Restriction map for pF1KB8911
 (Linear) (Six-base) MAPSORT of: KIBB8911.seq  Check: 5381  from: 1  to: 750



KIBB8911.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0      35     230     750 
   Size:         35     195     520

  Fragments arranged by size: 

                 520     195      35
 
Acc65I G'GTAC_C

Cuts at:      0     278     750 
   Size:        278     472
 
AfeI AGC'GCT

Cuts at:      0     565     750 
   Size:        565     185
 
AflIII A'CryG_T

Cuts at:      0     206     750 
   Size:        206     544
 
AleI CACnn'nnGTG

Cuts at:      0      56     750 
   Size:         56     694
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     598     630     750 
   Size:        598      32     120

  Fragments arranged by size: 

                 598     120      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     591     750 
   Size:        591     159
 
ApoI r'AATT_y

Cuts at:      0     476     750 
   Size:        476     274
 
AscI GG'CGCG_CC

Cuts at:      0     368     750 
   Size:        368     382
 
AvaI C'yCGr_G

Cuts at:      0     324     400     750 
   Size:        324      76     350

  Fragments arranged by size: 

                 350     324      76
 
BanI G'GyrC_C

Cuts at:      0     178     189     278     303     750 
   Size:        178      11      89      25     447

  Fragments arranged by size: 

                 447     178      89      25      11
 
BanII G_rGCy'C

Cuts at:      0     106     202     750 
   Size:        106      96     548

  Fragments arranged by size: 

                 548     106      96
 
BbeI G_GCGC'C

Cuts at:      0     182     193     307     750 
   Size:        182      11     114     443

  Fragments arranged by size: 

                 443     182     114      11
 
BglI GCCn_nnn'nGGC

Cuts at:      0     187     750 
   Size:        187     563
 
BlpI GC'TnA_GC

Cuts at:      0      19     196     750 
   Size:         19     177     554

  Fragments arranged by size: 

                 554     177      19
 
Bme1580I G_kGCm'C

Cuts at:      0     397     750 
   Size:        397     353
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     159     750 
   Size:        159     591
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     154     316     613     750 
   Size:         56      98     162     297     137

  Fragments arranged by size: 

                 297     162     137      98      56
 
BsaHI Gr'CG_yC

Cuts at:      0     179     190     257     304     750 
   Size:        179      11      67      47     446

  Fragments arranged by size: 

                 446     179      67      47      11
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     598     628     750 
   Size:        598      30     122

  Fragments arranged by size: 

                 598     122      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     652     750 
   Size:        652      98
 
BseYI C'CCAG_C

Cuts at:      0     363     750 
   Size:        363     387
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     523     750 
   Size:        168     355     227

  Fragments arranged by size: 

                 355     227     168
 
BsiEI CG_ry'CG

Cuts at:      0     269     750 
   Size:        269     481
 
BsiHKAI G_wGCw'C

Cuts at:      0     106     131     750 
   Size:        106      25     619

  Fragments arranged by size: 

                 619     106      25
 
Bsp1286I G_dGCh'C

Cuts at:      0     106     131     202     397     750 
   Size:        106      25      71     195     353

  Fragments arranged by size: 

                 353     195     106      71      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     266     750 
   Size:        266     484
 
BsrFI r'CCGG_y

Cuts at:      0     168     264     300     704     750 
   Size:        168      96      36     404      46

  Fragments arranged by size: 

                 404     168      96      46      36
 
BssHII G'CGCG_C

Cuts at:      0     368     750 
   Size:        368     382
 
BssSI C'ACGA_G

Cuts at:      0      52     144     750 
   Size:         52      92     606

  Fragments arranged by size: 

                 606      92      52
 
DraIII CAC_nnn'GTG

Cuts at:      0     651     750 
   Size:        651      99
 
EaeI y'GGCC_r

Cuts at:      0      90     266     653     750 
   Size:         90     176     387      97

  Fragments arranged by size: 

                 387     176      97      90
 
EagI C'GGCC_G

Cuts at:      0     266     750 
   Size:        266     484
 
EarI CTCTTCn'nnn_

Cuts at:      0     555     750 
   Size:        555     195
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     367     516     723     750 
   Size:        367     149     207      27

  Fragments arranged by size: 

                 367     207     149      27
 
EcoICRI GAG'CTC

Cuts at:      0     104     750 
   Size:        104     646
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     154     316     613     750 
   Size:         56      98     162     297     137

  Fragments arranged by size: 

                 297     162     137      98      56
 
HaeII r_GCGC'y

Cuts at:      0     169     182     193     307     567     750 
   Size:        169      13      11     114     260     183

  Fragments arranged by size: 

                 260     183     169     114      13      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      33      65     495     527     750 
   Size:         33      32     430      32     223

  Fragments arranged by size: 

                 430     223      33      32      32
 
HincII GTy'rAC

Cuts at:      0      36     750 
   Size:         36     714
 
KasI G'GCGC_C

Cuts at:      0     178     189     303     750 
   Size:        178      11     114     447

  Fragments arranged by size: 

                 447     178     114      11
 
KpnI G_GTAC'C

Cuts at:      0     282     750 
   Size:        282     468
 
MluI A'CGCG_T

Cuts at:      0     206     750 
   Size:        206     544
 
MscI TGG'CCA

Cuts at:      0      92     655     750 
   Size:         92     563      95

  Fragments arranged by size: 

                 563      95      92
 
MslI CAynn'nnrTG

Cuts at:      0      56     750 
   Size:         56     694
 
MspA1I CmG'CkG

Cuts at:      0     195     360     750 
   Size:        195     165     390

  Fragments arranged by size: 

                 390     195     165
 
NaeI GCC'GGC

Cuts at:      0     266     302     750 
   Size:        266      36     448

  Fragments arranged by size: 

                 448     266      36
 
NarI GG'CG_CC

Cuts at:      0     179     190     304     750 
   Size:        179      11     114     446

  Fragments arranged by size: 

                 446     179     114      11
 
NgoMIV G'CCGG_C

Cuts at:      0     264     300     750 
   Size:        264      36     450

  Fragments arranged by size: 

                 450     264      36
 
PasI CC'CwG_GG

Cuts at:      0     252     750 
   Size:        252     498
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     591     750 
   Size:        591     159
 
PfoI T'CCnGG_A

Cuts at:      0     211     553     750 
   Size:        211     342     197

  Fragments arranged by size: 

                 342     211     197
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     598     630     750 
   Size:        598      32     120

  Fragments arranged by size: 

                 598     120      32
 
SacI G_AGCT'C

Cuts at:      0     106     750 
   Size:        106     644
 
SalI G'TCGA_C

Cuts at:      0      34     750 
   Size:         34     716
 
SapI GCTCTTCn'nnn_

Cuts at:      0     555     750 
   Size:        555     195
 
SfoI GGC'GCC

Cuts at:      0     180     191     305     750 
   Size:        180      11     114     445

  Fragments arranged by size: 

                 445     180     114      11
 
SgrAI Cr'CCGG_yG

Cuts at:      0     168     750 
   Size:        168     582
 
SmaI CCC'GGG

Cuts at:      0     326     750 
   Size:        326     424
 
TatI w'GTAC_w

Cuts at:      0     404     750 
   Size:        404     346
 
XmaI C'CCGG_G

Cuts at:      0     324     750 
   Size:        324     426
 

 Enzymes that do cut:

     AccI   Acc65I     AfeI   AflIII     AleI     AloI    AlwNI     ApoI
     AscI     AvaI     BanI    BanII     BbeI     BglI     BlpI Bme1580I
     BplI     BpmI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrFI   BssHII    BssSI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     PasI    PflMI     PfoI     PpiI     SacI     SalI     SapI
     SfoI    SgrAI     SmaI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI    AflII     AgeI     AhdI     AlfI
     ApaI    ApaLI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     MfeI
     MmeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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