Restriction map for pF1KB8919
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaI        BseRI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CpoI        DraI        DraIII      DrdI        Eam1105I    
EarI        Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FspI        HgaI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     KspI        
MaeIII      MfeI        MflI        MluI        MluNI       
MscI        MseI        MslI        MspA1I      MunI        
NdeI        NheI        NotI        NsiI        NspI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tru9I       Tsp45I      TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          403  GTCTAC               GT^[AC][GT]AC
AccIII         99  TCCGGA               T^CCGGA
AlwNI         148  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       470  AGCGCT               AGC^GCT
ApaI          220  GGGCCC               GGGCC^C
AspHI           9  GAGCTC               G[AT]GC[AT]^C
BbsI          730  GAAGAC               GAAGAC(2/6)
BclI            2  TGATCA               T^GATCA
BfaI          179  CTAG                 C^TAG
BpuAI         730  GAAGAC               GAAGAC(2/6)
BsaWI          99  TCCGGA               [AT]^CCGG[AT]
BseAI          99  TCCGGA               T^CCGGA
BsgI          204  CTGCAC               GTGCAG(16/14)
BsiHKAI         9  GAGCTC               G[AT]GC[AT]^C
BsmAI         212  GTCTC                GTCTC(1/5)
BsmFI         330  GTCCC                GGGAC(10/14)
BspDI          37  ATCGAT               AT^CGAT
BspEI          99  TCCGGA               T^CCGGA
BspHI           5  TCATGA               T^CATGA
ClaI           37  ATCGAT               AT^CGAT
DraII         219  GGGGCCC              [GA]G^G[ACGT]CC[CT]
Eco47III      470  AGCGCT               AGC^GCT
EcoNI         304  CCTCTCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      219  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       409  CCATGG               C^C[AT][AT]GG
FbaI            2  TGATCA               T^GATCA
FseI          245  GGCCGGCC             GGCCGG^CC
MaeI          179  CTAG                 C^TAG
MaeII         428  ACGT                 A^CGT
MroI           99  TCCGGA               T^CCGGA
NcoI          409  CCATGG               C^CATGG
NruI          232  TCGCGA               TCG^CGA
PaeR7I        474  CTCGAG               C^TCGAG
PstI          348  CTGCAG               CTGCA^G
RcaI            5  TCATGA               T^CATGA
SacI            9  GAGCTC               GAGCT^C
SexAI         784  ACCAGGT              A^CC[AT]GGT
SfaNI         138  GCATC                GCATC(5/9)
SfcI          348  CTGCAG               C^T[GA][CT]AG
SmaI          717  CCCGGG               CCC^GGG
SmlI          474  CTCGAG               C^T[CT][GA]AG
StyI          409  CCATGG               C^C[AT][AT]GG
XhoI          474  CTCGAG               C^TCGAG
XmaCI         717  CCCGGG               C^CCGGG
XmaI          717  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII         173  CGCG                 CG^CG
              233  CGCG                
AcsI           46  AAATTT               [GA]^AATT[CT]
              422  AAATTC              
AcyI          300  GGCGCC               G[GA]^CG[CT]C
              313  GGCGCC              
AfaI          156  GTAC                 GT^AC
              722  GTAC                
AlwI           40  GATCC                GGATC(4/5)
              557  GGATC               
ApoI           46  AAATTT               [GA]^AATT[CT]
              422  AAATTC              
AvaII         116  GGACC                G^G[AT]CC
              639  GGACC               
BanI          300  GGCGCC               G^G[CT][GA]CC
              313  GGCGCC              
BanII           9  GAGCTC               G[GA]GC[CT]^C
              220  GGGCCC              
BbeI          300  GGCGCC               GGCGC^C
              313  GGCGCC              
BmyI            9  GAGCTC               G[AGT]GC[ACT]^C
              220  GGGCCC              
BpmI          214  CTCCAG               CTGGAG(16/14)
              375  CTCCAG              
BsaBI         558  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              597  GATCAAAATC          
BsaHI         300  GGCGCC               G[GA]^CG[CT]C
              313  GGCGCC              
BsiEI         111  CGGCCG               CG[GA][CT]^CG
              680  CGGCCG              
Bsp1286I        9  GAGCTC               G[AGT]GC[ACT]^C
              220  GGGCCC              
BsrI          195  CCAGT                ACTGG(1/-1)
              209  CCAGT               
BstUI         173  CGCG                 CG^CG
              233  CGCG                
DsaI          240  CCACGG               C^C[GA][CT]GG
              409  CCATGG              
EaeI          111  CGGCCG               [CT]^GGCC[GA]
              680  CGGCCG              
EagI          111  CGGCCG               C^GGCCG
              680  CGGCCG              
EclXI         111  CGGCCG               C^GGCCG
              680  CGGCCG              
Eco52I        111  CGGCCG               C^GGCCG
              680  CGGCCG              
FauI          339  CCCGC                CCCGC(4/6)
              256  GCGGG               
Hin1I         300  GGCGCC               G[GA]^CG[CT]C
              313  GGCGCC              
HphI          438  GGTGA                GGTGA(8/7)
              141  TCACC               
KasI          300  GGCGCC               G^GCGCC
              313  GGCGCC              
MamI          558  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              597  GATCAAAATC          
MvnI          173  CGCG                 CG^CG
              233  CGCG                
NarI          300  GGCGCC               GG^CGCC
              313  GGCGCC              
PleI           25  GACTC                GAGTC(4/5)
              647  GACTC               
RsaI          156  GTAC                 GT^AC
              722  GTAC                
SfoI          300  GGCGCC               GGC^GCC
              313  GGCGCC              
SspI           65  AATATT               AAT^ATT
              104  AATATT              
TfiI          124  GAATC                G^A[AT]TC
              131  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AluI        AvaI        BbvI        BcnI        
BsaJI       BsiYI       BslI        BsoBI       BsrFI       
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
TaqI        TseI        Tsp509I     TthHB8I     
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