Restriction map for pF1KB8958
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# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        AspEI       
AspI        AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
Hin1I       HindIII     HpaI        KasI        KspI        
MaeIII      MamI        MfeI        MluNI       MroI        
MscI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tsp45I      Tsp509I     Tth111I     TthHB8I     
XbaI        XhoI        


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# ONE CUT ENZYMES (   63 enzymes) #
###################################

Acc65I        365  GGTACC               G^GTACC
AflIII        332  ACGCGT               A^C[GA][CT]GT
AlwNI         505  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       365  GGTACC               G^GTACC
AspHI         499  GAGCAC               G[AT]GC[AT]^C
AviII         695  TGCGCA               TGC^GCA
BanI          365  GGTACC               G^G[CT][GA]CC
BanII         462  GGGCTC               G[GA]GC[CT]^C
BbsI          972  GTCTTC               GAAGAC(2/6)
BfaI          160  CTAG                 C^TAG
BglII         821  AGATCT               A^GATCT
BlpI           68  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       68  GCTCAGC              GC^T[ACGT]AGC
BpuAI         972  GTCTTC               GAAGAC(2/6)
BsaAI         522  TACGTA               [CT]AC^GT[GA]
BsgI          175  GTGCAG               GTGCAG(16/14)
BsiEI         150  CGACCG               CG[GA][CT]^CG
BsiHKAI       499  GAGCAC               G[AT]GC[AT]^C
BspMI          64  ACCTGC               ACCTGC(4/8)
BsrFI         228  GCCGGC               [GA]^CCGG[CT]
BssHII        765  GCGCGC               G^CGCGC
BstXI         483  CCACTCATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         821  AGATCT               [GA]^GATC[CT]
CelII          68  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        228  GCCGGC               [GA]^CCGG[CT]
DraII          61  GGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          413  CCATGG               C^C[GA][CT]GG
EaeI          890  CGGCCA               [CT]^GGCC[GA]
EarI          945  CTCTTC               CTCTTC(1/4)
EcoO109I       61  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       413  CCATGG               C^C[AT][AT]GG
FspI          695  TGCGCA               TGC^GCA
HaeII         127  AGCGCC               [GA]GCGC^[CT]
HgaI          762  GACGC                GACGC(5/10)
HincII        680  GTCAAC               GT[CT]^[GA]AC
HindII        680  GTCAAC               GT[CT]^[GA]AC
KpnI          365  GGTACC               GGTAC^C
Ksp632I       945  CTCTTC               CTCTTC(1/4)
MaeI          160  CTAG                 C^TAG
MflI          821  AGATCT               [GA]^GATC[CT]
MluI          332  ACGCGT               A^CGCGT
MseI          337  TTAA                 T^TAA
MslI          484  CACTCATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        383  CCGCTG               C[AC]G^C[GT]G
NaeI          228  GCCGGC               GCC^GGC
NcoI          413  CCATGG               C^CATGG
NdeI          488  CATATG               CA^TATG
NgoMIV        228  GCCGGC               G^CCGGC
NlaIII        414  CATG                 CATG^
PleI           55  GACTC                GAGTC(4/5)
PpuMI          61  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          796  CTGCAG               CTGCA^G
SapI          944  GCTCTTC              GCTCTTC(1/4)
SfcI          796  CTGCAG               C^T[GA][CT]AG
SmaI          966  CCCGGG               CCC^GGG
SmlI          276  CTCAAG               C^T[CT][GA]AG
SnaBI         522  TACGTA               TAC^GTA
StyI          413  CCATGG               C^C[AT][AT]GG
TfiI          925  GAATC                G^A[AT]TC
Tru9I         337  TTAA                 T^TAA
XhoII         821  AGATCT               [GA]^GATC[CT]
XmaCI         966  CCCGGG               C^CCGGG
XmaI          966  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

Asp700I        18  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              735  GAAGGAGTTC          
BmyI          462  GGGCTC               G[AGT]GC[ACT]^C
              499  GAGCAC              
BpmI          599  CTCCAG               CTGGAG(16/14)
              730  CTGGAG              
BsaI          808  GAGACC               GGTCTC(1/5)
              871  GGTCTC              
BsmFI          61  GGGAC                GGGAC(10/14)
              540  GTCCC               
Bsp1286I      462  GGGCTC               G[AGT]GC[ACT]^C
              499  GAGCAC              
BstF5I        791  CATCC                GGATG(2/0)
              620  GGATG               
FokI          791  CATCC                GGATG(9/13)
              620  GGATG               
HinfI          55  GACTC                G^A[ACGT]TC
              925  GAATC               
PflMI         289  CCAAGCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              504  CCAGAGCCTGG         
Van91I        289  CCAAGCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              504  CCAGAGCCTGG         
XcmI          273  CCACTCAAGTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              438  CCAGGGTTATGCTGG     
XmnI           18  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              735  GAAGGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaI        
AvaII       BbvI        BcnI        BsaJI       BsiYI       
BslI        BsmAI       BsoBI       BsrI        BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MaeII       MboI        MboII       
MnlI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TseI        TspRI       
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