Restriction map for pF1KB8890
##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        Fnu4HI      FseI        FspI        HaeII       
HapII       Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        HpaII       ItaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StyI        SwaI        TaqI        TfiI        TseI        
TthHB8I     XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AflII         178  CTTAAG               C^TTAAG
AluI          575  AGCT                 AG^CT
ApaI          212  GGGCCC               GGGCC^C
Asp700I       558  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          538  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         375  GGACC                G^G[AT]CC
BanII         212  GGGCCC               G[GA]GC[CT]^C
BfaI          595  CTAG                 C^TAG
BfrI          178  CTTAAG               C^TTAAG
BpmI           11  CTCCAG               CTGGAG(16/14)
BsaI          543  GGTCTC               GGTCTC(1/5)
Bsp1407I      434  TGTACA               T^GTACA
BspHI         419  TCATGA               T^CATGA
BsrDI         321  CATTGC               GCAATG(2/0)
BsrGI         434  TGTACA               T^GTACA
BssSI         502  CTCGTG               CACGAG(-5/-1)
CfoI          255  GCGC                 GCG^C
DpnII          85  GATC                 ^GATC
DraII         211  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      211  AGGGCCC              [GA]G^G[ACGT]CC[CT]
FauI          526  CCCGC                CCCGC(4/6)
HgaI          218  GACGC                GACGC(5/10)
HhaI          255  GCGC                 GCG^C
HinP1I        255  GCGC                 G^CGC
MaeI          595  CTAG                 C^TAG
MboI           85  GATC                 ^GATC
MspA1I        527  CCGCTG               C[AC]G^C[GT]G
NdeII          85  GATC                 ^GATC
PflFI         538  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI          419  TCATGA               T^CATGA
Sau3AI         85  GATC                 ^GATC
SmlI          178  CTTAAG               C^T[CT][GA]AG
SspBI         434  TGTACA               T^GTACA
StuI          482  AGGCCT               AGG^CCT
Tsp509I       509  AATT                 ^AATT
Tth111I       538  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          558  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI           60  GTAC                 GT^AC
              435  GTAC                
BsmFI         264  GGGAC                GGGAC(10/14)
              396  GGGAC               
BsrI           34  ACTGG                ACTGG(1/-1)
               13  CCAGT               
BstF5I        105  CATCC                GGATG(2/0)
              450  CATCC               
EcoNI          63  CCTGTTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              174  CCTCCTTAAGG         
FokI          105  CATCC                GGATG(9/13)
              450  CATCC               
MboII         423  GAAGA                GAAGA(8/7)
              558  GAAGA               
PflMI         159  CCATGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              294  CCATGCCCTGG         
RsaI           60  GTAC                 GT^AC
              435  GTAC                
TspRI          33  CACTG                CA[GC]TG[ACGT][ACGT]^
              477  CACTG               
Van91I        159  CCATGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              294  CCATGCCCTGG         
XcmI           44  CCATTAACATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              108  CCACCGCCTTCGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        BanI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       BstNI       
Cac8I       Cfr13I      DdeI        EcoRII      HaeIII      
HphI        MaeIII      MnlI        MseI        MslI        
MvaI        NlaIII      NlaIV       Sau96I      ScrFI       
SfaNI       Tru9I       Tsp45I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com