Restriction map for pF1KB8901
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BbeI        BbrPI       BbsI        BcgI        BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HpaI        KasI        MaeII       MamI        
MfeI        MluI        MroI        MseI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        299  GGTACC               G^GTACC
AccI          463  GTAGAC               GT^[AC][GT]AC
AccII         189  CGCG                 CG^CG
AfaI          300  GTAC                 GT^AC
Aor51HI       264  AGCGCT               AGC^GCT
Asp718I       299  GGTACC               G^GTACC
AvaI          230  CCCGGG               C^[CT]CG[GA]G
BamHI         542  GGATCC               G^GATCC
BanII         678  GGGCTC               G[GA]GC[CT]^C
BbvCI         267  GCTGAGG              CCTCAGC(-5/-2)
BfaI          532  CTAG                 C^TAG
BglI          608  GCCGAAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI           69  CTGGAG               CTGGAG(16/14)
BsaI          136  GAGACC               GGTCTC(1/5)
BsmI          310  GCATTC               GAATGC(1/-1)
BsoBI         230  CCCGGG               C^[CT]CG[GA]G
BspMI         477  GCAGGT               ACCTGC(4/8)
BstUI         189  CGCG                 CG^CG
BstYI         542  GGATCC               [GA]^GATC[CT]
CfoI          265  GCGC                 GCG^C
DraII         107  AGGCCCC              [GA]G^G[ACGT]CC[CT]
Eco47III      264  AGCGCT               AGC^GCT
EcoO109I      107  AGGCCCC              [GA]G^G[ACGT]CC[CT]
HaeII         264  AGCGCT               [GA]GCGC^[CT]
HhaI          265  GCGC                 GCG^C
HinP1I        265  GCGC                 G^CGC
HincII         28  GTTGAC               GT[CT]^[GA]AC
HindII         28  GTTGAC               GT[CT]^[GA]AC
HindIII        46  AAGCTT               A^AGCTT
KpnI          299  GGTACC               GGTAC^C
KspI          188  CCGCGG               CCGC^GG
MaeI          532  CTAG                 C^TAG
MaeIII        317  GTGAC                ^GT[ACGT]AC
MflI          542  GGATCC               [GA]^GATC[CT]
MslI          651  CATGACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          189  CGCG                 CG^CG
NaeI          661  GCCGGC               GCC^GGC
NcoI          245  CCATGG               C^CATGG
NgoMIV        661  GCCGGC               G^CCGGC
PvuII          66  CAGCTG               CAG^CTG
RsaI          300  GTAC                 GT^AC
SacII         188  CCGCGG               CCGC^GG
SmaI          230  CCCGGG               CCC^GGG
TfiI          124  GAATC                G^A[AT]TC
Tsp45I        317  GTGAC                ^GT[GC]AC
XhoII         542  GGATCC               [GA]^GATC[CT]
XmaCI         230  CCCGGG               C^CCGGG
XmaI          230  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BalI          207  TGGCCA               TGG^CCA
              509  TGGCCA              
BanI          170  GGCACC               G^G[CT][GA]CC
              299  GGTACC              
BciVI          61  GGATAC               GTATCC(6/5)
              564  GGATAC              
BmyI          443  GGGCAC               G[AGT]GC[ACT]^C
              678  GGGCTC              
BsmAI         136  GAGAC                GTCTC(1/5)
                3  GTCTC               
BsmFI         118  GGGAC                GGGAC(10/14)
              349  GGGAC               
Bsp1286I      443  GGGCAC               G[AGT]GC[ACT]^C
              678  GGGCTC              
BsrFI         302  ACCGGC               [GA]^CCGG[CT]
              661  GCCGGC              
Cfr10I        302  ACCGGC               [GA]^CCGG[CT]
              661  GCCGGC              
Cfr13I        108  GGCCC                G^G[ACGT]CC
              615  GGGCC               
DsaI          188  CCGCGG               C^C[GA][CT]GG
              245  CCATGG              
EcoT14I       245  CCATGG               C^C[AT][AT]GG
              538  CCTTGG              
MluNI         207  TGGCCA               TGG^CCA
              509  TGGCCA              
MscI          207  TGGCCA               TGG^CCA
              509  TGGCCA              
NlaIII        246  CATG                 CATG^
              651  CATG                
PleI           72  GAGTC                GAGTC(4/5)
              489  GAGTC               
Sau96I        108  GGCCC                G^G[ACGT]CC
              615  GGGCC               
SfaNI         226  GATGC                GCATC(5/9)
              474  GATGC               
StyI          245  CCATGG               C^C[AT][AT]GG
              538  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BcnI        
BsaJI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       Cac8I       DdeI        DpnII       
EaeI        EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIV       Sau3AI      ScrFI       
TseI        TspRI       
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