################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbvCI BcgI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HindIII HpaI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcyI 352 GGCGCC G[GA]^CG[CT]C AfaI 489 GTAC GT^AC ApaI 604 GGGCCC GGGCC^C BanI 352 GGCGCC G^G[CT][GA]CC BanII 604 GGGCCC G[GA]GC[CT]^C BbeI 352 GGCGCC GGCGC^C BbrPI 79 CACGTG CAC^GTG BbsI 774 GAAGAC GAAGAC(2/6) BciVI 571 GGATAC GTATCC(6/5) BclI 75 TGATCA T^GATCA BcnI 165 CCGGG CC^[GC]GG BglI 497 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 604 GGGCCC G[AGT]GC[ACT]^C BpuAI 774 GAAGAC GAAGAC(2/6) BsaHI 352 GGCGCC G[GA]^CG[CT]C BsgI 577 GTGCAG GTGCAG(16/14) Bsp1286I 604 GGGCCC G[AGT]GC[ACT]^C BssSI 729 CACGAG CACGAG(-5/-1) DdeI 530 CTGAG C^T[ACGT]AG FbaI 75 TGATCA T^GATCA Hin1I 352 GGCGCC G[GA]^CG[CT]C HincII 333 GTTGAC GT[CT]^[GA]AC HindII 333 GTTGAC GT[CT]^[GA]AC HinfI 15 GAATC G^A[ACGT]TC HphI 406 GGTGA GGTGA(8/7) KasI 352 GGCGCC G^GCGCC MslI 60 CATCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 352 GGCGCC GG^CGCC NciI 165 CCGGG CC^[GC]GG PmaCI 79 CACGTG CAC^GTG PmlI 79 CACGTG CAC^GTG PpuMI 543 AGGACCC [GA]G^G[AT]CC[CT] PstI 650 CTGCAG CTGCA^G RsaI 489 GTAC GT^AC SfcI 650 CTGCAG C^T[GA][CT]AG SfoI 352 GGCGCC GGC^GCC SmlI 392 CTCAAG C^T[CT][GA]AG TfiI 15 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccI 148 GTCTAC GT^[AC][GT]AC 718 GTCTAC AvaII 131 GGACC G^G[AT]CC 544 GGACC BpmI 379 CTCCAG CTGGAG(16/14) 399 CTCCAG BsaAI 79 CACGTG [CT]AC^GT[GA] 574 TACGTG BsaWI 618 TCCGGT [AT]^CCGG[AT] 762 ACCGGA BspHI 209 TCATGA T^CATGA 227 TCATGA BsrI 416 CCAGT ACTGG(1/-1) 472 CCAGT Cac8I 682 GCCAGC GC[ACGT]^[ACGT]GC 734 GCCAGC DraII 451 GGGGCCT [GA]G^G[ACGT]CC[CT] 543 AGGACCC DsaI 566 CCATGG C^C[GA][CT]GG 608 CCATGG EcoO109I 451 GGGGCCT [GA]G^G[ACGT]CC[CT] 543 AGGACCC NcoI 566 CCATGG C^CATGG 608 CCATGG NspI 440 ACATGC [GA]CATG^[CT] 722 ACATGC RcaI 209 TCATGA T^CATGA 227 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI CfoI Cfr13I DpnII EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI