Restriction map for pF1KB8959
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AfaI        
AflII       AflIII      AgeI        AlwNI       AocI        
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BamHI       
BbrPI       BbsI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaI        BsiEI       BsiWI       BsmFI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstXI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        EagI        EclXI       
Eco52I      Eco81I      EcoRI       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HpaI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
TspRI       Tth111I     XbaI        XcmI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII        402  TCCGGA               T^CCGGA
AcsI           58  AAATTC               [GA]^AATT[CT]
AhdI          535  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        331  GTGCAC               G^TGCAC
Aor51HI       575  AGCGCT               AGC^GCT
ApaLI         331  GTGCAC               G^TGCAC
ApoI           58  AAATTC               [GA]^AATT[CT]
AspEI         535  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          865  CTCGAG               C^[CT]CG[GA]G
AvaII         933  GGACC                G^G[AT]CC
BalI            5  TGGCCA               TGG^CCA
BbvCI         528  CCTCAGC              CCTCAGC(-5/-2)
BcgI          833  GCACGTCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          341  GCCTCGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          666  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      666  GCTGAGC              GC^T[ACGT]AGC
BsaWI         402  TCCGGA               [AT]^CCGG[AT]
BseAI         402  TCCGGA               T^CCGGA
BseRI         757  GAGGAG               GAGGAG(10/8)
BsmBI         195  CGTCTC               CGTCTC(1/5)
BsoBI         865  CTCGAG               C^[CT]CG[GA]G
BspEI         402  TCCGGA               T^CCGGA
BsrBI         387  GAGCGG               CCGCTC(-3/-3)
BsrFI         735  GCCGGT               [GA]^CCGG[CT]
BstEII        606  GGTGACC              G^GT[ACGT]ACC
BstPI         606  GGTGACC              G^GT[ACGT]ACC
CelII         666  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        735  GCCGGT               [GA]^CCGG[CT]
DraII         338  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      535  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          802  CTCTTC               CTCTTC(1/4)
Eco47III      575  AGCGCT               AGC^GCT
EcoNI         462  CCTGCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      338  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       606  GGTGACC              G^GT[ACGT]ACC
EcoRV         190  GATATC               GAT^ATC
HgaI          229  GACGC                GACGC(5/10)
HindIII       141  AAGCTT               A^AGCTT
Ksp632I       802  CTCTTC               CTCTTC(1/4)
MluNI           5  TGGCCA               TGG^CCA
MroI          402  TCCGGA               T^CCGGA
MscI            5  TGGCCA               TGG^CCA
MseI          938  TTAA                 T^TAA
MslI          474  CATGGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I        865  CTCGAG               C^TCGAG
PflMI         468  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          799  GAGCTC               GAGCT^C
SapI          801  GCTCTTC              GCTCTTC(1/4)
Tru9I         938  TTAA                 T^TAA
Tsp509I        59  AATT                 ^AATT
Van91I        468  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          865  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AlwI          429  GATCC                GGATC(4/5)
              648  GATCC               
BanI          448  GGCGCC               G^G[CT][GA]CC
              846  GGCGCC              
BanII         660  GGGCTC               G[GA]GC[CT]^C
              799  GAGCTC              
BbeI          448  GGCGCC               GGCGC^C
              846  GGCGCC              
BcnI           39  CCCGG                CC^[GC]GG
              652  CCCGG               
BsrI          930  ACTGG                ACTGG(1/-1)
               65  CCAGT               
BstAPI         36  GCACCCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              677  GCACCATCTGC         
BstF5I        214  CATCC                GGATG(2/0)
              433  CATCC               
BstYI         428  AGATCC               [GA]^GATC[CT]
              647  AGATCC              
EaeI            5  TGGCCA               [CT]^GGCC[GA]
              914  CGGCCA              
EcoT14I       452  CCATGG               C^C[AT][AT]GG
              473  CCATGG              
FokI          214  CATCC                GGATG(9/13)
              433  CATCC               
KasI          448  GGCGCC               G^GCGCC
              846  GGCGCC              
MaeIII        485  GTCAC                ^GT[ACGT]AC
              607  GTGAC               
MflI          428  AGATCC               [GA]^GATC[CT]
              647  AGATCC              
NarI          448  GGCGCC               GG^CGCC
              846  GGCGCC              
NciI           39  CCCGG                CC^[GC]GG
              652  CCCGG               
NcoI          452  CCATGG               C^CATGG
              473  CCATGG              
NspI           32  GCATGC               [GA]CATG^[CT]
              523  GCATGC              
PstI          121  CTGCAG               CTGCA^G
              684  CTGCAG              
PvuII         118  CAGCTG               CAG^CTG
              299  CAGCTG              
SfaNI         417  GATGC                GCATC(5/9)
              593  GCATC               
SfcI          121  CTGCAG               C^T[GA][CT]AG
              684  CTGCAG              
SfoI          448  GGCGCC               GGC^GCC
              846  GGCGCC              
SphI           32  GCATGC               GCATG^C
              523  GCATGC              
StuI          746  AGGCCT               AGG^CCT
              869  AGGCCT              
StyI          452  CCATGG               C^C[AT][AT]GG
              473  CCATGG              
Tsp45I        485  GTCAC                ^GT[GC]AC
              607  GTGAC               
XhoII         428  AGATCC               [GA]^GATC[CT]
              647  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AspHI       
BbvI        BmyI        BpmI        BsaHI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BspMI       BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraIII      DsaI        EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       SmlI        TaqI        TseI        TthHB8I     
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