Restriction map for pF1KB8958
 (Linear) (Six-base) MAPSORT of: KIBB8958.seq  Check: 9557  from: 1  to: 1005



KIBB8958.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

Acc65I G'GTAC_C

Cuts at:      0     365    1005 
   Size:        365     640
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294    1005 
   Size:        294     711
 
AflIII A'CryG_T

Cuts at:      0     332    1005 
   Size:        332     673
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     190    1005 
   Size:        190     815
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     724     756    1005 
   Size:        724      32     249

  Fragments arranged by size: 

                 724     249      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     510    1005 
   Size:        510     495
 
AvaI C'yCGr_G

Cuts at:      0      57      74     966    1005 
   Size:         57      17     892      39

  Fragments arranged by size: 

                 892      57      39      17
 
BanI G'GyrC_C

Cuts at:      0     365    1005 
   Size:        365     640
 
BanII G_rGCy'C

Cuts at:      0     466    1005 
   Size:        466     539
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     965    1005 
   Size:        965      40
 
BglII A'GATC_T

Cuts at:      0     821    1005 
   Size:        821     184
 
BlpI GC'TnA_GC

Cuts at:      0      69    1005 
   Size:         69     936
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     366    1005 
   Size:        366     639
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     945    1005 
   Size:        945      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     584     751    1005 
   Size:        584     167     254

  Fragments arranged by size: 

                 584     254     167
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     261    1005 
   Size:        261     744
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     802     877    1005 
   Size:        802      75     128

  Fragments arranged by size: 

                 802     128      75
 
BsaAI yAC'GTr

Cuts at:      0     524    1005 
   Size:        524     481
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     582     612     724     754    1005 
   Size:        582      30     112      30     251

  Fragments arranged by size: 

                 582     251     112      30      30
 
BseYI C'CCAG_C

Cuts at:      0     224     448    1005 
   Size:        224     224     557

  Fragments arranged by size: 

                 557     224     224
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196    1005 
   Size:        196     809
 
BsiEI CG_ry'CG

Cuts at:      0     153    1005 
   Size:        153     852
 
BsiHKAI G_wGCw'C

Cuts at:      0     503    1005 
   Size:        503     502
 
Bsp1286I G_dGCh'C

Cuts at:      0     466     503    1005 
   Size:        466      37     502

  Fragments arranged by size: 

                 502     466      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      73    1005 
   Size:         73     932
 
BsrFI r'CCGG_y

Cuts at:      0     228    1005 
   Size:        228     777
 
BssHII G'CGCG_C

Cuts at:      0     765    1005 
   Size:        765     240
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     490    1005 
   Size:        490     515
 
BstYI r'GATC_y

Cuts at:      0     821    1005 
   Size:        821     184
 
BtgI C'CryG_G

Cuts at:      0     413    1005 
   Size:        413     592
 
BtsI GCAGTG_nn'

Cuts at:      0      54    1005 
   Size:         54     951
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     262     297    1005 
   Size:        262      35     708

  Fragments arranged by size: 

                 708     262      35
 
EaeI y'GGCC_r

Cuts at:      0     890    1005 
   Size:        890     115
 
EarI CTCTTCn'nnn_

Cuts at:      0     951    1005 
   Size:        951      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     584     751    1005 
   Size:        294     290     167     254

  Fragments arranged by size: 

                 294     290     254     167
 
EcoO109I rG'GnC_Cy

Cuts at:      0      62    1005 
   Size:         62     943
 
FspI TGC'GCA

Cuts at:      0     697    1005 
   Size:        697     308
 
FspAI rTGC'GCAy

Cuts at:      0     697    1005 
   Size:        697     308
 
HaeII r_GCGC'y

Cuts at:      0     131    1005 
   Size:        131     874
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     582     614    1005 
   Size:        582      32     391

  Fragments arranged by size: 

                 582     391      32
 
HincII GTy'rAC

Cuts at:      0     682    1005 
   Size:        682     323
 
KpnI G_GTAC'C

Cuts at:      0     369    1005 
   Size:        369     636
 
MluI A'CGCG_T

Cuts at:      0     332    1005 
   Size:        332     673
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     639    1005 
   Size:        639     366
 
MslI CAynn'nnrTG

Cuts at:      0     488    1005 
   Size:        488     517
 
MspA1I CmG'CkG

Cuts at:      0     385    1005 
   Size:        385     620
 
NaeI GCC'GGC

Cuts at:      0     230    1005 
   Size:        230     775
 
NcoI C'CATG_G

Cuts at:      0     413    1005 
   Size:        413     592
 
NdeI CA'TA_TG

Cuts at:      0     489    1005 
   Size:        489     516
 
NgoMIV G'CCGG_C

Cuts at:      0     228    1005 
   Size:        228     777
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     295     510    1005 
   Size:        295     215     495

  Fragments arranged by size: 

                 495     295     215
 
PfoI T'CCnGG_A

Cuts at:      0      26     247    1005 
   Size:         26     221     758

  Fragments arranged by size: 

                 758     221      26
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     724     756    1005 
   Size:        724      32     249

  Fragments arranged by size: 

                 724     249      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      62    1005 
   Size:         62     943
 
PstI C_TGCA'G

Cuts at:      0     800    1005 
   Size:        800     205
 
SapI GCTCTTCn'nnn_

Cuts at:      0     951    1005 
   Size:        951      54
 
SfcI C'TryA_G

Cuts at:      0     796    1005 
   Size:        796     209
 
SmaI CCC'GGG

Cuts at:      0     968    1005 
   Size:        968      37
 
SmlI C'TyrA_G

Cuts at:      0     276    1005 
   Size:        276     729
 
SnaBI TAC'GTA

Cuts at:      0     524    1005 
   Size:        524     481
 
StyI C'CwwG_G

Cuts at:      0     413    1005 
   Size:        413     592
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     280     445    1005 
   Size:        280     165     560

  Fragments arranged by size: 

                 560     280     165
 
XmaI C'CCGG_G

Cuts at:      0     966    1005 
   Size:        966      39
 
XmnI GAAnn'nnTTC

Cuts at:      0      22     739    1005 
   Size:         22     717     266

  Fragments arranged by size: 

                 717     266      22
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AlfI     AloI    AlwNI     AvaI     BanI
    BanII     BbsI    BglII     BlpI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrFI   BssHII    BstXI    BstYI     BtgI     BtsI    CspCI
     EaeI     EarI  Eco57MI EcoO109I     FspI    FspAI    HaeII    Hin4I
   HincII     KpnI     MluI     MmeI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV    PflMI     PfoI     PpiI    PpuMI     PstI     SapI
     SfcI     SmaI     SmlI    SnaBI     StyI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI Bme1580I    BmgBI     BmtI   Bpu10I    BsaBI    BsaHI    BsaWI
    BseRI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI  HindIII     HpaI     KasI     MfeI
     MscI     NarI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     ZraI
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