################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BglI BglII BlnI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstF5I BstUI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NarI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AflII 585 CTTAAG C^TTAAG AlwI 534 GATCC GGATC(4/5) AspHI 85 GTGCTC G[AT]GC[AT]^C BanI 123 GGTGCC G^G[CT][GA]CC BcnI 450 CCCGG CC^[GC]GG BfrI 585 CTTAAG C^TTAAG BlpI 159 GCTGAGC GC^T[ACGT]AGC BpmI 301 CTGGAG CTGGAG(16/14) Bpu1102I 159 GCTGAGC GC^T[ACGT]AGC BsaAI 271 TACGTG [CT]AC^GT[GA] BsiHKAI 85 GTGCTC G[AT]GC[AT]^C BspMI 389 ACCTGC ACCTGC(4/8) BssSI 439 CACGAG CACGAG(-5/-1) BstEII 330 GGTGACC G^GT[ACGT]ACC BstPI 330 GGTGACC G^GT[ACGT]ACC CelII 159 GCTGAGC GC^T[ACGT]AGC DdeI 160 CTGAG C^T[ACGT]AG DraIII 325 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 80 CCACGG C^C[GA][CT]GG EcoNI 501 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 330 GGTGACC G^GT[ACGT]ACC EcoT14I 176 CCAAGG C^C[AT][AT]GG HaeII 432 GGCGCT [GA]GCGC^[CT] MseI 586 TTAA T^TAA MslI 267 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 450 CCCGG CC^[GC]GG NlaIV 123 GGTGCC GG[ACGT]^[ACGT]CC NotI 225 GCGGCCGC GC^GGCCGC PpuMI 386 AGGACCT [GA]G^G[AT]CC[CT] SfcI 243 CTACAG C^T[GA][CT]AG StyI 176 CCAAGG C^C[AT][AT]GG Tru9I 586 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AvaII 294 GGACC G^G[AT]CC 387 GGACC BseRI 12 CTCCTC GAGGAG(10/8) 304 GAGGAG BsiEI 68 CGGCCG CG[GA][CT]^CG 226 CGGCCG BsrI 577 ACTGG ACTGG(1/-1) 33 CCAGT BstAPI 182 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 384 GCAGGACCTGC BstNI 213 CCAGG CC^[AT]GG 351 CCAGG CfoI 433 GCGC GCG^C 482 GCGC DpnII 153 GATC ^GATC 534 GATC DraII 386 AGGACCT [GA]G^G[ACGT]CC[CT] 497 AGGGCCT EagI 68 CGGCCG C^GGCCG 226 CGGCCG EclXI 68 CGGCCG C^GGCCG 226 CGGCCG Eco52I 68 CGGCCG C^GGCCG 226 CGGCCG EcoO109I 386 AGGACCT [GA]G^G[ACGT]CC[CT] 497 AGGGCCT EcoRII 213 CCAGG ^CC[AT]GG 351 CCAGG HhaI 433 GCGC GCG^C 482 GCGC HinP1I 433 GCGC G^CGC 482 GCGC HinfI 64 GACTC G^A[ACGT]TC 280 GACTC MaeIII 331 GTGAC ^GT[ACGT]AC 581 GTGAC MboI 153 GATC ^GATC 534 GATC MvaI 213 CCAGG CC^[AT]GG 351 CCAGG NaeI 70 GCCGGC GCC^GGC 474 GCCGGC NdeII 153 GATC ^GATC 534 GATC NgoMIV 70 GCCGGC G^CCGGC 474 GCCGGC NlaIII 51 CATG CATG^ 513 CATG PleI 64 GACTC GAGTC(4/5) 280 GACTC PvuII 186 CAGCTG CAG^CTG 427 CAGCTG Sau3AI 153 GATC ^GATC 534 GATC SfaNI 182 GCATC GCATC(5/9) 263 GCATC Tsp45I 331 GTGAC ^GT[GC]AC 581 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI AlwNI BbvI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI Cac8I Cfr10I Cfr13I EaeI Fnu4HI HaeIII HapII HpaII HphI ItaI MaeII MboII MnlI MspA1I MspI MwoI Sau96I ScrFI SmlI TseI