################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MseI MslI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 3 GACGCC G[GA]^CG[CT]C AfaI 495 GTAC GT^AC Alw44I 313 GTGCAC G^TGCAC AlwNI 895 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 339 CCTGAGG CC^T[ACGT]AGG ApaLI 313 GTGCAC G^TGCAC AspHI 313 GTGCAC G[AT]GC[AT]^C AviII 134 TGCGCA TGC^GCA AvrII 798 CCTAGG C^CTAGG BglI 762 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 798 CCTAGG C^CTAGG BsaAI 715 TACGTG [CT]AC^GT[GA] BsaHI 3 GACGCC G[GA]^CG[CT]C BsgI 812 GTGCAG GTGCAG(16/14) BsiHKAI 313 GTGCAC G[AT]GC[AT]^C BsmI 365 GCATTC GAATGC(1/-1) BsrFI 596 GCCGGC [GA]^CCGG[CT] Bsu36I 339 CCTGAGG CC^T[ACGT]AGG Cfr10I 596 GCCGGC [GA]^CCGG[CT] DpnII 847 GATC ^GATC DrdI 777 GACACTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 339 CCTGAGG CC^T[ACGT]AGG FspI 134 TGCGCA TGC^GCA HgaI 3 GACGC GACGC(5/10) Hin1I 3 GACGCC G[GA]^CG[CT]C HincII 970 GTCGAC GT[CT]^[GA]AC HindII 970 GTCGAC GT[CT]^[GA]AC HinfI 228 GAGTC G^A[ACGT]TC MaeII 716 ACGT A^CGT MaeIII 239 GTTAC ^GT[ACGT]AC MboI 847 GATC ^GATC NaeI 596 GCCGGC GCC^GGC NcoI 833 CCATGG C^CATGG NdeII 847 GATC ^GATC NgoMIV 596 GCCGGC G^CCGGC PflMI 142 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 228 GAGTC GAGTC(4/5) PvuII 580 CAGCTG CAG^CTG RsaI 495 GTAC GT^AC SalI 970 GTCGAC G^TCGAC Sau3AI 847 GATC ^GATC SbfI 891 CCTGCAGG CCTGCA^GG SfaNI 113 GCATC GCATC(5/9) Sse8387I 891 CCTGCAGG CCTGCA^GG Van91I 142 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 259 GTCTAC GT^[AC][GT]AC 970 GTCGAC AluI 463 AGCT AG^CT 581 AGCT Aor51HI 323 AGCGCT AGC^GCT 851 AGCGCT ApaI 644 GGGCCC GGGCC^C 709 GGGCCC AvaI 641 CCCGGG C^[CT]CG[GA]G 917 CCCGGG BalI 563 TGGCCA TGG^CCA 719 TGGCCA BfaI 103 CTAG C^TAG 799 CTAG BsoBI 641 CCCGGG C^[CT]CG[GA]G 917 CCCGGG BspMI 95 ACCTGC ACCTGC(4/8) 144 ACCTGC BsrBI 422 CCGCTC CCGCTC(-3/-3) 604 GAGCGG DsaI 527 CCGTGG C^C[GA][CT]GG 833 CCATGG EaeI 563 TGGCCA [CT]^GGCC[GA] 719 TGGCCA EarI 691 CTCTTC CTCTTC(1/4) 908 GAAGAG Eco47III 323 AGCGCT AGC^GCT 851 AGCGCT HphI 86 TCACC GGTGA(8/7) 386 TCACC Ksp632I 691 CTCTTC CTCTTC(1/4) 908 GAAGAG MaeI 103 CTAG C^TAG 799 CTAG MluNI 563 TGGCCA TGG^CCA 719 TGGCCA MscI 563 TGGCCA TGG^CCA 719 TGGCCA MspA1I 580 CAGCTG C[AC]G^C[GT]G 815 CAGCGG SapI 908 GAAGAGC GCTCTTC(1/4) 690 GCTCTTC SmaI 641 CCCGGG CCC^GGG 917 CCCGGG TaqI 500 TCGA T^CGA 971 TCGA TthHB8I 500 TCGA T^CGA 971 TCGA XmaCI 641 CCCGGG C^CCGGG 917 CCCGGG XmaI 641 CCCGGG C^CCGGG 917 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrDI BsrI BssKI BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI Sau96I ScrFI SfcI StyI TseI TspRI XcmI