuts at: 1676 1840 1891 2502 2595 2669 2859 2921 3651 Size: 164 51 611 93 74 190 62 730 Cuts at: 3651 182 Size: 1408 Fragments arranged by size: 1408 730 611 427 407 224 221 190 164 98 93 77 74 62 51 25 15 BspEI T'CCGG_A Cuts at: 4097 4097 Size: 4877 BspHI T'CATG_A Cuts at: 4053 4053 Size: 4877 BspMI ACCTGCnnnn'nnnn_ Cuts at: 427 900 1927 2141 2443 2824 427 Size: 473 1027 214 302 381 2480 Fragments arranged by size: 2480 1027 473 381 302 214 BsrBI CCG'CTC Cuts at: 50 3169 3223 4707 4872 50 Size: 3119 54 1484 165 55 Fragments arranged by size: 3119 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1132 2789 1132 Size: 1657 3220 BsrFI r'CCGG_y Cuts at: 2875 3056 3265 2875 Size: 181 209 4487 Fragments arranged by size: 4487 209 181 BssSI C'ACGA_G Cuts at: 3148 3506 3148 Size: 358 4519 BstAPI GCAn_nnn'nTGC Cuts at: 510 514 2254 510 Size: 4 1740 3133 Fragments arranged by size: 3133 1740 4 BstBI TT'CG_AA Cuts at: 3238 3238 Size: 4877 BstEII G'GTnAC_C Cuts at: 912 912 Size: 4877 BstXI CCAn_nnnn'nTGG Cuts at: 843 975 843 Size: 132 4745 BstYI r'GATC_y Cuts at: 1 1828 1902 2369 2392 2727 2973 3985 4071 Size: 1827 74 467 23 335 246 1012 86 Cuts at: 4071 4083 4429 1 Size: 12 346 449 Fragments arranged by size: 1827 1012 467 449 346 335 246 86 74 23 12 BtgI C'CryG_G Cuts at: 107 320 603 1046 1591 107 Size: 213 283 443 545 3393 Fragments arranged by size: 3393 545 443 283 213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 433 2827 3008 3171 433 Size: 2394 181 163 2139 Fragments arranged by size: 2394 2139 181 163 BtsI GCAGTG_nn' Cuts at: 508 556 2120 508 Size: 48 1564 3265 Fragments arranged by size: 3265 1564 48 ClaI AT'CG_AT Cuts at: 1634 1634 Size: 4877 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 771 806 771 Size: 35 4842 DraI TTT'AAA Cuts at: 1876 1876 Size: 4877 DraIII CAC_nnn'GTG Cuts at: 1118 1118 Size: 4877 DrdI GACnn_nn'nnGTC Cuts at: 1426 2583 3441 1426 Size: 1157 858 2862 Fragments arranged by size: 2862 1157 858 EaeI y'GGCC_r Cuts at: 194 224 413 701 814 1133 1535 1693 2462 Size: 30 189 288 113 319 402 158 769 Cuts at: 2462 2636 3027 3054 4399 194 Size: 174 391 27 1345 672 Fragments arranged by size: 1345 769 672 402 391 319 288 189 174 158 113 30 27 EagI C'GGCC_G Cuts at: 2462 2462 Size: 4877 EarI CTCTTCn'nnn_ Cuts at: 1294 1508 2900 3110 1294 Size: 214 1392 210 3061 Fragments arranged by size: 3061 1392 214 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3085 3395 3541 4687 3085 Size: 310 146 1146 3275 Fragments arranged by size: 3275 1146 310 146 EcoICRI GAG'CTC Cuts at: 1889 1889 Size: 4877 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 271 639 1541 2702 3134 3881 4149 271 Size: 368 902 1161 432 747 268 999 Fragments arranged by size: 1161 999 902 747 432 368 268 EcoO109I rG'GnC_Cy Cuts at: 800 1248 1323 1428 1449 1459 1585 1597 1730 Size: 448 75 105 21 10 126 12 133 Cuts at: 1730 1836 2016 800 Size: 106 180 3661 Fragments arranged by size: 3661 448 180 133 126 106 105 75 21 12 10 EcoRI G'AATT_C Cuts at: 1881 1881 Size: 4877 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2896 2896 Size: 4877 FspI TGC'GCA Cuts at: 1111 2658 1111 Size: 1547 3330 FspAI rTGC'GCAy Cuts at: 1111 1111 Size: 4877 HaeII r_GCGC'y Cuts at: 190 412 594 932 1012 1095 2311 2559 3581 Size: 222 182 338 80 83 1216 248 1022 Cuts at: 3581 4560 190 Size: 979 507 Fragments arranged by size: 1216 1022 979 507 338 248 222 182 83 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2719 2749 2751 2781 2719 Size: 30 2 30 4815 Fragments arranged by size: 4815 30 30 2 HincII GTy'rAC Cuts at: 477 1916 477 Size: 1439 3438 KasI G'GCGC_C Cuts at: 1008 2307 2555 1008 Size: 1299 248 3330 Fragments arranged by size: 3330 1299 248 KpnI G_GTAC'C Cuts at: 812 832 1897 812 Size: 20 1065 3792 Fragments arranged by size: 3792 1065 20 MluI A'CGCG_T Cuts at: 4310 4310 Size: 4877 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 176 374 980 1472 1681 1731 3548 3732 176 Size: 198 606 492 209 50 1817 184 1321 Fragments arranged by size: 1817 1321 606 492 209 198 184 50 MscI TGG'CCA Cuts at: 196 415 703 1135 1537 2638 196 Size: 219 288 432 402 1101 2435 Fragments arranged by size: 2435 1101 432 402 288 219 MslI CAynn'nnrTG Cuts at: 289 457 532 841 961 973 2866 2993 289 Size: 168 75 309 120 12 1893 127 2173 Fragments arranged by size: 2173 1893 309 168 127 120 75 12 MspA1I CmG'CkG Cuts at: 298 330 605 1863 1988 2302 2662 3675 3920 Size: 32 275 1258 125 314 360 1013 245 Cuts at: 3920 298 Size: 1255 Fragments arranged by size: 1258 1255 1013 360 314 275 245 125 32 NaeI GCC'GGC Cuts at: 3058 3058 Size: 4877 NarI GG'CG_CC Cuts at: 1009 2308 2556 1009 Size: 1299 248 3330 Fragments arranged by size: 3330 1299 248 NcoI C'CATG_G Cuts at: 107 1046 107 Size: 939 3938 NgoMIV G'CCGG_C Cuts at: 3056 3056 Size: 4877 NspI r_CATG'y Cuts at: 456 517 1287 1930 2430 3337 456 Size: 61 770 643 500 907 1996 Fragments arranged by size: 1996 907 770 643 500 61 PciI A'CATG_T Cuts at: 3333 3333 Size: 4877 PflMI CCAn_nnn'nTGG Cuts at: 203 572 1554 203 Size: 369 982 3526 Fragments arranged by size: 3526 982 369 PfoI T'CCnGG_A Cuts at: 1610 1610 Size: 4877 PmeI GTTT'AAAC Cuts at: 1876 1876 Size: 4877 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 4016 Size: 32 4845 PpuMI rG'GwC_Cy Cuts at: 1248 1323 1428 1459 1585 1597 1730 1248 Size: 75 105 31 126 12 133 4395 Fragments arranged by size: 4395 133 126 105 75 31 12 PspOMI G'GGCC_C Cuts at: 728 1448 1836 728 Size: 720 388 3769 Fragments arranged by size: 3769 720 388 PstI C_TGCA'G Cuts at: 911 1924 2609 911 Size: 1013 685 3179 Fragments arranged by size: 3179 1013 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4877 PvuII CAG'CTG Cuts at: 298 330 1863 2302 2662 298 Size: 32 1533 439 360 2513 Fragments arranged by size: 2513 1533 439 360 32 RsrII CG'GwC_CG Cuts at: 3072 3072 Size: 4877 SacI G_AGCT'C Cuts at: 1891 1891 Size: 4877 SacII CC_GC'GG Cuts at: 606 606 Size: 4877 SalI G'TCGA_C Cuts at: 1914 1914 Size: 4877 SanDI GG'GwC_CC Cuts at: 1248 1585 1730 1248 Size: 337 145 4395 Fragments arranged by size: 4395 337 145 SapI GCTCTTCn'nnn_ Cuts at: 1294 2900 3110 1294 Size: 1606 210 3061 Fragments arranged by size: 3061 1606 210 SbfI CC_TGCA'GG Cuts at: 911 1924 911 Size: 1013 3864 ScaI AGT'ACT Cuts at: 4394 4394 Size: 4877 SfcI C'TryA_G Cuts at: 33 907 1920 2605 3598 3789 33 Size: 874 1013 685 993 191 1121 Fragments arranged by size: 1121 1013 993 874 685 191 SfoI GGC'GCC Cuts at: 1010 2309 2557 1010 Size: 1299 248 3330 Fragments arranged by size: 3330 1299 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4877 SmaI CCC'GGG Cuts at: 1899 1899 Size: 4877 SmlI C'TyrA_G Cuts at: 994 1300 2032 2947 3439 3701 994 Size: 306 732 915 492 262 2170 Fragments arranged by size: 2170 915 732 492 306 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4877 SphI G_CATG'C Cuts at: 517 1930 2430 517 Size: 1413 500 2964 Fragments arranged by size: 2964 1413 500 StuI AGG'CCT Cuts at: 139 139 Size: 4877 StyI C'CwwG_G Cuts at: 107 803 1046 1725 2011 2365 107 Size: 696 243 679 286 354 2619 Fragments arranged by size: 2619 696 679 354 286 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2460 2593 3052 3261 2460 Size: 133 459 209 4076 Fragments arranged by size: 4076 459 209 133 TatI w'GTAC_w Cuts at: 4392 4392 Size: 4877 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4394 4426 4394 Size: 32 4845 Tth111I GACn'n_nGTC Cuts at: 2674 2674 Size: 4877 XbaI T'CTAG_A Cuts at: 1908 1908 Size: 4877 XcmI CCAnnnn_n'nnnnTGG Cuts at: 318 2372 318 Size: 2054 2823 XmaI C'CCGG_G Cuts at: 1897 1897 Size: 4877 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AflII AhdI AlfI AloI AscI AvrII BfrBI BmtI BsaXI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI