(Circular) (Six-base) MAPSORT of: pF1KB9171.seq Check: 3815 from: 1 to: 5120 pF1KB9171 5120 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 848 966 1518 848 Size: 118 552 4450 Fragments arranged by size: 4450 552 118 AatII G_ACGT'C Cuts at: 1394 2039 1394 Size: 645 4475 AccI GT'mk_AC Cuts at: 2158 2158 Size: 5120 Acc65I G'GTAC_C Cuts at: 2136 2136 Size: 5120 AclI AA'CG_TT Cuts at: 4960 4960 Size: 5120 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2945 3377 4124 4392 2945 Size: 432 747 268 3673 Fragments arranged by size: 3673 747 432 268 AflIII A'CryG_T Cuts at: 2472 3576 4553 2472 Size: 1104 977 3039 Fragments arranged by size: 3039 1104 977 AgeI A'CCGG_T Cuts at: 1762 3508 1762 Size: 1746 3374 AlwNI CAG_nnn'CTG Cuts at: 363 433 972 981 1068 1561 1623 2046 2425 Size: 70 539 9 87 493 62 423 379 Cuts at: 2425 3992 363 Size: 1567 1491 Fragments arranged by size: 1567 1491 539 493 423 379 87 70 62 9 ApaI G_GGCC'C Cuts at: 1870 1870 Size: 5120 ApaLI G'TGCA_C Cuts at: 295 625 3890 295 Size: 330 3265 1525 Fragments arranged by size: 3265 1525 330 ApoI r'AATT_y Cuts at: 2124 4473 4729 2124 Size: 2349 256 2515 Fragments arranged by size: 2515 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5120 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5120 AvaI C'yCGr_G Cuts at: 169 396 502 1463 1973 2140 169 Size: 227 106 961 510 167 3149 Fragments arranged by size: 3149 961 510 227 167 106 AvrII C'CTAG_G Cuts at: 339 2004 339 Size: 1665 3455 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 384 417 384 Size: 33 5087 BamHI G'GATC_C Cuts at: 602 641 1964 2145 602 Size: 39 1323 181 3577 Fragments arranged by size: 3577 1323 181 39 BanI G'GyrC_C Cuts at: 506 689 1107 1992 2136 2550 2798 2833 506 Size: 183 418 885 144 414 248 35 2793 Fragments arranged by size: 2793 885 418 414 248 183 144 35 BanII G_rGCy'C Cuts at: 1213 1297 1617 1870 2134 3164 1213 Size: 84 320 253 264 1030 3169 Fragments arranged by size: 3169 1030 320 264 253 84 BbeI G_GCGC'C Cuts at: 1111 2554 2802 1111 Size: 1443 248 3429 Fragments arranged by size: 3429 1443 248 BciVI GTATCCnnnnn_n' Cuts at: 324 3018 3779 5120 324 Size: 2694 761 1341 324 Fragments arranged by size: 2694 1341 761 324 BclI T'GATC_A Cuts at: 2640 2640 Size: 5120 BglI GCCn_nnn'nGGC Cuts at: 268 430 504 1865 268 Size: 162 74 1361 3523 Fragments arranged by size: 3523 1361 162 74 BglII A'GATC_T Cuts at: 2635 4672 2635 Size: 2037 3083 BlpI GC'TnA_GC Cuts at: 2232 2232 Size: 5120 Bme1580I G_kGCm'C Cuts at: 299 509 629 1125 1146 1870 2745 2838 3894 Size: 210 120 496 21 724 875 93 1056 Cuts at: 3894 299 Size: 1525 Fragments arranged by size: 1525 1056 875 724 496 210 120 93 21 BmrI ACTGGGnnnn_n' Cuts at: 627 2332 2413 2748 4892 627 Size: 1705 81 335 2144 855 Fragments arranged by size: 2144 1705 855 335 81 BmtI G_CTAG'C Cuts at: 1960 1960 Size: 5120 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1959 3487 1959 Size: 1528 3592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 485 1030 1048 1162 1538 1643 1954 2089 485 Size: 545 18 114 376 105 311 135 3516 Fragments arranged by size: 3516 545 376 311 135 114 105 18 Bpu10I CC'TnA_GC Cuts at: 191 872 1774 191 Size: 681 902 3537 Fragments arranged by size: 3537 902 681 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2296 3175 3667 3965 2296 Size: 879 492 298 3451 Fragments arranged by size: 3451 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 322 891 4824 322 Size: 569 3933 618 Fragments arranged by size: 3933 618 569 BsaAI yAC'GTr Cuts at: 822 2473 822 Size: 1651 3469 BsaBI GATnn'nnATC Cuts at: 6 2634 4671 6 Size: 2628 2037 455 Fragments arranged by size: 2628 2037 455 BsaHI Gr'CG_yC Cuts at: 380 1108 1391 2036 2551 2799 3501 4997 380 Size: 728 283 645 515 248 702 1496 503 Fragments arranged by size: 1496 728 702 645 515 503 283 248 BsaWI w'CCGG_w Cuts at: 367 1762 2309 2830 3508 3782 3929 4340 367 Size: 1395 547 521 678 274 147 411 1147 Fragments arranged by size: 1395 1147 678 547 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1033 1063 2062 2092 1033 Size: 30 999 30 4061 Fragments arranged by size: 4061 999 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 295 364 1438 1483 1695 1951 295 Size: 69 1074 45 212 256 3464 Fragments arranged by size: 3464 1074 256 212 69 45 BseYI C'CCAG_C Cuts at: 285 667 1071 1213 1842 1952 2545 3303 3880 Size: 382 404 142 629 110 593 758 577 Cuts at: 3880 4376 285 Size: 496 1029 Fragments arranged by size: 1029 758 629 593 577 496 404 382 142 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 554 820 1271 1466 1862 554 Size: 266 451 195 396 3812 Fragments arranged by size: 3812 451 396 266 195 BsiEI CG_ry'CG Cuts at: 104 993 2662 2708 3916 104 Size: 889 1669 46 1208 1308 Fragments arranged by size: 1669 1308 1208 889 46 BsiHKAI G_wGCw'C Cuts at: 299 461 629 1297 2134 2912 3102 3894 299 Size: 162 168 668 837 778 190 792 1525 Fragments arranged by size: 1525 837 792 778 668 190 168 162 Bsp1286I G_dGCh'C Cuts at: 299 461 509 629 1125 1146 1213 1297 1617 Size: 162 48 120 496 21 67 84 320 Cuts at: 1617 1870 2134 2745 2838 2912 3102 3164 3894 Size: 253 264 611 93 74 190 62 730 Cuts at: 3894 299 Size: 1525 Fragments arranged by size: 1525 730 611 496 320 264 253 190 162 120 93 84 74 67 62 48 21 BspEI T'CCGG_A Cuts at: 367 4340 367 Size: 3973 1147 BspHI T'CATG_A Cuts at: 4296 4296 Size: 5120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 791 848 966 1518 2170 2384 2686 3067 791 Size: 57 118 552 652 214 302 381 2844 Fragments arranged by size: 2844 652 552 381 302 214 118 57 BsrBI CCG'CTC Cuts at: 50 3412 3466 4950 5115 50 Size: 3362 54 1484 165 55 Fragments arranged by size: 3362 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1898 3032 1898 Size: 1134 3986 BsrFI r'CCGG_y Cuts at: 985 1652 1762 3118 3299 3508 985 Size: 667 110 1356 181 209 2597 Fragments arranged by size: 2597 1356 667 209 181 110 BssHII G'CGCG_C Cuts at: 413 413 Size: 5120 BssSI C'ACGA_G Cuts at: 1450 3391 3749 1450 Size: 1941 358 2821 Fragments arranged by size: 2821 1941 358 BstAPI GCAn_nnn'nTGC Cuts at: 843 2497 843 Size: 1654 3466 BstBI TT'CG_AA Cuts at: 3481 3481 Size: 5120 BstEII G'GTnAC_C Cuts at: 1075 1075 Size: 5120 BstYI r'GATC_y Cuts at: 1 602 641 1964 2145 2612 2635 2970 3216 Size: 601 39 1323 181 467 23 335 246 Cuts at: 3216 4228 4314 4326 4672 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1323 1012 601 467 449 346 335 246 181 86 39 23 12 Bsu36I CC'TnA_GG Cuts at: 920 1417 920 Size: 497 4623 BtgI C'CryG_G Cuts at: 107 510 630 1608 1854 107 Size: 403 120 978 246 3373 Fragments arranged by size: 3373 978 403 246 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3070 3251 3414 3070 Size: 181 163 4776 Fragments arranged by size: 4776 181 163 BtsI GCAGTG_nn' Cuts at: 408 752 1478 1747 2098 2363 408 Size: 344 726 269 351 265 3165 Fragments arranged by size: 3165 726 351 344 269 265 DraI TTT'AAA Cuts at: 2119 2119 Size: 5120 DraIII CAC_nnn'GTG Cuts at: 2109 2109 Size: 5120 DrdI GACnn_nn'nnGTC Cuts at: 670 2826 3684 670 Size: 2156 858 2106 Fragments arranged by size: 2156 2106 858 EaeI y'GGCC_r Cuts at: 143 422 513 557 633 983 990 1851 2705 Size: 279 91 44 76 350 7 861 854 Cuts at: 2705 2879 3270 3297 4642 143 Size: 174 391 27 1345 621 Fragments arranged by size: 1345 861 854 621 391 350 279 174 91 76 44 27 7 EagI C'GGCC_G Cuts at: 990 2705 990 Size: 1715 3405 EarI CTCTTCn'nnn_ Cuts at: 272 1165 3143 3353 272 Size: 893 1978 210 2039 Fragments arranged by size: 2039 1978 893 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1054 3328 3638 3784 4930 1054 Size: 2274 310 146 1146 1244 Fragments arranged by size: 2274 1244 1146 310 146 EcoICRI GAG'CTC Cuts at: 1295 2132 1295 Size: 837 4283 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 485 1030 1048 1162 1538 1643 1954 2089 2945 Size: 545 18 114 376 105 311 135 856 Cuts at: 2945 3377 4124 4392 485 Size: 432 747 268 1213 Fragments arranged by size: 1213 856 747 545 432 376 311 268 135 114 105 18 EcoNI CCTnn'n_nnAGG Cuts at: 343 1420 1440 1933 2003 343 Size: 1077 20 493 70 3460 Fragments arranged by size: 3460 1077 493 70 20 EcoO109I rG'GnC_Cy Cuts at: 336 762 915 1158 1473 1846 1866 1867 2008 Size: 426 153 243 315 373 20 1 141 Cuts at: 2008 2043 2259 336 Size: 35 216 3197 Fragments arranged by size: 3197 426 373 315 243 216 153 141 35 20 1 EcoRI G'AATT_C Cuts at: 2124 2124 Size: 5120 EcoRV GAT'ATC Cuts at: 1368 1368 Size: 5120 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3139 3139 Size: 5120 FspI TGC'GCA Cuts at: 2901 2901 Size: 5120 HaeII r_GCGC'y Cuts at: 198 364 711 1111 1843 2554 2802 3824 4803 Size: 166 347 400 732 711 248 1022 979 Cuts at: 4803 198 Size: 515 Fragments arranged by size: 1022 979 732 711 515 400 347 248 166 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 171 203 298 330 633 665 749 781 2962 Size: 32 95 32 303 32 84 32 2181 Cuts at: 2962 2992 2994 3024 171 Size: 30 2 30 2267 Fragments arranged by size: 2267 2181 303 95 84 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 780 2159 780 Size: 1379 3741 KasI G'GCGC_C Cuts at: 1107 2550 2798 1107 Size: 1443 248 3429 Fragments arranged by size: 3429 1443 248 KpnI G_GTAC'C Cuts at: 2140 2140 Size: 5120 MluI A'CGCG_T Cuts at: 4553 4553 Size: 5120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1422 3791 3975 1422 Size: 2369 184 2567 Fragments arranged by size: 2567 2369 184 MscI TGG'CCA Cuts at: 515 559 635 1853 2881 515 Size: 44 76 1218 1028 2754 Fragments arranged by size: 2754 1218 1028 76 44 MslI CAynn'nnrTG Cuts at: 695 3109 3236 695 Size: 2414 127 2579 Fragments arranged by size: 2579 2414 127 MspA1I CmG'CkG Cuts at: 444 568 969 1198 1279 1345 1647 2067 2231 Size: 124 401 229 81 66 302 420 164 Cuts at: 2231 2545 2905 3918 4163 444 Size: 314 360 1013 245 1401 Fragments arranged by size: 1401 1013 420 401 360 314 302 245 229 164 124 81 66 NaeI GCC'GGC Cuts at: 987 3301 987 Size: 2314 2806 NarI GG'CG_CC Cuts at: 1108 2551 2799 1108 Size: 1443 248 3429 Fragments arranged by size: 3429 1443 248 NcoI C'CATG_G Cuts at: 107 510 630 1854 107 Size: 403 120 1224 3373 Fragments arranged by size: 3373 1224 403 120 NgoMIV G'CCGG_C Cuts at: 985 3299 985 Size: 2314 2806 NheI G'CTAG_C Cuts at: 1956 1956 Size: 5120 NspI r_CATG'y Cuts at: 577 2173 2673 3580 577 Size: 1596 500 907 2117 Fragments arranged by size: 2117 1596 907 500 PasI CC'CwG_GG Cuts at: 1638 1638 Size: 5120 PciI A'CATG_T Cuts at: 3576 3576 Size: 5120 PfoI T'CCnGG_A Cuts at: 1301 1931 1301 Size: 630 4490 PmeI GTTT'AAAC Cuts at: 2119 2119 Size: 5120 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 4259 Size: 32 5088 PpuMI rG'GwC_Cy Cuts at: 336 915 336 Size: 579 4541 PshAI GACnn'nnGTC Cuts at: 334 334 Size: 5120 PspOMI G'GGCC_C Cuts at: 1866 1866 Size: 5120 PstI C_TGCA'G Cuts at: 359 443 977 1569 1865 2167 2852 359 Size: 84 534 592 296 302 685 2627 Fragments arranged by size: 2627 685 592 534 302 296 84 PvuI CG_AT'CG Cuts at: 104 104 Size: 5120 PvuII CAG'CTG Cuts at: 444 568 969 1279 1647 2067 2545 2905 444 Size: 124 401 310 368 420 478 360 2659 Fragments arranged by size: 2659 478 420 401 368 360 310 124 RsrII CG'GwC_CG Cuts at: 3315 3315 Size: 5120 SacI G_AGCT'C Cuts at: 1297 2134 1297 Size: 837 4283 SalI G'TCGA_C Cuts at: 2157 2157 Size: 5120 SanDI GG'GwC_CC Cuts at: 915 915 Size: 5120 SapI GCTCTTCn'nnn_ Cuts at: 1165 3143 3353 1165 Size: 1978 210 2932 Fragments arranged by size: 2932 1978 210 SbfI CC_TGCA'GG Cuts at: 1865 2167 1865 Size: 302 4818 ScaI AGT'ACT Cuts at: 4637 4637 Size: 5120 SexAI A'CCwGG_T Cuts at: 794 1826 2105 794 Size: 1032 279 3809 Fragments arranged by size: 3809 1032 279 SfcI C'TryA_G Cuts at: 33 355 439 973 1565 1861 2163 2848 3841 Size: 322 84 534 592 296 302 685 993 Cuts at: 3841 4032 33 Size: 191 1121 Fragments arranged by size: 1121 993 685 592 534 322 302 296 191 84 SfiI GGCCn_nnn'nGGCC Cuts at: 1865 1865 Size: 5120 SfoI GGC'GCC Cuts at: 1109 2552 2800 1109 Size: 1443 248 3429 Fragments arranged by size: 3429 1443 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5120 SmaI CCC'GGG Cuts at: 504 1465 1975 2142 504 Size: 961 510 167 3482 Fragments arranged by size: 3482 961 510 167 SmlI C'TyrA_G Cuts at: 2275 3190 3682 3944 2275 Size: 915 492 262 3451 Fragments arranged by size: 3451 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5120 SphI G_CATG'C Cuts at: 2173 2673 2173 Size: 500 4620 StuI AGG'CCT Cuts at: 271 498 1239 1601 271 Size: 227 741 362 3790 Fragments arranged by size: 3790 741 362 227 StyI C'CwwG_G Cuts at: 107 339 510 630 1854 2004 2254 2608 107 Size: 232 171 120 1224 150 250 354 2619 Fragments arranged by size: 2619 1224 354 250 232 171 150 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1063 2703 2836 3295 3504 1063 Size: 1640 133 459 209 2679 Fragments arranged by size: 2679 1640 459 209 133 TatI w'GTAC_w Cuts at: 1059 4635 1059 Size: 3576 1544 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 229 261 1033 1065 1297 1319 1329 1351 2062 Size: 32 772 32 232 22 10 22 711 Cuts at: 2062 2094 4637 4669 229 Size: 32 2543 32 680 Fragments arranged by size: 2543 772 711 680 232 32 32 32 32 22 22 10 Tth111I GACn'n_nGTC Cuts at: 2917 2917 Size: 5120 XbaI T'CTAG_A Cuts at: 2151 2151 Size: 5120 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1581 2615 1581 Size: 1034 4086 XmaI C'CCGG_G Cuts at: 502 1463 1973 2140 502 Size: 961 510 167 3482 Fragments arranged by size: 3482 961 510 167 ZraI GAC'GTC Cuts at: 1392 2037 1392 Size: 645 4475 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AfeI AflII AhdI AleI AlfI AloI AscI BbsI BbvCI BcgI BfrBI BmgBI BsiWI BsmI BsmBI BsrGI BstXI BstZ17I ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI PacI PflMI PmlI PsiI PspXI PsrI SacII SgrAI SnaBI SrfI SspI SwaI XhoI XmnI