Restriction map for pF1KB9434
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HgaI        
Hin1I       HindIII     HinfI       HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        TfiI        Tru9I       Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcsI           58  AAATTT               [GA]^AATT[CT]
Aor51HI       330  AGCGCT               AGC^GCT
ApaI          276  GGGCCC               GGGCC^C
ApoI           58  AAATTT               [GA]^AATT[CT]
Asp700I       397  GAAATATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          511  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          117  CTCGGG               C^[CT]CG[GA]G
BanI          226  GGCACC               G^G[CT][GA]CC
BfaI          610  CTAG                 C^TAG
BglII         446  AGATCT               A^GATCT
BsiEI         510  CGACCG               CG[GA][CT]^CG
BsoBI         117  CTCGGG               C^[CT]CG[GA]G
BsrBI         250  GAGCGG               CCGCTC(-3/-3)
BstNI          67  CCTGG                CC^[AT]GG
Cac8I         336  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI          331  GCGC                 GCG^C
DdeI          101  CTCAG                C^T[ACGT]AG
Eco47III      330  AGCGCT               AGC^GCT
EcoNI          12  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII         67  CCTGG                ^CC[AT]GG
HaeII         330  AGCGCT               [GA]GCGC^[CT]
HhaI          331  GCGC                 GCG^C
HinP1I        331  GCGC                 G^CGC
HincII        544  GTCAAC               GT[CT]^[GA]AC
HindII        544  GTCAAC               GT[CT]^[GA]AC
MaeI          610  CTAG                 C^TAG
MaeII         491  ACGT                 A^CGT
MvaI           67  CCTGG                CC^[AT]GG
PflFI         511  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SfaNI         106  GCATC                GCATC(5/9)
SmlI          310  CTCAAG               C^T[CT][GA]AG
SspI          399  AATATT               AAT^ATT
TaqI          509  TCGA                 T^CGA
Tsp509I        59  AATT                 ^AATT
TspRI         153  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       511  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       509  TCGA                 T^CGA
XmnI          397  GAAATATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI          184  GTAC                 GT^AC
              348  GTAC                
AflIII        186  ACATGT               A^C[GA][CT]GT
              409  ACATGT              
AlwI          459  GATCC                GGATC(4/5)
              209  GGATC               
BanII          98  GGGCTC               G[GA]GC[CT]^C
              276  GGGCCC              
BbvI           87  GCTGC                GCAGC(8/12)
              333  GCTGC               
BcnI          477  CCGGG                CC^[GC]GG
              513  CCGGG               
BmyI           98  GGGCTC               G[AGT]GC[ACT]^C
              276  GGGCCC              
Bsp1286I       98  GGGCTC               G[AGT]GC[ACT]^C
              276  GGGCCC              
BspLU11I      186  ACATGT               A^CATGT
              409  ACATGT              
BspMI         550  ACCTGC               ACCTGC(4/8)
               18  GCAGGT              
BstYI         446  AGATCT               [GA]^GATC[CT]
              458  AGATCC              
DraII         275  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              276  GGGCCCC             
EcoO109I      275  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              276  GGGCCCC             
HaeIII        277  GGCC                 GG^CC
              537  GGCC                
HapII         477  CCGG                 C^CGG
              513  CCGG                
HpaII         477  CCGG                 C^CGG
              513  CCGG                
MaeIII        577  GTCAC                ^GT[ACGT]AC
              644  GTCAC               
MboII         456  GAAGA                GAAGA(8/7)
              200  TCTTC               
MflI          446  AGATCT               [GA]^GATC[CT]
              458  AGATCC              
MslI          519  CATTGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              579  CACAGAGATG          
MspI          477  CCGG                 C^CGG
              513  CCGG                
MwoI          324  GCCAACAGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              331  GCGCTGCCAGC         
NciI          477  CCGGG                CC^[GC]GG
              513  CCGGG               
NspI          186  ACATGT               [GA]CATG^[CT]
              409  ACATGT              
RsaI          184  GTAC                 GT^AC
              348  GTAC                
TseI           87  GCTGC                G^C[AT]GC
              333  GCTGC               
Tsp45I        577  GTCAC                ^GT[GC]AC
              644  GTCAC               
XhoII         446  AGATCT               [GA]^GATC[CT]
              458  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AciI        AluI        AvaII       BsgI        BsiYI       
BslI        BsrI        BssKI       BstF5I      Cfr13I      
DpnII       Fnu4HI      FokI        ItaI        MboI        
MnlI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       
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