Restriction map for pF1KB8980
 (Linear) (Six-base) MAPSORT of: KIBB8980.seq  Check: 5030  from: 1  to: 1128



KIBB8980.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AfeI AGC'GCT

Cuts at:      0     533    1128 
   Size:        533     595
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     268    1128 
   Size:        268     860
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1119    1128 
   Size:       1119       9
 
ApaI G_GGCC'C

Cuts at:      0     726    1128 
   Size:        726     402
 
ApoI r'AATT_y

Cuts at:      0      10     481    1128 
   Size:         10     471     647

  Fragments arranged by size: 

                 647     471      10
 
AvaI C'yCGr_G

Cuts at:      0     277    1128 
   Size:        277     851
 
BanI G'GyrC_C

Cuts at:      0     252     262     431    1128 
   Size:        252      10     169     697

  Fragments arranged by size: 

                 697     252     169      10
 
BanII G_rGCy'C

Cuts at:      0     229     726    1128 
   Size:        229     497     402

  Fragments arranged by size: 

                 497     402     229
 
BbeI G_GCGC'C

Cuts at:      0     266     435    1128 
   Size:        266     169     693

  Fragments arranged by size: 

                 693     266     169
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     694    1128 
   Size:        694     434
 
BbvCI CC'TCA_GC

Cuts at:      0     379    1128 
   Size:        379     749
 
BglI GCCn_nnn'nGGC

Cuts at:      0     233    1128 
   Size:        233     895
 
Bme1580I G_kGCm'C

Cuts at:      0     257     726    1128 
   Size:        257     469     402

  Fragments arranged by size: 

                 469     402     257
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     600     811    1019    1128 
   Size:        600     211     208     109

  Fragments arranged by size: 

                 600     211     208     109
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     975    1128 
   Size:        975     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751    1128 
   Size:        751     377
 
Bpu10I CC'TnA_GC

Cuts at:      0     379     709    1128 
   Size:        379     330     419

  Fragments arranged by size: 

                 419     379     330
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     970    1128 
   Size:        670     300     158

  Fragments arranged by size: 

                 670     300     158
 
BsaHI Gr'CG_yC

Cuts at:      0     263     432    1128 
   Size:        263     169     696

  Fragments arranged by size: 

                 696     263     169
 
BsaWI w'CCGG_w

Cuts at:      0     578    1128 
   Size:        578     550
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     137     685    1128 
   Size:        137     548     443

  Fragments arranged by size: 

                 548     443     137
 
BseYI C'CCAG_C

Cuts at:      0     169    1128 
   Size:        169     959
 
BsiEI CG_ry'CG

Cuts at:      0     213     335    1128 
   Size:        213     122     793

  Fragments arranged by size: 

                 793     213     122
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     720    1128 
   Size:        720     408
 
Bsp1286I G_dGCh'C

Cuts at:      0     229     257     726    1128 
   Size:        229      28     469     402

  Fragments arranged by size: 

                 469     402     229      28
 
BsrDI GCAATG_nn'

Cuts at:      0     843    1128 
   Size:        843     285
 
BsrFI r'CCGG_y

Cuts at:      0     259     330     364    1128 
   Size:        259      71      34     764

  Fragments arranged by size: 

                 764     259      71      34
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     205     913    1128 
   Size:        205     708     215

  Fragments arranged by size: 

                 708     215     205
 
Bsu36I CC'TnA_GG

Cuts at:      0     941    1128 
   Size:        941     187
 
BtgI C'CryG_G

Cuts at:      0     198     230     240    1128 
   Size:        198      32      10     888

  Fragments arranged by size: 

                 888     198      32      10
 
EaeI y'GGCC_r

Cuts at:      0     210     308     332    1128 
   Size:        210      98      24     796

  Fragments arranged by size: 

                 796     210      98      24
 
EagI C'GGCC_G

Cuts at:      0     210     332    1128 
   Size:        210     122     796

  Fragments arranged by size: 

                 796     210     122
 
EarI CTCTTCn'nnn_

Cuts at:      0     677    1128 
   Size:        677     451
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751    1128 
   Size:        751     377
 
EcoO109I rG'GnC_Cy

Cuts at:      0     722     723     945     954    1128 
   Size:        722       1     222       9     174

  Fragments arranged by size: 

                 722     222     174       9       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     711    1128 
   Size:        711     417
 
HaeII r_GCGC'y

Cuts at:      0     206     266     435     535    1128 
   Size:        206      60     169     100     593

  Fragments arranged by size: 

                 593     206     169     100      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     132     164    1128 
   Size:        132      32     964

  Fragments arranged by size: 

                 964     132      32
 
HincII GTy'rAC

Cuts at:      0     896    1128 
   Size:        896     232
 
KasI G'GCGC_C

Cuts at:      0     262     431    1128 
   Size:        262     169     697

  Fragments arranged by size: 

                 697     262     169
 
MspA1I CmG'CkG

Cuts at:      0     232     242     416    1066    1128 
   Size:        232      10     174     650      62

  Fragments arranged by size: 

                 650     232     174      62      10
 
NaeI GCC'GGC

Cuts at:      0     261     332    1128 
   Size:        261      71     796

  Fragments arranged by size: 

                 796     261      71
 
NarI GG'CG_CC

Cuts at:      0     263     432    1128 
   Size:        263     169     696

  Fragments arranged by size: 

                 696     263     169
 
NgoMIV G'CCGG_C

Cuts at:      0     259     330    1128 
   Size:        259      71     798

  Fragments arranged by size: 

                 798     259      71
 
NotI GC'GGCC_GC

Cuts at:      0     210    1128 
   Size:        210     918
 
PpuMI rG'GwC_Cy

Cuts at:      0     954    1128 
   Size:        954     174
 
PspOMI G'GGCC_C

Cuts at:      0     722    1128 
   Size:        722     406
 
PstI C_TGCA'G

Cuts at:      0     272    1004    1128 
   Size:        272     732     124

  Fragments arranged by size: 

                 732     272     124
 
PvuII CAG'CTG

Cuts at:      0    1066    1128 
   Size:       1066      62
 
SacII CC_GC'GG

Cuts at:      0     233     243    1128 
   Size:        233      10     885

  Fragments arranged by size: 

                 885     233      10
 
SfcI C'TryA_G

Cuts at:      0     268    1000    1128 
   Size:        268     732     128

  Fragments arranged by size: 

                 732     268     128
 
SfoI GGC'GCC

Cuts at:      0     264     433    1128 
   Size:        264     169     695

  Fragments arranged by size: 

                 695     264     169
 
SgrAI Cr'CCGG_yG

Cuts at:      0     259    1128 
   Size:        259     869
 
SmlI C'TyrA_G

Cuts at:      0     649     949    1128 
   Size:        649     300     179

  Fragments arranged by size: 

                 649     300     179
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     888    1038    1128 
   Size:        888     150      90

  Fragments arranged by size: 

                 888     150      90
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     376     408    1128 
   Size:        376      32     720

  Fragments arranged by size: 

                 720     376      32
 
XbaI T'CTAG_A

Cuts at:      0     465    1128 
   Size:        465     663
 

 Enzymes that do cut:

     AfeI     AlfI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI     BglI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsmBI
 Bsp1286I    BsrDI    BsrFI    BstXI   Bsu36I     BtgI     EaeI     EagI
     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII     KasI
   MspA1I     NaeI     NarI   NgoMIV     NotI    PpuMI   PspOMI     PstI
    PvuII    SacII     SfcI     SfoI    SgrAI     SmlI    TaqII     TstI
     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsaXI     BsgI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NcoI     NdeI
     NheI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com