Restriction map for pF1KB8988
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BclI        BfaI        BfrI        BglII       
BlnI        BpuAI       BsaBI       BseAI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MspA1I      MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
XbaI        XhoII       


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI          991  GAATTC               [GA]^AATT[CT]
AflIII        584  ACACGT               A^C[GA][CT]GT
ApoI          991  GAATTC               [GA]^AATT[CT]
Asp700I       147  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          258  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI          986  GCAATGAATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         131  GTATCC               GTATCC(6/5)
BlpI          222  GCTCAGC              GC^T[ACGT]AGC
BpmI          637  CTGGAG               CTGGAG(16/14)
Bpu1102I      222  GCTCAGC              GC^T[ACGT]AGC
BsmBI         801  CGTCTC               CGTCTC(1/5)
BsrBI         295  GAGCGG               CCGCTC(-3/-3)
BsrDI         986  GCAATG               GCAATG(2/0)
BssSI         535  CACGAG               CACGAG(-5/-1)
BstAPI        309  GCAGCACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         222  GCTCAGC              GC^T[ACGT]AGC
DraII        1089  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I     1089  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         991  GAATTC               G^AATTC
HinfI        1027  GACTC                G^A[ACGT]TC
MaeIII       1078  GTGAC                ^GT[ACGT]AC
NcoI         1043  CCATGG               C^CATGG
PaeR7I         40  CTCGAG               C^TCGAG
PflFI         258  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         762  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1027  GACTC                GAGTC(4/5)
PpuMI        1089  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI          307  CTGCAG               CTGCA^G
ScaI          491  AGTACT               AGT^ACT
SmlI           40  CTCGAG               C^T[CT][GA]AG
Tsp45I       1078  GTGAC                ^GT[GC]AC
Tsp509I       992  AATT                 ^AATT
Tth111I       258  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        762  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI           40  CTCGAG               C^TCGAG
XmnI          147  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII         347  CGCG                 CG^CG
              382  CGCG                
AlwI          945  GGATC                GGATC(4/5)
              981  GGATC               
BanI          565  GGCACC               G^G[CT][GA]CC
              716  GGCACC              
BglI          518  GCCTCCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1033  GCCACAGGGGC         
BsaWI         729  ACCGGA               [AT]^CCGG[AT]
             1065  TCCGGT              
BseRI         660  CTCCTC               GAGGAG(10/8)
              676  GAGGAG              
BsgI          208  CTGCAC               GTGCAG(16/14)
              193  GTGCAG              
BsiEI          89  CGGCCG               CG[GA][CT]^CG
              343  CGGCCG              
BsiWI         278  CGTACG               C^GTACG
              593  CGTACG              
BspMI         206  ACCTGC               ACCTGC(4/8)
              707  ACCTGC              
BsrI          789  CCAGT                ACTGG(1/-1)
              999  CCAGT               
BstUI         347  CGCG                 CG^CG
              382  CGCG                
DpnII         946  GATC                 ^GATC
              982  GATC                
DsaI         1043  CCATGG               C^C[GA][CT]GG
             1130  CCACGG              
EagI           89  CGGCCG               C^GGCCG
              343  CGGCCG              
EarI          270  GAAGAG               CTCTTC(1/4)
              441  GAAGAG              
EclXI          89  CGGCCG               C^GGCCG
              343  CGGCCG              
Eco52I         89  CGGCCG               C^GGCCG
              343  CGGCCG              
FauI          692  CCCGC                CCCGC(4/6)
              912  CCCGC               
HaeII         252  GGCGCT               [GA]GCGC^[CT]
              462  GGCGCT              
Ksp632I       270  GAAGAG               CTCTTC(1/4)
              441  GAAGAG              
MboI          946  GATC                 ^GATC
              982  GATC                
MvnI          347  CGCG                 CG^CG
              382  CGCG                
NaeI           87  GCCGGC               GCC^GGC
              543  GCCGGC              
NdeII         946  GATC                 ^GATC
              982  GATC                
NgoMIV         87  GCCGGC               G^CCGGC
              543  GCCGGC              
NspI          314  ACATGC               [GA]CATG^[CT]
             1059  GCATGT              
SacI          220  GAGCTC               GAGCT^C
              405  GAGCTC              
Sau3AI        946  GATC                 ^GATC
              982  GATC                
XcmI          672  CCAGGAGGAGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1034  CCACAGGGGCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AspHI       
AvaI        AvaII       BalI        BanII       BbvI        
BcnI        BmyI        BsaAI       BsaHI       BsaI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        EaeI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       HphI        
ItaI        MaeII       MboII       MluNI       MnlI        
MscI        MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       RsaI        Sau96I      ScrFI       
SfaNI       SfcI        SmaI        StyI        TaqI        
TseI        TspRI       TthHB8I     XmaCI       XmaI        
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